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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS01975 [old locus tag: SACOL0392 ]
  • pan locus tag?: SAUPAN001852000
  • symbol: SACOL_RS01975
  • pan gene symbol?:
  • synonym:
  • product: NADH-dependent flavin oxidoreductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS01975 [old locus tag: SACOL0392 ]
  • symbol: SACOL_RS01975
  • product: NADH-dependent flavin oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 399550..400749
  • length: 1200
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (399550..400749) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    ATGTACCGATACAAACCACTTTTACAATCAATCCACTTACCTAATGGCATCAAAATTTCG
    AATCGCTTTGTATTATCACCAATGACCGTAAACACATCAACAAAAGAAGGCTATATTACA
    AAAGAAGACTTGGCTTATGCTGCACGTCGCTCTAATTCAGCTGGAATGCAAGTCACCGGC
    GCTGCTTACATTGAACCTTATGGTCAATTGTTTGAATATGGCTTTAATATTGATCATGAT
    GCTTGTATCCCTGGATTAACTAACATGGCATCTACGATGAAACAACATGGTAGCCTCGCT
    ATTATCCAGCTAGCGCATGCTGGTCGTTTTTCAAATCAAGCCATTTTAAATTTTGGAAAG
    GTGTATGGACCAAGCCCTATGACATTGCATTCACCGATTGAACACGATGTTATCGCCATG
    TCTCATGAGAAAATCAATAGCATCATTCAGCAGTATCGCGATGCTACATTACGCGCGATT
    AAAGCAGGTTTTGATGGTGTGGAAATTTCGATTGCACAACGTTTGCTGATTCAAACATTT
    TTCTCAACTTTTTCGAATAAACGTACAGACCATTACGGTGCCGACTCATTAAAAAATCGT
    GCAAGATTATGTTTAGAAGTTATGCGTGCGGTACAAGAAGTGATTGATAAAGAAGCACCT
    GACAATTTTATTTTAGGATTTCGAGCAACGCCAGAAGAAACCAGAGGCAGTGATTTAGGA
    TATACCATTGACGAGTTCAACCAATTAATCAATTGGGTCATGGACGTTTCAAACATCCAA
    TACTTAGCAATCGCTAGCTGGGGTCGACATATTTATCAAAATACGTCTCGTACACCAGGT
    GATCATTTCGGTCGACCAGTTAACCAAATTGTCTATGAACATTTAGCAGGTCGTATCCCT
    TTAATTGCAAGTGGCGGGATTAATTCTCCAGAGTCTGCTTTAGATGCTTTACAACATGCA
    GATATGGTCGGCATGTCTTCGCCTTTTGTCACAGAACCTGACTTTGTGCATAAACTTGCT
    GAACAGCGACCACATGATATCAATCTTGAATTTTCTATGGCAGATTTAGAAGATTTAGCC
    ATTCCACATGCTGCTTTTAAAGATATTGTTAAAATGATGGATTATGGGGAAGGTCTAAAG
    AAGCATACCCGTGACGCTTTACGTCAATTGGAACAGAATTATCATGACTCATCGTCTTAA
    60
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    840
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    960
    1020
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    1140
    1200

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS01975 [old locus tag: SACOL0392 ]
  • symbol: SACOL_RS01975
  • description: NADH-dependent flavin oxidoreductase
  • length: 399
  • theoretical pI: 6.56025
  • theoretical MW: 44691.3
  • GRAVY: -0.302506

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 148.2)
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 100)
    3-isopropylmalate dehydratase, small subunit (TIGR02087; HMM-score: 11.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherisopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 11.6)
  • TheSEED:
  • PFAM:
    TIM_barrel (CL0036) Oxidored_FMN; NADH:flavin oxidoreductase / NADH oxidase family (PF00724; HMM-score: 188.5)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 16.9)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 16.2)
    DHDPS; Dihydrodipicolinate synthetase family (PF00701; HMM-score: 12.8)
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.216
    • Ymax_pos: 23
    • Cmax: 0.136
    • Cmax_pos: 62
    • Smax: 0.593
    • Smax_pos: 17
    • Smean: 0.363
    • D: 0.274
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446208629 NCBI
  • RefSeq: WP_000286484 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDLAYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAIKAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS01975 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]