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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08875 [old locus tag: SACOL1736 ]
  • pan locus tag?: SAUPAN004312000
  • symbol: SACOL_RS08875
  • pan gene symbol?: mutM
  • synonym:
  • product: DNA-formamidopyrimidine glycosylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08875 [old locus tag: SACOL1736 ]
  • symbol: SACOL_RS08875
  • product: DNA-formamidopyrimidine glycosylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1766901..1767773
  • length: 873
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1766901..1767773) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    ATGCCCGAATTACCAGAAGTAGAACATGTAAAAAGAGGAATTGAACCCTACGTTATAAAT
    CAAAAAATTGAACACGTTATATTTTCGGATAAAGTAATCGAAGGTAAAGCTCAAGGCAAG
    GAAACAATTATAAAAGGAATTGAATTAGATACTTTTAAAACTTTATCGGAAGGCTATACC
    ATTACAAATGTAGAACGAAGAAGTAAATATATCGTTTTTCAGCTAGATAATAAACGAGAA
    CAACGTACATTAATTAGTCATTTAGGTATGGCGGGCGGTTTTTTCATTGTAGATGAACTT
    GAAGATATTATGATACCTAATTATCGTAAGCATTGGCATGTTATTTTTGAGTTGTCAAAT
    GACAAAAAGTTAATTTATTCAGATATACGTCGTTTTGGAGAAATTAGAAATGTTGCGTCT
    GTAGCATCTTATCCTTCATTTTTAGAAATAGCGCCTGAACCTTTTTCAAATGAAGCATTG
    ACGTATTATTTAAATCGAATTCATCAACAGTCTAATAAGAATAAACCAATCAAACAAGTG
    ATTTTGGATCATAAAGTAATCGCAGGTTGTGGAAATATTTACGCATGTGAAGCGTTGTTT
    CGTGCAGGTGTTTTACCAGATAAAAAAGTAAAAGACTTAACACACCAACAACAAGAAATG
    GTTTTTTATTATGTTAGAGAAGTACTAGAAGAAGGTATTAAATATGGCGGTACAAGTATT
    TCAGATTATCGTCATGCTGATGGAAAAACTGGTGAGATGCAATTGCATCTTAATGTATAT
    AAGCAGCCGGTATGCAAAGTGTGTGGAAGTCAAATTGAAACAAAAATCATCGCGACAAGA
    AATAGTCATTATTGTCCAGTATGTCAAAAATAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08875 [old locus tag: SACOL1736 ]
  • symbol: SACOL_RS08875
  • description: DNA-formamidopyrimidine glycosylase
  • length: 290
  • theoretical pI: 8.12881
  • theoretical MW: 33411.2
  • GRAVY: -0.39069

Function[edit source | edit]

  • reaction:
    EC 4.2.99.18?  ExPASy
    DNA-(apurinic or apyrimidinic site) lyaseThe C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
    EC 3.2.2.23?  ExPASy
    DNA-formamidopyrimidine glycosylaseHydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA-formamidopyrimidine glycosylase (TIGR00577; EC 3.2.2.23; HMM-score: 235.5)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal protein uS13 (TIGR03631; HMM-score: 15.1)
  • TheSEED:
  • PFAM:
    no clan definedFapy_DNA_glyco; Formamidopyrimidine-DNA glycosylase N-terminal domain (PF01149; HMM-score: 99.2)
    H2TH (CL0303) H2TH; Formamidopyrimidine-DNA glycosylase H2TH domain (PF06831; HMM-score: 70.1)
    Zn_Beta_Ribbon (CL0167) zf-FPG_IleRS; Zinc finger found in FPG and IleRS (PF06827; HMM-score: 24.7)
    H2TH (CL0303) FbpA; Fibronectin-binding protein A N-terminus (FbpA) (PF05833; HMM-score: 16.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 40
    • Cmax: 0.122
    • Cmax_pos: 40
    • Smax: 0.118
    • Smax_pos: 34
    • Smean: 0.077
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447037196 NCBI
  • RefSeq: WP_001114452 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETIIKGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELEDIMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEALTYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQEMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKIIATRNSHYCPVCQK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08875 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]