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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS02055 [old locus tag: SACOL0408 ]
  • pan locus tag?: SAUPAN001874000
  • symbol: SACOL_RS02055
  • pan gene symbol?:
  • synonym:
  • product: glyoxalase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS02055 [old locus tag: SACOL0408 ]
  • symbol: SACOL_RS02055
  • product: glyoxalase
  • replicon: chromosome
  • strand: +
  • coordinates: 415022..415882
  • length: 861
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (415022..415882) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    481
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    841
    ATGAATATAGTAGGGCATCATCACATATCCATGTATACAAAAGATGCAAAACGTAATAAG
    GATTTTTACACAAATGTCCTTGGATTACGATTAGTTGAAAAGTCGGTTAATCAAGACAAT
    CCTTCAATGTATCATTTGTTTTATGGGGACGAAGTAGGTACAGCCGGAACAATTTTAAGC
    TTTTTTGAAATTCCCAATGCGGGTCATAAGCAGCCAGGTACTGAAACGATTTATCGATTT
    TCATTATTAGTACCAAATCAAGCGGCACTTCATTATTTTGAAAAACGTCTTGAGAATAAT
    GGTATTAAGTCTGAACGTTTGTACTATCTTGGACAAGAAGGTGTTGTCTTTAAAGATGAA
    GACGACTTAGAAATCATATTGCTTGTTAATGATAGTTTTGAAGTACCACATCAATGGCAA
    CATAACGCTTATAGTGAAATACCTCAAGCATATCAAATTTTAGGAATAGGGCCAGTCGAA
    TTAAGAGTTAGAAATGCAGCGCGTACGGTAGAATTTTTGGAAAATGTCTTAGGTTATCGC
    AAAAGAGATAATAAATCATTCGATGTGCTGACATTAGCACCACAAGGTTTATATTCGGAT
    TTTGTAGTTATTGAGCAACAGGGACAACGTGAAAGACCTGGACGAGGTTATATCCATCAT
    ATTGCAGTTAATACACCACAAATGAGTGACTTAGATGCAATTTACAAGAAATTACAACAA
    CAACCACAAAGTAATTCAGGTATAATTGATCGCTATTTCTTTAAATCATTATACTATCGC
    CATAATTCAATTATGTATGAATTTGCGACTGAAGCGCCTGGATTTACTATTGATACACCT
    GTTGAACAATTAGGAAGTTAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS02055 [old locus tag: SACOL0408 ]
  • symbol: SACOL_RS02055
  • description: glyoxalase
  • length: 286
  • theoretical pI: 6.28804
  • theoretical MW: 32944.8
  • GRAVY: -0.474476

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismEnergy metabolismOthercatechol 2,3 dioxygenase (TIGR03211; EC 1.13.11.2; HMM-score: 29)
    MetabolismEnergy metabolismOtherlactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 25.7)
    MetabolismCentral intermediary metabolismAmino sugarslactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 25.7)
    3,4-dihydroxyphenylacetate 2,3-dioxygenase (TIGR02295; EC 1.13.11.15; HMM-score: 20.7)
    MetabolismEnergy metabolismOther2,3-dihydroxybiphenyl 1,2-dioxygenase (TIGR03213; EC 1.13.11.39; HMM-score: 19.8)
    methylmalonyl-CoA epimerase (TIGR03081; EC 5.1.99.1; HMM-score: 15.9)
  • TheSEED:
  • PFAM:
    Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 74.2)
    Glyoxalase_4; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF13669; HMM-score: 19.3)
    CppA_N; CppA N-terminal (PF14506; HMM-score: 18)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.128
    • Ymax_pos: 17
    • Cmax: 0.117
    • Cmax_pos: 45
    • Smax: 0.188
    • Smax_pos: 6
    • Smean: 0.144
    • D: 0.134
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446950964 NCBI
  • RefSeq: WP_001028220 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMYHLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGIKSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVELRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYIHHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFTIDTPVEQLGS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS02055 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]