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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04295 [old locus tag: SACOL0834 ]
  • pan locus tag?: SAUPAN002697000
  • symbol: SACOL_RS04295
  • pan gene symbol?:
  • synonym:
  • product: epimerase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS04295 [old locus tag: SACOL0834 ]
  • symbol: SACOL_RS04295
  • product: epimerase
  • replicon: chromosome
  • strand: -
  • coordinates: 860781..861683
  • length: 903
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (860781..861683) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACAATACTTAATTACTGGTGGGACTGGTATGGTTGGATCTCAATTAGTTAATGAA
    ATTAAAAAATCAGATTCACATATCACGATATTAACGCGACACGACCAAATTTCAAATGAT
    AAGAAAATTTCATATGTCAACTGGGCTAAATCTGGGTGGGAACACAAAGTTCCTCAAAAT
    ATCGATGTGGTCATCAACTTAGCAGGTGCTACATTGAATAAACGATGGACACCGGAATAT
    AAACAAACGCTAATGCTAAGTAGAATTCAATCTACGCAAGCTTTATATGAATTGTTTAAA
    TCACGTAATAAAGCACCTAAAGCTTTATTTAATGCTAGTGCCACTGGTTACTATCCTCCT
    GATTTATTTATGAGTTACACAGAAGTTTATAAAACTTTACCTTTTGATTTCTTATCAGAT
    ATTGTGTATCAATGGGAACGTTTCGCACAACAATTTGAACAATTAGGTACACGAGTTGTC
    ATCGGTCGTTTCGGTATAATTTTATCGAATGAAGGCGGTGCGTTACAAACAATGAAACTA
    CCATACGAATATTACATTGGTGGTAAATTAGGTTCTGGTCAACAATGGTATTCATGGATT
    CATATCAATGATTTAATTCAAGCTATTTTATTTTTAATAAATAACGAGTCAGCTAGTGGT
    CCGTTTAATTTAACTGCACCTATACCTGAACGTCAAAATTTATTTGGCTACACTTTAGCA
    AGAGCTATGCATAAGCCTCATGAAACTTGGGCACCAAGTCTTGCAATGCGTCTCATACTT
    GGTCAAATGTCAACAGTAGTATTGGATACTCAAAAAGTATTACCTAATAAAATTCAAGCA
    TTGGGATTCCAATTTAAATATAGTAATTTAAAAATGGCACTTGAAGATTTAATTAAAGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    903

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS04295 [old locus tag: SACOL0834 ]
  • symbol: SACOL_RS04295
  • description: epimerase
  • length: 300
  • theoretical pI: 9.72292
  • theoretical MW: 34227.2
  • GRAVY: -0.227

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Hypothetical proteinsConservedTIGR01777 family protein (TIGR01777; HMM-score: 361.9)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 29.7)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesdTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 20.2)
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 15.5)
    MetabolismEnergy metabolismSugarsUDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 14.3)
    thioester reductase domain (TIGR01746; HMM-score: 12.7)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 67.6)
    no clan definedDUF1731; Domain of unknown function (DUF1731) (PF08338; HMM-score: 62)
    NADP_Rossmann (CL0063) NAD_binding_4; Male sterility protein (PF07993; HMM-score: 24.9)
    GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 20.7)
    KR; KR domain (PF08659; HMM-score: 18)
    RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 15.5)
    Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 14.4)
    Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 14.3)
    NmrA; NmrA-like family (PF05368; HMM-score: 12.9)
    NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.16
    • Ymax_pos: 24
    • Cmax: 0.135
    • Cmax_pos: 24
    • Smax: 0.315
    • Smax_pos: 1
    • Smean: 0.173
    • D: 0.165
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446739039 NCBI
  • RefSeq: WP_000816295 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKISYVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSRNKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVVIGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESASGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPNKIQALGFQFKYSNLKMALEDLIKE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS04295 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]