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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07300 [old locus tag: SACOL1433 ]
  • pan locus tag?: SAUPAN003811000
  • symbol: SACOL_RS07300
  • pan gene symbol?: dapL
  • synonym:
  • product: hydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS07300 [old locus tag: SACOL1433 ]
  • symbol: SACOL_RS07300
  • product: hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1445356..1446507
  • length: 1152
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1445356..1446507) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATGAATTAGAATTTGTTACGAAACATCGCCGTCATTTACATCAACATCCTGAATTA
    AGCTTACATGAATTTGAAACAACTGCTTATATTAAAGCGTTTTTAGATAGTTTAAATATT
    AAATACGATTGCCCATTGGAAACTGGCGTCATTGCATACTTAGAAGGTAATGGCTCACAT
    ACGATAGCGTATAGAGCTGATATTGATGCGTTACCTATTTTAGAGGAAAATGATGTGCCT
    TATCGCAGTCAATCTGATCATGTGATGCATGCTTGTGGACATGATGGTCATACAACTGCA
    TTAATGCTTTTTGTACAACGTTGCAAAGACATGCAAGATGCAGGTCAATTACCGCAAAAT
    GTCGTTTTCATTTTCCAACCTGCAGAAGAAACTGGTGGCGGTGCAAATCGATTAATAAAA
    GCCGGTGCCTTTGATAAGTATCCAATTGAAGCGGTATTTGGTATTCATGTTAACCCATTT
    GCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTAT
    CGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGT
    GGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAAC
    GGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACT
    GTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACA
    ATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAT
    TGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGAAATTACGTACT
    CAAATAGAGGACGCCTTAATAAAAGCTGATTTAAATGTCTATGACAAACCAACGCCATTC
    TTATTTGGGGAAGATTTTAGTTTTTATGGTCAACAACTAGCTCCAGCTTACTTTGTTTTT
    ATAGGAACACGAAATGAAGATAAAGGTTTTGTAACTGGTTTGCACACATCACATTTAAAT
    TTTGATGAAAAAGTGTTAATAAACGTGATTAATTTTTACGAAAATTTATTAAATAATTAC
    AAAGAGGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1152

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS07300 [old locus tag: SACOL1433 ]
  • symbol: SACOL_RS07300
  • description: hydrolase
  • length: 383
  • theoretical pI: 5.35281
  • theoretical MW: 43157.4
  • GRAVY: -0.251436

Function[edit source | edit]

  • reaction:
    EC 3.-.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesamidohydrolase (TIGR01891; HMM-score: 279.5)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 20.6)
    MetabolismAmino acid biosynthesisGlutamate familyacetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 20.4)
  • TheSEED:
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 117.6)
    no clan definedM20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 22.7)
    DUF1310; Protein of unknown function (DUF1310) (PF07006; HMM-score: 13.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.112
    • Ymax_pos: 12
    • Cmax: 0.121
    • Cmax_pos: 66
    • Smax: 0.144
    • Smax_pos: 1
    • Smean: 0.122
    • D: 0.116
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446926536 NCBI
  • RefSeq: WP_001003792 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDCPLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALMLFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPFADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFHLNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLLFNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLAPAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVINFYENLLNNYKEV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS07300 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]