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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05365 [old locus tag: SACOL1052 ]
  • pan locus tag?: SAUPAN003246000
  • symbol: SACOL_RS05365
  • pan gene symbol?: menD
  • synonym:
  • product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS05365 [old locus tag: SACOL1052 ]
  • symbol: SACOL_RS05365
  • product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1057870..1059543
  • length: 1674
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1057870..1059543) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    1561
    1621
    ATGGGAAATCATAAAGCAGCTTTAACGAAGCAAGTTTTTACATTTGCATCTGAGTTATAT
    GCGTACGGCGTAAGGGAAGTAGTTATCAGTCCGGGATCACGCTCAACGCCACTTGCACTT
    GCATTTGAAGCACATCCAAATATTAAAACATGGATACACCCCGATGAGCGAAGTGCAGCG
    TTTTTTGCAGTTGGGTTAATTAAAGGCAGTGAAAGACCTGTCGCTATATTATGTACGTCA
    GGTACAGCAGCAGCGAATTATACGCCTGCAATTGCTGAAAGCCAAATTAGTAGAATTCCT
    TTAATCGTTTTAACAAGTGACCGTCCGCATGAATTAAGAAGTGTAGGCGCACCACAAGCG
    ATTAATCAAGTAAATATGTTTAATAATTATGTAAGTTATGAGTTCGATATGCCTATTGCG
    GATGATAGTAAAGAGACCATTGATGCAATTTACTATCAAATGCAAATTGCTAGTCAATAT
    TTATATGGACCACATAAAGGGCCAATTCATTTTAACTTGCCATTTAGAGATCCGTTAACA
    CCTGATTTGAATGCAACAGAATTGTTAACTTCTGAGATGAAGATTTTACCGCACTATCAA
    AAAAGTATAGATGCATCGGCATTAAGACACATTTTAAATAAGAAAAAAGGTTTAATTATT
    GTAGGGGATATGCAGCACCAAGAAGTTGATCAAATACTAACGTATTCAACGATATATGAT
    TTGCCTATTTTAGCTGATCCTTTAAGTCATTTAAGAAAATTTGATCATCCGAATGTTATC
    TGTACATATGATTTGCTGTTTAGAAGCGGCTTAGACTTAAATGTGGATTTCGTAATTCGT
    GTTGGGAAACCAGTGATTTCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTT
    CAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCA
    TATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGC
    GTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGT
    TATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTGGTGAATTGATTAAGAAAACATCT
    GAAAAAGATGCATTATTTATTAGTAATAGTATGCCTATCAGAGATGTAGATAACTTGTTA
    TTGAATAAAAATATAGATGTCTATGCGAATCGTGGTGCGAATGGTATTGATGGTATCGTT
    TCAACTGCACTGGGTATGGCTGTGCATAAACGAATAACATTATTGATAGGTGATTTATCA
    TTTTATCATGATATGAATGGACTATTAATGTCAAAATTAAATAATATTCAGATGAATATT
    GTATTATTGAACAACGATGGTGGCGGTATTTTTTCATATTTACCACAAAAAGAAAGTGCA
    ACTGACTATTTTGAACGGTTGTTTGGCACACCGACGGGATTGGATTTCGAGTATACAGCT
    AAGTTATATCAATTCGATTTTAAACGTTTTAACAGTGTTTCAGAATTTAAAAATGCCACA
    TTGTTATCTGAAACTTCGACGATTTATGAATTGATAACGAATCGCGAAGATAACTTTAAA
    CAGCATCAAATTTTATACCAGAAATTGAGTGAAATGATTCATGACACATTATAA
    60
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    1020
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    1560
    1620
    1674

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS05365 [old locus tag: SACOL1052 ]
  • symbol: SACOL_RS05365
  • description: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase
  • length: 557
  • theoretical pI: 6.24326
  • theoretical MW: 63077.7
  • GRAVY: -0.187612

Function[edit source | edit]

  • reaction:
    EC 4.1.1.71?  ExPASy
    2-oxoglutarate decarboxylase2-oxoglutarate = succinate semialdehyde + CO2
    EC 2.2.1.9?  ExPASy
    2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthaseIsochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 428.2)
    MetabolismAmino acid biosynthesisPyruvate familyacetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 36.3)
    MetabolismEnergy metabolismSugars3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 31.4)
    MetabolismEnergy metabolismFermentationacetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 19.3)
    MetabolismCentral intermediary metabolismOthersulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 16.3)
    2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 14.5)
  • TheSEED:
  • PFAM:
    THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 111.4)
    FAD_DHS (CL0085) TPP_enzyme_M_2; Middle domain of thiamine pyrophosphate (PF16582; HMM-score: 46.1)
    THDP-binding (CL0254) TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 23.6)
    NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+, thiamine diphosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.168
    • Ymax_pos: 42
    • Cmax: 0.151
    • Cmax_pos: 42
    • Smax: 0.361
    • Smax_pos: 1
    • Smean: 0.15
    • D: 0.161
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446448832 NCBI
  • RefSeq: WP_000526687 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQYLYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLMEDTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS05365 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]