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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08600 [old locus tag: SACOL1686 ]
  • pan locus tag?: SAUPAN004236000
  • symbol: SACOL_RS08600
  • pan gene symbol?: hisS
  • synonym:
  • product: histidine--tRNA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08600 [old locus tag: SACOL1686 ]
  • symbol: SACOL_RS08600
  • product: histidine--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1715050..1716312
  • length: 1263
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1715050..1716312) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATTAAAATACCTAGAGGGACGCAGGATATTTTACCTGAAGATTCAAAGAAATGGCGT
    TACATTGAAAATCAATTAGATGAATTAATGACATTTTATAATTATAAAGAAATAAGAACA
    CCAATTTTTGAAAGTACAGATCTTTTTGCAAGAGGTGTTGGTGATTCAACCGATGTCGTA
    CAAAAAGAAATGTATACATTTAAAGATAAAGGCGATAGAAGTATTACATTAAGACCTGAG
    GGAACAGCTGCAGTTGTGCGTTCATATATTGAACATAAAATGCAAGGTAATCCAAACCAA
    CCAATTAAACTTTATTACAATGGACCGATGTTTAGATATGAACGTAAGCAAAAAGGACGC
    TATCGTCAATTTAATCAATTTGGTGTAGAAGCTATTGGTGCTGAAAATCCTAGCGTAGAT
    GCAGAAGTATTAGCTATGGTTATGCATATTTATCAATCATTTGGATTAAAACATTTAAAG
    CTTGTTATTAATAGTGTAGGGGATATGGCGTCTCGAAAAGAATATAACGAAGCGTTAGTG
    AAACACTTTGAACCAGTAATTCATGAATTTTGTTCAGATTGTCAATCACGTTTGCATACA
    AATCCGATGCGAATTTTGGATTGTAAAGTAGACCGTGATAAAGAAGCGATTAAGACTGCA
    CCTAGAATCACTGATTTCTTAAATGAGGAATCTAAGGCATATTATGAACAAGTAAAAGCT
    TATTTAGATGATTTAGGTATTCCATATATTGAAGATCCTAACTTAGTTCGTGGATTGGAT
    TATTATACACATACAGCATTTGAATTAATGATGGATAACCCTAACTATGATGGTGCCATT
    ACAACGCTTTGTGGTGGTGGCCGTTATAATGGTTTATTAGAATTGCTAGATGGTCCAAGT
    GAAACAGGTATTGGTTTTGCGCTAAGTATAGAACGATTATTGCTTGCACTTGAAGAAGAA
    GGTATCGAATTAGATATTGAAGAAAACTTAGATTTATTCATTGTTACAATGGGTGATCAA
    GCAGATCGATATGCTGTGAAGCTATTAAATCATTTGAGACATAATGGTATTAAAGCAGAT
    AAAGACTATTTACAGCGTAAAATTAAAGGACAAATGAAACAAGCAGACCGTTTAGGTGCC
    AAGTTTACAATCGTTATTGGTGATCAAGAATTAGAAAATAATAAAATCGATGTTAAAAAT
    ATGACAACTGGTGAATCTGAAACAATTGAATTAGACGCATTAGTCGAATATTTTAAGAAG
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08600 [old locus tag: SACOL1686 ]
  • symbol: SACOL_RS08600
  • description: histidine--tRNA ligase
  • length: 420
  • theoretical pI: 5.01807
  • theoretical MW: 48282.6
  • GRAVY: -0.519048

Function[edit source | edit]

  • reaction:
    EC 6.1.1.21?  ExPASy
    Histidine--tRNA ligaseATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationhistidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 510.5)
    MetabolismAmino acid biosynthesisHistidine familyATP phosphoribosyltransferase, regulatory subunit (TIGR00443; HMM-score: 169.6)
    Genetic information processingProtein synthesistRNA aminoacylationthreonine--tRNA ligase (TIGR00418; EC 6.1.1.3; HMM-score: 57.1)
    Genetic information processingProtein synthesistRNA aminoacylationproline--tRNA ligase (TIGR00409; EC 6.1.1.15; HMM-score: 31.9)
    Genetic information processingProtein synthesistRNA aminoacylationproline--tRNA ligase (TIGR00408; EC 6.1.1.15; HMM-score: 24.6)
    Genetic information processingProtein synthesistRNA aminoacylationpyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 19.6)
    Genetic information processingProtein synthesistRNA aminoacylationphenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 14)
    Genetic information processingProtein synthesistRNA aminoacylationglycine--tRNA ligase (TIGR00389; EC 6.1.1.14; HMM-score: 12.6)
  • TheSEED:
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_His; Histidyl-tRNA synthetase (PF13393; HMM-score: 162.3)
    tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 72.7)
    IIaaRS-ABD (CL0458) HGTP_anticodon; Anticodon binding domain (PF03129; HMM-score: 70.2)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 15.8)
    IIaaRS-ABD (CL0458) HGTP_anticodon2; Anticodon binding domain of tRNAs (PF12745; HMM-score: 15.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.097
    • Ymax_pos: 70
    • Cmax: 0.103
    • Cmax_pos: 19
    • Smax: 0.111
    • Smax_pos: 68
    • Smean: 0.073
    • D: 0.088
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446513480 NCBI
  • RefSeq: WP_000590826 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFESTDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPIKLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLKLVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIKTAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNYDGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFIVTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELENNKIDVKNMTTGESETIELDALVEYFKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08600 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]