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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS01240 [old locus tag: SACOL0245 ]
  • pan locus tag?: SAUPAN001139000
  • symbol: SACOL_RS01240
  • pan gene symbol?: lytS
  • synonym:
  • product: sensor histidine kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS01240 [old locus tag: SACOL0245 ]
  • symbol: SACOL_RS01240
  • product: sensor histidine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 285439..287178
  • length: 1740
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (285439..287178) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTTATTACTTGAGCGTGTAGGTTTAATTATTATTTTGGCCTATGTGTTGATGAATATT
    CCATATTTTAAAAACTTAATGAATCGTCGACGTACATGGAAAGCACGTTGGCAATTATGT
    ATTATTTTCAGTTTGTTTGCCTTAATGTCTAATTTAACTGGTATCGTCATCGATCATCAA
    CATAGTTTGTCAGGAAGTGTGTACTTCCGTTTAGATGATGATGTATCTTTAGCTAACACA
    CGTGTATTAACGATAGGTGTCGCAGGATTAGTTGGTGGCCCTTTTGTAGGTCTATTTGTT
    GGCGTTATTTCAGGTATTTTCAGAGTGTATATGGGTGGGGCGGATGCACAAGTTTATCTT
    ATCTCATCTATATTTATTGGTATAATTGCTGGTTATTTTGGCTTACAAGCTCAAAGACGC
    AAGCGTTACCCGAGTATTGCGAAAAGTGCCATGATTGGAATTGTTATGGAAATGATTCAA
    ATGTTGAGCATTTTAACATTTTCCCACGACAAAGCATATGCGGTTGACCTCATATCATTA
    ATTGCACTACCAATGATTATTGTTAATAGCGTTGGTACGGCGATTTTTATGTCTATTATC
    ATTTCAACATTAAAGCAAGAGGAGCAAATGAAGGCTGTTCAAACACATGATGTACTGCAA
    TTGATGAACCAGACATTGCCGTATTTTAAAGAAGGATTGAATAGAGAATCGGCACAGCAA
    ATTGCGATGATTATTAAAAATTTAATGAAAGTATCTGCCGTAGCAATTACAAGCAAAAAT
    GAAATCTTATCGCATGTAGGTGCAGGTAGTGATCATCACATACCAACAAATGAAATATTA
    ACAAGTCTGTCTAAAGATGTATTGAAATCAGGAAAGTTGAAAGAAGTTCATACTAAAGAA
    GAGATTGGTTGTAGTCATCCGAATTGCCCGCTTAGAGCAGCTATCGTGATACCACTTGAG
    ATGCATGGTTCTATCGTCGGTACATTGAAGATGTATTTTACAAACCCTAATGATTTAACT
    TTTGTGGAACGTCAACTTGCAGAAGGATTGGCAAATATTTTTAGTAGCCAAATTGAACTT
    GGTGAAGCCGAAACGCAAAGTAAGTTATTGAAAGATGCTGAGATTAAGTCATTACAGGCA
    CAAGTGAGTCCACATTTTTTCTTCAATTCAATTAACACGATCTCAGCTTTAGTTAGAATA
    AATAGCGAAAAGGCACGAGAGTTACTATTAGAATTGAGTTATTTTTTCAGAGCGAATTTA
    CAAGGCTCTAAGCAACATACGATTACTTTAGATAAAGAGTTAAGTCAAGTGCGTGCATAC
    TTATCACTCGAACAAGCACGTTATCCAGGAAGATTTAATATCAATATTAATGTTGAAGAC
    AAATATCGCGATGTGCTTGTACCACCATTTTTAATTCAAATTTTAGTTGAAAATGCCATC
    AAACATGCGTTTACGAATCGAAAGCAAGGTAACGATATTGACGTGTCAGTGATTAAAGAA
    ACTGCAACACATGTACGTATTATTGTACAAGATAATGGTCAGGGTATTTCTAAAGATAAA
    ATGCATTTGTTGGGAGAAACATCTGTAGAATCAGAGTCTGGAACTGGTAGTGCTTTAGAA
    AATTTAAACTTACGCCTAAAAGGATTATTTGGAAAATCCGCAGCATTACAATTTGAATCG
    ACATCGAGCGGTACCACTTTTTGGTGTGTACTTCCTTATGAAAGACAAGAGGAGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS01240 [old locus tag: SACOL0245 ]
  • symbol: SACOL_RS01240
  • description: sensor histidine kinase
  • length: 579
  • theoretical pI: 8.30556
  • theoretical MW: 64482.6
  • GRAVY: 0.138342

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 21.7)
    putative exosortase-associated protein, TIGR04073 family (TIGR04073; HMM-score: 10.9)
  • TheSEED:
  • PFAM:
    Gx_transp (CL0315) 5TM-5TMR_LYT; 5TMR of 5TMR-LYT (PF07694; HMM-score: 176.1)
    no clan definedHis_kinase; Histidine kinase (PF06580; HMM-score: 79.1)
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 42.3)
    Gx_transp (CL0315) ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 27)
    GAF (CL0161) GAF_3; GAF domain (PF13492; HMM-score: 23.8)
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 18.4)
    GAF (CL0161) GAF_2; GAF domain (PF13185; HMM-score: 14.2)
    no clan definedEMP70; Endomembrane protein 70 (PF02990; HMM-score: 8.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.137
    • Ymax_pos: 46
    • Cmax: 0.124
    • Cmax_pos: 24
    • Smax: 0.234
    • Smax_pos: 38
    • Smean: 0.114
    • D: 0.128
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_000925394 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQLCIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVGLFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIVMEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAVQTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSDHHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTLKMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFFFNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETATHVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFESTSSGTTFWCVLPYERQEEE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS01240 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]