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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2668 [new locus tag: SACOL_RS13975 ]
  • pan locus tag?: SAUPAN006379000
  • symbol: SACOL2668
  • pan gene symbol?: sasF
  • synonym:
  • product: LPXTG cell wall surface anchor family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2668 [new locus tag: SACOL_RS13975 ]
  • symbol: SACOL2668
  • product: LPXTG cell wall surface anchor family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2735299..2737206
  • length: 1908
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTAAATATCGAGGGAAACCGTTTCAATTATATGTAAAGTTATCGTGTTCGACAATG
    ATGGCGACAAGTATCATTTTAACGAATATCTTGCCGTACGATGCCCAAGCTGCATCTGAA
    AAGGATACTGAAATTACAAAAGAGATATTATCTAAGCAAGATTTATTAGACAAAGTTGAC
    AAGGCAATTCGTCAAATTGAGCAATTAAAACAGTTATCGGCTTCATCTAAAGAACATTAT
    AAAGCACAACTAAATGAAGCGAAAACAGCATCGCAAATAGATGAAATCATAAAACGAGCT
    AATGAGTTGGATAGCAAAGACAATAAAAGTTCTCACACTGAAATGAACGGTCAAAGTGAT
    ATAGACAGTAAATTAGATCAATTGCTTAAAGATTTAAATGAGGTTTCTTCAAATGTTGAT
    AGGGGTCAACAAAGTGGCGAGGACGATCTTAATGCAATGAAAAATGATATGTCACAAACG
    GCTACAACAAAACATGGAGAAAAAGATGATAAAAATGATGAAGCAATGGTAAATAAGGCG
    TTAGAAGACCTAGACCATTTGAATCAGCAAATACACAAATCGAAAGATGCATCGAAAGAT
    ACATCGGAAGATCCAGCAGTGTCTACAACAGATAATAATCATGAAGTAGCTAAAACGCCA
    AATAATGATGGTTCTGGACATGTTGTGTTAAATAAATTCCTTTCAAATGAAGAGAATCAA
    AGCCATAGTAATCGACTCACTGATAAATTACAAGGAAGCGATAAAATTAATCATGCTATG
    ATTGAAAAATTAGCTAAAAGTAATGCCTCAACGCAACATTACACATATCATAAACTGAAT
    ACGTTACAATCTTTAGATCAACGTATTGCAAATACGCAACTTCCTAAAAATCAAAAATCA
    GACTTAATGAGCGAAGTAAATAAGACGAAAGAGCGTATAAAAAGTCAACGAAATATTATT
    TTGGAAGAACTTGCACGTACTGATGATAAAAAGTATGCTACACAAAGCATTTTAGAAAGT
    ATATTTAATAAAGACGAGGCAGTTAAAATTCTAAAAGATATACGTGTTGATGGTAAAACA
    GATCAACAAATTGCAGATCAAATTACTCGTCATATTGATCAATTATCTCTGACAACGAGT
    GATGATTTATTAACGTCATTGATTGATCAATCACAAGATAAGTCGCTATTGATTTCTCAA
    ATTTTACAAACGAAATTAGGAAAAGCTGAAGCAGATAAATTGGCTAAAGATTGGACGAAT
    AAAGGATTATCAAATCGCCAAATCGTTGACCAATTGAAGAAACATTTTGCATCAACTGGC
    GACACGTCTTCAGATGATATATTAAAAGCAATTTTGAATAATGCCAAAGATAAAAAACAA
    GCAATTGAAACGATTTTAGCAACACGTATAGAAAGACAAAAGGCAAAATTACTGGCAGAT
    TTAATTACTAAAATAGAAACAGATCAAAATAAAATTTTTAATTTAGTTAAATCGGCATTG
    AATGGTAAAGCGGATGATTTATTGAATTTACAAAAGAGACTCAATCAAACGAAAAAAGAT
    ATAGATTATATTTTATCACCAATAGTAAATCGTCCAAGTTTACTAGATCGATTGAATAAA
    AATGGGAAAACGACAGATTTAAATAAGTTAGCAAATTTAATGAATCAAGGATCAGATTTA
    TTAGACAGTATTCCAGATATACCCACACCAAAGCCAGAAAAGACGTTAACACTTGGTAAA
    GGTAATGGATTGTTAAGTGGATTATTAAATGCTGATGGTAATGTATCTTTGCCTAAAGCG
    GGGGAAACGATAAAAGAACATTGGTTGCCGATATCTGTAATTGTTGGTGCAATGGGTGTA
    CTAATGATTTGGTTATCACGACGCAATAAGTTGAAAAATAAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1908

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2668 [new locus tag: SACOL_RS13975 ]
  • symbol: SACOL2668
  • description: LPXTG cell wall surface anchor family protein
  • length: 635
  • theoretical pI: 8.35733
  • theoretical MW: 70931.7
  • GRAVY: -0.749449

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Predicted cell-wall-anchored protein SasF (LPXAG motif) 
  • PFAM:
    B_GA (CL0598) GA; GA module (PF01468; HMM-score: 16.2)
    HHH (CL0198) HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 14.6)
    no clan definedPoly_export; Polysaccharide biosynthesis/export protein (PF02563; HMM-score: 13.5)
    COX5A; Cytochrome c oxidase subunit Va (PF02284; HMM-score: 10.1)
    SAP30_Sin3_bdg; Sin3 binding region of histone deacetylase complex subunit SAP30 (PF13867; HMM-score: 8.8)
    B2; RNA binding protein B2 (PF11473; HMM-score: 8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: found LPxTG motif :LPKAG
  • SignalP: Signal peptide length 38 aa
    • Ymax: 0.635
    • Ymax_pos: 38
    • Cmax: 0.652
    • Cmax_pos: 38
    • Smax: 0.763
    • Smax_pos: 34
    • Smean: 0.534
    • D: 0.595
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTEITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANELDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQTATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAKTPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTYHKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYATQSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQDKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKAILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLLNLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPDIPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIWLSRRNKLKNKA

Experimental data[edit source | edit]

  • experimental localization: Sortase substrate [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]