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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09020 [old locus tag: SACOL1764 ]
  • pan locus tag?: SAUPAN004367000
  • symbol: SACOL_RS09020
  • pan gene symbol?: thiI
  • synonym:
  • product: tRNA 4-thiouridine(8) synthase ThiI

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS09020 [old locus tag: SACOL1764 ]
  • symbol: SACOL_RS09020
  • product: tRNA 4-thiouridine(8) synthase ThiI
  • replicon: chromosome
  • strand: -
  • coordinates: 1803263..1804486
  • length: 1224
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1803263..1804486) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAGTATGATCACTTGCTTGTTAGATACGGGGAGTTAACATTAAAGGGTTCAAATAGA
    AAGAAATTTGTAAATCAATTAAGAAATAATGTAAATAAGTCATTAAAAGGACTTGATGGG
    TTTGTCGTTAAAGGCAAACGAGATCGTATGTATATTGAACTTGAAGACCATGCAGATATA
    AATGAAATAACATATCGATTATCAAAAATTTTCGGTATTAAATCTATTAGTCCAGTATTA
    AAAGTAGAAAAAACAATAGAGGCAATAAGTGCAGCGGCAATTAAATTTGCGCAGCAATTT
    GAAGAAAACAGCACATTTAAAATTGATGTGAAGCGTGCCGATAAAAATTTCCCAATGGAT
    ACGTATGAATTACAGCGTGAATTGGGTGGTGCAGTATTGAAGCACTTCGACAATATTTCA
    GTGAATGTCAAACGTCCAGATCATGAAATTCGAGTGGAAGTTAGATTAGATGCAATTTAT
    ATGTATGAAGAAGTTGTTCCGGGTTCAGGTGGATTACCAGTTGGTACTGGTGGTAAGACG
    TTACTAATGCTTTCAGGCGGTATAGACTCACCAGTTGCTGGGATGGAAGTGATGAGACGT
    GGCGTAACAATTGAAGCGATTCATTTCCATAGTCCACCATTTACAAGTGATCAAGCAAAA
    GAAAAAGTTATTGAATTGACACGTATTTTAGCTGAACGTGTTGGACCAATTAAATTGCAT
    ATTGTACCATTTACAGAATTGCAAAAACAGGTAAATAAAGTTGTACATCCAAGATATACA
    ATGACTTCAACGAGACGTATGATGATGCGTGTTGCTGATAAATTAGTACATCAAATAGGG
    GCTTTAGCTATTGTAAATGGTGAAAACCTAGGGCAGGTAGCCAGTCAAACACTTCATAGC
    ATGTATGCAATTAATAATGTAACTTCTACTCCTGTATTACGTCCTTTATTAACTTACGAT
    AAAGAAGAAATTATTATTAAATCGAAAGAAATTGGTACATTTGAAACATCTATTCAACCA
    TTTGAAGATTGTTGTACAATTTTCACCCCTAAAAATCCAGTAACCGAACCAAACTTTGAT
    AAGGTAGTCCAATATGAAAGTGTCTTTGATTTTGAAGAGATGATTAATCGTGCTGTTGAA
    AATATTGAAACACTTGAAATAACTAGTGATTATAAAACTATTAAAGAACAGCAAACAAAC
    CAATTAATAAACGACTTTTTATAA
    60
    120
    180
    240
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    420
    480
    540
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    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1224

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS09020 [old locus tag: SACOL1764 ]
  • symbol: SACOL_RS09020
  • description: tRNA 4-thiouridine(8) synthase ThiI
  • length: 407
  • theoretical pI: 7.36521
  • theoretical MW: 46209
  • GRAVY: -0.279853

Function[edit source | edit]

  • reaction:
    EC 2.8.1.4?  ExPASy
    tRNA sulfurtransferaseL-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP?
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 394)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminetRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 394)
    Unknown functionGeneralqueuosine biosynthesis protein QueC (TIGR00364; HMM-score: 20.9)
    Hypothetical proteinsConservedputative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 20.4)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 13)
  • TheSEED:
  • PFAM:
    HUP (CL0039) ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 204)
    no clan definedTHUMP; THUMP domain (PF02926; HMM-score: 66)
    HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 19.9)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 13.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.129
    • Ymax_pos: 27
    • Cmax: 0.112
    • Cmax_pos: 27
    • Smax: 0.23
    • Smax_pos: 20
    • Smean: 0.144
    • D: 0.135
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446795397 NCBI
  • RefSeq: WP_000872653 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIYMYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTNQLINDFL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS09020 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]