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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07615 [old locus tag: SACOL1495 ]
  • pan locus tag?: SAUPAN003915000
  • symbol: SACOL_RS07615
  • pan gene symbol?: dinG
  • synonym:
  • product: ATP-dependent helicase DinG

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS07615 [old locus tag: SACOL1495 ]
  • symbol: SACOL_RS07615
  • product: ATP-dependent helicase DinG
  • replicon: chromosome
  • strand: -
  • coordinates: 1534589..1537282
  • length: 2694
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1534589..1537282) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    841
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    1021
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    ATGGGTATGGCAACCTATGCCGTTGTGGATTTGGAAACAACAGGCAACCAATTAGATTTT
    GACGATATCATTCAAATTGGTATAACGTTTGTGCGCAATAACCAAATTATTGATACATAT
    CATTCTATGATTAGAACAAATTTAGAGATTCCGCCGTTCATTCAAGCGTTAACATCCATT
    GAAGAAAATATGCTACAACAAGCGCCATATTTTAATCAAGTTGCACAAGAAATCTACGAT
    AAGATAAAAGATTGCATTTTTGTAGCTCATAATGTAGATTTTGATTTGAATTTTATTAAA
    AAGGCATTTAAAGATTGTAATATTCAATATCGTCCCAAAAAAGTTATAGATACACTAGAA
    ATCTTTAAAATAGCTTTTCCTACAGATAAAAGCTATCAATTAAGTGAATTAGCAGAGGCA
    CATGGTATTACCTTAGCAAATGCCCATCGAGCTGACGAAGATGCCGCTACTACTGCTAAA
    TTGATGATATTAGCTTTTGAAAAATTTGAAAAGTTACCGTTAGATACTTTGAAGCAATTA
    TATTATTTAAGTAAACAGTTGAAATACGATTTGTATGATATTTTCTTTGAGATGGTTAGG
    CAATATGATGCAAAGCCATTGGATAAATCGTACGAAAAGTTCGAACAGATTATCTATCGT
    AAGCAAGTTGATTTTAAAAAGCCGACAACAAATTATAATGGCAGCTTGAAGTCTTTATAT
    AGCAAAGCTGTAGATCAACTTGGCTTAACATATCGACCGCAACAATTATATTTAGCGGAA
    ACTATATTAGATCAGCTCATGCATAGTGAAAAAGCAATGATTGAAGCATCACTAGGCAGT
    GGTAAATCATTAGCATATTTACTTGCGGCCTTGATGTATAATATTGAAACTGGGAAGCAT
    GTCATGATTTCTACCAATACTAAATTACTGCAAAGTCAATTGTTAGAAAAAGATATTCCA
    GCAATGAATGAAGCGTTGAATTTTAAAATTAATGCACTATTGATTAAAAGTAAAAGTGAT
    TACATTTCATTGGGGCTTATTAGTCAAATTTTAAAAGATGACACAAGTAATTACGAAGTG
    AATATCTTGAAAATGCAATTGCTGATTTGGATTACAGAGACGCCGTCCGGAGATATACAG
    GAATTAAATTTAAAAGGTGGTCAAAAGATGTATTTTGACCAGAAAATTGAAACATATGTA
    CCGGCACGTCACGATGTTCATTATTATAATTTTATTAAGCGAAATGCACAGAACATTCAA
    ATTGGTATTACAAATCATGCACATTTAATTCATTCTGATGTTGAAAATTCAATATATCAA
    TTGTTTGATGATTGTATAGTGGATGAAGCACATCGCTTACCAGACTATGCCTTAAATCAA
    GTGACAAATGAGTTGAGTTATGCTGATATTAAATATCAGCTTGGTTTGATTGGTAAAAAC
    GAAAATGAAAAATTGTTAAAGGCAATTGATCAACTGGAAAAGCAGCGAATTTTGGAAAAA
    TTAGATATTGCACCAATTGATATTTTTGGCTTGAAGGCTAGTATGAATGAGATTCATGAG
    TTAAATGAGCAATTGTTCTCAACGATATTTACGATTATCAATGATTCTGATGTTTATGAT
    GATGATATTCATCGCTTCCATAATGTATTTACTTTTGAAACAAAAGACATTTTGAAGGAT
    TTACATGCGATTATCGATAAGTTAAATAAAACGCTAGAAATATTCAATGGCATTAGTCAT
    AAAACAGTTAAATCATTACGGAAACAGTTGTTGTATTTAAAAGATAAATTTAAAAATATC
    GAACAAAGTTTAAAAGCTGGACATACTAGTTTCATTTCGATTAAAAATTTATCTCAAAAA
    TCAACAATACGTTTATATGTGAAAGATTACGCTGTAAAAGATGTATTAACGAAACAAGTA
    TTGGAAAAATTTAAATCACTCATTTTTATATCTGGTACTTTAAAATTTAATCATTCGTTT
    GAAGCTTTTAAACAGTTATTCAACAAAGATGTTCACTTTAATACATTTGAAGTTAACACG
    TCGTTACAAAGTGCAAAAAATACAAGTGTATTTATACCGAGTGATGTAGCATCTTATCAA
    TATAAAAATATAGATGAGTATGTAGCATCGATTGTAAGCTATATTATTGAATATACGACT
    ATAACGTCATCAAAATGTTTAGTCTTATTTACGAGTTATAAAATGATGCATATGGTACAA
    GATATGCTAAATGAATTGCCAGAATTTGAAGATTATGTTGTATTAACACAACAGCAGAAC
    CAAAATTATAAAATAGTACAACAGTTTAATAATTTTGATAAGGCCATACTGCTTGGTACG
    TCAACATTTTTTGAAGGTTTTGATTTTCAAGCAAATGGTATTAAATGTGTGATGATAGCG
    AAGTTACCGTTTATGAACAAGCATAATGCAAAATATTGGCTTATGGATTCTGAATTCACT
    TCAACTTTCAAGGAATATGTATTACCTGATGCAGTTACACGTTTTAGACAAGGACTTGGA
    AGATTAATTAGAAATGAAAATGATCGCGGCATAATAGTTTCATTTGATGATCGACTCATA
    AACAGTAATTATAAAAACTTTTTTGAACAAACACTTGAAAATTATCGTCAGAAAAAAGGC
    GACATTCAACAGTTTGGAAAATTATTAAGACAAATTCAAAAAAAGAAAAAGTGA
    60
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    2694

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS07615 [old locus tag: SACOL1495 ]
  • symbol: SACOL_RS07615
  • description: ATP-dependent helicase DinG
  • length: 897
  • theoretical pI: 6.83206
  • theoretical MW: 104217
  • GRAVY: -0.297101

Function[edit source | edit]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicaseATP + H2O = ADP + phosphate
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairputative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 1269.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 139.1)
    Genetic information processingDNA metabolismDegradation of DNAexonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 94.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 60.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA repair helicase (rad3) (TIGR00604; EC 3.6.4.-; HMM-score: 57.1)
    CRISPR type AFERR-associated DEAD/DEAH-box helicase Csf4 (TIGR03117; HMM-score: 47.5)
    Genetic information processingTranscriptionRNA processingribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 17.9)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C_2; Helicase C-terminal domain (PF13307; HMM-score: 112.3)
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 91.3)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 22.8)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 20.7)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 18.7)
    no clan definedHsbA; Hydrophobic surface binding protein A (PF12296; HMM-score: 14.6)
    DUF615; Protein of unknown function (DUF615) (PF04751; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.098
    • Ymax_pos: 68
    • Cmax: 0.131
    • Cmax_pos: 57
    • Smax: 0.119
    • Smax_pos: 16
    • Smean: 0.069
    • D: 0.087
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446447223 NCBI
  • RefSeq: WP_000525078 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMIRTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKAFKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAKLMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQIIYRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEASLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALLIKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYFDQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEAHRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDIFGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIIDKLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIRLYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSLQSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQDMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVMIAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFDDRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS07615 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]