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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS01325 [old locus tag: SACOL0261 ]
  • pan locus tag?: SAUPAN001161000
  • symbol: SACOL_RS01325
  • pan gene symbol?:
  • synonym:
  • product: MFS transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS01325 [old locus tag: SACOL0261 ]
  • symbol: SACOL_RS01325
  • product: MFS transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 298738..300114
  • length: 1377
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (298738..300114) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    GTGAATAACACACAATCTTCACCACGCAGTAATATTATTATTGCGATTATGTTGTCTGCA
    TTAACATATTGGTTGTTTGCACAATCATTTATTAATATAGGACCTCTCGTTGGTCAAACG
    TATCAAACCTCTCCTGCCGTGTTAAATTTATCTATTAGTTTAACTTCCTTCGCCACAGGT
    ATCTTCATGGTGGCTGCAGGTGATATTGCTGATAAAATAGGACAACTGAGAATGACATAC
    ATGGGTCTCATAATCAGTATGTTTGCATCTCTTCTATTAATTATATCGGACATCACTGCA
    CTGCTCATCATCGGTAGAATTTTACAAGGTCTATCAGCAGCTATCTTGTTACCTTCAACA
    GTTGGCGTGTTAAATAATCAATTTAAAGGAGAACATTTAAGACGAGCGATTAGTTATCTA
    ATGATTAGTACTGTTGGTGGCATCGGCCTAGCTGGTGTTATCGGCGGTTTAATTGCCTCA
    AATTTCGGATGGCAAACGAATTTCATCATTAGTATAGTCATTGCTTTCATTGCCATATTG
    CTTCTAAAAGGCACACCTGAAAAAGTAAGTCAACATAGCCACCGTCATCCATTCGATTAC
    AAAGGTATGTCGATTTTCGCTGTTATGATTGGTAGCTTTACATTATTGTTAACACAAGGA
    TTCGAACAAGGTTGGTTTAGTACATTTTCAATCATTTGTCTGAGCATTTTTATCATCACT
    ACGTTGATATTCATCATCATCGAACGTCGACATGAAGTACCTTTTATTGATTTCTCAGTA
    TTACGCAACCGTCCGTTCATTGGTGCATTTTTAAATAACTTTGTTTTAAATAGCGGTCTA
    GGCGTAACAGTGGTCTTTTTCATATATGCTCAAACACACCTTGGTTTATCAGCTGCGCAA
    TCTGGACTTGTTACATTGCCATATGCCATTGTGGCAGTTGCGATGATTCGTTTAGGTGAA
    AAAGCAACATTACGTTTCGGTGGCAAATTGATGCTCATCATTGGTCCGTTGTTCCCTGTC
    ATCGGCATCACTATTATTAGCATGACTCAATTATTGGCATCACAATATGTCATTGCAGTT
    ATCATTGGTTTCGTCATATGTGCGATAGGTAATGGTTTAGTCGCAACACCTGGACTTACG
    ATTGCAATTTTCAGTATGCCTAATGAAAAAGTTGGTTTAGCTACAGGATTATATAAAATG
    AGTGGTACATTAGGTGGCGCCTTTGGTATAGCACTAAGTACTACAGTTTTCAGTATGTTA
    CAACTAAACTATGCACCAAGTGTAGCTGCAACCGTAACATTTATAGTCAGCATTGTATTG
    ATGATCCTTGGCTCATTGTCTGCATACATGATCATTCCAAAAACAGTTAAATCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1377

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS01325 [old locus tag: SACOL0261 ]
  • symbol: SACOL_RS01325
  • description: MFS transporter
  • length: 458
  • theoretical pI: 10.1683
  • theoretical MW: 49040.3
  • GRAVY: 0.980568

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 131.7)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 131.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 56.2)
    multidrug resistance protein (TIGR00880; HMM-score: 45.4)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 40.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 31.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 28.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsoxalate/formate antiporter family transporter (TIGR00890; HMM-score: 21.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidssugar efflux transporter (TIGR00899; HMM-score: 18)
  • TheSEED:
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 138.1)
    no clan definedUL42; HCMV UL42 (PF17638; HMM-score: 13.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.191
    • Ymax_pos: 12
    • Cmax: 0.18
    • Cmax_pos: 30
    • Smax: 0.453
    • Smax_pos: 3
    • Smean: 0.369
    • D: 0.261
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit source | edit]

  • GI: 446989289 NCBI
  • RefSeq: WP_001066545 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIASNFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS01325 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]