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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04880 [old locus tag: SACOL0952 ]
  • pan locus tag?: SAUPAN003050000
  • symbol: SACOL_RS04880
  • pan gene symbol?: mnhD
  • synonym:
  • product: Na+/H+ antiporter subunit D

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS04880 [old locus tag: SACOL0952 ]
  • symbol: SACOL_RS04880
  • product: Na+/H+ antiporter subunit D
  • replicon: chromosome
  • strand: -
  • coordinates: 952912..954408
  • length: 1497
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (952912..954408) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGATTGAAAGTAATATGCTTGTGTTAACGCTAGTTATTCCAGTAATTACAGCCATATTA
    CTTGTCTTTATTGGAAAGCGTCCTATTATAAAGCGTTATGTCGCACTAGGCGGTACATTA
    TTAACCTTGGTCGCGGCAATTATCAATTTAGCAAATGTTGTTAAACATGGACCAATACGC
    GTAGAGCTAGGTTCATGGAAAGCACCATACAGTATTGTCTTTGTATTAGACATATTCAGT
    GCCCTACTAATAATTACTAGTATTATTATTACAGCTATTGTGATTTTATATTCAAGCCAA
    ACAATTGGTATTGAACGTGAAAGATATTATTATTATTTTTCAGTACTATTTATGTTAATA
    GGTATCATTGGTGCATTCACAACTGGTGACATTTTCAACCTATTCGTATTTTTCGAAGTA
    TTTTTAATGTCATCTTATTTCTTATTAGTAATTGGTTCTACAAAAATTCAACTACAAGAA
    ACAATAAAATATGTTCTTGTTAACGTTGTATCGTCATCCTTCTTCGTAATGGGTGTAGCG
    ATTTTATATTCAGTAGTAGGAACATTGAATCTAGCAGATATAAGCAATAAATTAGCTAAC
    CTTTCAGCGCATGACAGTGGCTTAGTCAATATCGTTTTCATACTATTTATCTTTGTTTTT
    GCTACAAAAGCTGGCGTATTCCCAATGTTTGTTTGGTTACCTAGTGCATACTATGCACCA
    CCAATTCCAATCATTGCATTCTTTGGTGCTTTGTTAACAAAAGTTGGTGTTTATGCAATC
    GCTAGAACATTGAGCCTATTCTTTAGTGATAATGTAAGCTTTTCACATTATGTCATTTTA
    TTCTTAGCACTATTAACGATCATCTTTGGTTGTGTCGGTGCTGTTGCATATGCCAACATT
    AAAAAAATCATCCTATACAATGTCATGATTGCTGTAGGTGTGATCTTAGTCGGCGTTGCA
    ATGATGACAGAGTCAGGTATGATTGGGGCTATTTACTATACATTGCACGACATGTTAGTA
    AAACTTGCATTATTCTTGCTTATCGGAATTATGATTAAAATTACAGGCACTGCTGATTTA
    AGACAATTTGGTGGCTTAATTAAACGATATCCTGTACTTGGTTGGTCATTCTTTATCGCA
    GCATTAAGTTTAGCTGGCATTCCCCCACTCAGTGGCTTTTATGGAAAATTTTTTATTGTC
    CAATCAACATTTGAAAGAGGATTTTATTTAAGTGGTGTCATCGTCTTATTATCAAGTTTA
    GTCGTATTATATTCTGTCATTCGCATATTCCTACAAGGCTTTTTCGGTCAACCAAAAGGA
    TATGATCTTAATAACAAAGTCGACGTCAAATATTTAACTACGATTGCAATCGTTGCAGTT
    GTGATAACTGTTTTATATGGATTGTCAGCAGATTACTTATATCCAATGGTAAAAGCTGGT
    GCAGAAACATTCTACAATCCATCAACCTACGTTAAAGCTGTATTAGGAGGTAAATAA
    60
    120
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    1020
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    1140
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    1260
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    1380
    1440
    1497

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS04880 [old locus tag: SACOL0952 ]
  • symbol: SACOL_RS04880
  • description: Na+/H+ antiporter subunit D
  • length: 498
  • theoretical pI: 9.71816
  • theoretical MW: 54679.4
  • GRAVY: 1.12791

Function[edit source | edit]

  • reaction:
    EC 1.6.5.3?  ExPASy
    NADH:ubiquinone reductase (H+-translocating)NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out)
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmulticomponent K+:H+antiporter (TIGR00944; HMM-score: 762.6)
    MetabolismEnergy metabolismElectron transportproton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 190.9)
    MetabolismEnergy metabolismElectron transportproton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 159.3)
    MetabolismEnergy metabolismElectron transportproton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 111.4)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmonovalent cation:proton antiporter (TIGR00940; HMM-score: 92.4)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 73.7)
  • TheSEED:
  • PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 174.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.147
    • Ymax_pos: 11
    • Cmax: 0.125
    • Cmax_pos: 52
    • Smax: 0.252
    • Smax_pos: 7
    • Smean: 0.227
    • D: 0.178
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit source | edit]

  • GI: 446495217 NCBI
  • RefSeq: WP_000573071 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSSQTIGIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQETIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS04880 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]