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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08880 [old locus tag: SACOL1737 ]
  • pan locus tag?: SAUPAN004313000
  • symbol: SACOL_RS08880
  • pan gene symbol?: polA
  • synonym:
  • product: DNA polymerase I

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08880 [old locus tag: SACOL1737 ]
  • symbol: SACOL_RS08880
  • product: DNA polymerase I
  • replicon: chromosome
  • strand: -
  • coordinates: 1767789..1770419
  • length: 2631
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1767789..1770419) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1981
    2041
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    2581
    GTGAATAAATTAGTATTAATCGATGGTAATAGCTTAAGTTTTAGAGCTTTTTATGCATTA
    CCTTTGTTATCAAACAAAGCAGGCATTCATACCAATGCAGTATATGGTTTTGCAATGTTA
    CTAGAGAAGATTTTAAAAGAAGAAAAGCCAAATCATTTTTTAGTAGCGTTTGATGCAGGT
    AAAACGACTTTCAGACATGAAAAATATAGTGAATATAAAGGTGGACGCCAGAAAACGCCG
    CCTGAACTAAGTGAGCAATTTCCTTATATTCGCCAATTATTAGATGCTTATCATATTAAA
    CGTTATGAATTAGATAACTATGAGGCAGATGATATTATCGGAACTTTAAGTAAAGAAGCA
    GATAAAGCGGGCTTTCAGACAATTATTATTACGGGAGACCGAGATTTAACACAACTTGCA
    ACGGATAATGTAACCATTTATTACACTAAAAAAGGTGTTACTGATGTTGATCATTATACA
    CCTGACTTTATTGCGGAAAAATACAACGGGTTAACACCTAATCAAATTATTGATATGAAA
    GGATTAATGGGAGATACTTCTGATAATATACCAGGTGTTGCAGGTGTTGGAGAGAAAACA
    GCAATAAAATTGCTAAACCAATTTGACACAGTAGAAGGTGTCTATGAACATTTAGATGAA
    ATTTCAGGTAAAAAGTTAAAAGAAAAACTTCAAAATAGCAAAGAAGATGCATTAATGAGT
    AAAGAATTAGCAACGATTAATGTAGATAGTCCGATTGAAGTAAAACTTGAAGATACGTTA
    ATGACTCATCAAGATGAACAACAAGAAAAAATCGAATTGTTTAAGAAGTTAGAATTCAAA
    CAATTGTTGGCTGACATTGATCAATCAGCAAGCGTTGAAGATGCAATAGAAAAGACATTT
    GAAATTGAAACGTCTTTTGATAATATTGATTTTACTTCATTGAAAGAAGCGGCCATCCAT
    TTCGAATTAGACGGTGGTAACTATTTGCGAAATAATATTTTAAAATTCTCTTTATTTACA
    GGTGAGAAACATATTGTAATTAATGCGGATGACATAAATAATTATGTCGAACTAGTTTCA
    TGGTTAGAAAATCCGAATTCGAAAAAAGTCGTATATGATGCTAAAAAAACATATGTAGCA
    TCACATAGATTGGGGATTGATATTCAAAATATTTCTTTCGATATTATGTTGGCCAGTTAT
    ATAATTGATCCATCTCGTACGATTAGTGATGTTCAATCAGTTGTTTCATTGTACGGTCAA
    AGTTTTGTGAAAGACGATGTGAGTATATATGGAAAAGGTAAGAAATTTAAGGTACCTGAA
    GATGATGTTTTAAATCCATATGTTGCTTCTATTACTGATGCAATTTACTTTGCGAAACCA
    AATATGGATAAACAATTAGAAGAATACAATCAGGTAGAACTCTTGGCTGATTTAGAGCTA
    CCGCTAGCTAAGATTTTAAGTGAAATGGAAGAAATTGGTATATTTACAGATGTTCATGAT
    TTAGAAGAAATGGAAAAAGAAATTCAAGAAAAATTAGACGTCTTGATTCGAAATATCCAT
    GATGCAGCTGGTGAAGATTTTAATATAAATTCTCCTAAGCAATTAGGTGTTGTATTGTTT
    GAGACATTACAATTACCTGTTATTAAAAAGACGAAAACAGGATATTCTACTGCTGTAGAT
    GTCTTAGAGCAATTGCAAGGTGAACATCCTATCATTGATTATATTTTAGAATATCGTCAA
    TTATCTAAGTTACAGTCTACTTATGTTGAAGGATTGCAAAAGGTAATTAGTGATGATCAA
    CGTATCCATACACGTTTTAATCAAACTTTAGCGCAAACTGGACGTTTATCAAGTGTAGAT
    CCTAATTTACAAAATATTCCGGTTAGACTTGAAGAAGGGCGTAAAATAAGAAAAGCCTTT
    AAACCAACTTCAAAAGATAGCGTTATATTATCAGCAGATTATTCTCAAATTGAATTGCGT
    GTATTAGCACACATTACACAAGATGAGAGTATGAAAGAAGCATTTATCAACGGCGATGAT
    ATTCATACAGCAACTGCTATGAAAGTATTTGGTGTAGAAGCTGATCAAGTCGATAGTTTA
    ATGCGTCGTCAAGCAAAAGCAGTTAACTTTGGAATTGTTTATGGGATAAGTGATTATGGT
    TTAAGTCAAAGTTTAGGTATTACTCGTAAAAAAGCAAAAGCATTCATTGATGATTATTTA
    GCTAGTTTCCCAGGTGTAAAACAATATATGTCTGATATTGTAAAAGATGCCAAAGCTTTA
    GGTTACGTGGAAACATTGCTACATCGTCGACGCTATATTCCTGATATTACGAGTCGTAAC
    TTTAATTTACGCGGCTTTGCTGAACGTACTGCTATGAATACGCCAATACAGGGCAGTGCT
    GCAGATATCATTAAACTGGCAATGGTTAAATTTGCTCAAAAAATGAAAGAGACAACATAT
    CAAGCTAAACTATTATTACAAGTACACGATGAATTAATTTTTGAAGTACCTAAGTCAGAA
    GTAGATTCATTTAGTGAATTTGTAGAAGAGATAATGGAAAATGCATTGCAATTAGATGTT
    CCATTAAAAGTAGACTCAAGTTATGGTGCAACCTGGTATGATGCAAAATAA
    60
    120
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    2160
    2220
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    2400
    2460
    2520
    2580
    2631

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08880 [old locus tag: SACOL1737 ]
  • symbol: SACOL_RS08880
  • description: DNA polymerase I
  • length: 876
  • theoretical pI: 4.77368
  • theoretical MW: 99194
  • GRAVY: -0.32911

Function[edit source | edit]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymeraseDeoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase I (TIGR00593; EC 2.7.7.7; HMM-score: 1054.3)
    flap structure-specific endonuclease (TIGR03674; EC 3.1.-.-; HMM-score: 19.4)
  • TheSEED:
  • PFAM:
    no clan definedDNA_pol_A; DNA polymerase family A (PF00476; HMM-score: 520.6)
    PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 183.2)
    5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 122)
    RNase_H (CL0219) DNA_pol_A_exo1; 3'-5' exonuclease (PF01612; HMM-score: 23.2)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 16.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.176
    • Ymax_pos: 20
    • Cmax: 0.121
    • Cmax_pos: 30
    • Smax: 0.371
    • Smax_pos: 11
    • Smean: 0.274
    • D: 0.214
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446961052 NCBI
  • RefSeq: WP_001038308 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKILKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRYELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYTPDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHLDEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKKLEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNILKFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNISFDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVASITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEIQEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQGEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQNIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIHTATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYLASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQGSAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENALQLDVPLKVDSSYGATWYDAK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08880 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]