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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09300 [old locus tag: SACOL1814 ]
  • pan locus tag?: SAUPAN004452000
  • symbol: SACOL_RS09300
  • pan gene symbol?:
  • synonym:
  • product: alpha/beta hydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS09300 [old locus tag: SACOL1814 ]
  • symbol: SACOL_RS09300
  • product: alpha/beta hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 1870425..1871252
  • length: 828
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1870425..1871252) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTGGAAGTGGGAAGCTGAAAATGATGCGAAAGGCGTCGTTGTTATTGCTCATAATATA
    TTAGAACATACGGGAAGATATGCATATGTTATTACGATGTTAAGAAGAAATGGCTATCAT
    GTGATTATGGGAGATTTGCCTGGTCAAGGTCAAACTTCAAGAGCTAATAAAGGACAAATT
    GAAAACTTTCAAACGTATCACGAAAGTTTGCTAGACTGGTTAAAGATAGCTAATGAATAT
    AAAATTCCTACTTATGTATTAGGGGTAGGACTTGGCGGTTTGATTTTATTAAATTTACTT
    GAAAAAGTAGAATTGCCAATCGAGGGGATGATGTTAATTTCTCCTATGCTAGAACTTCAA
    AAGAATGGAAAAAATCGTAAAGATAAACTTGTTTCAAATATAGGTAAAATTTCAAAAGAC
    ACACGTTTTAATGTTGGTGTAGAACCAAAAGATTTAACACGTAATTTAGAAATTGTTGAA
    GAAACAGTTAACGATGGTTTAATGTTAAAAAAAGCAACGTATCATTGGTATAATACAATT
    AATGAGACGATGAAAGATACGATGGCTCATATTCATGACATTCAACCAATGCCAACGCTA
    TTAATGTATGGAACGAAGGATTTAATTGTTGATACTAGGGCAATTGATGAGTTTAAAGAA
    AAATATCAAACACCTGAATTATATTTTAAAGCTTGGCAAGGTTTTTACCACGAGGTTCAC
    AATGAGCCAGAACGTGATGAAGTAATGCGTTATATTCTGACTTTCTTAAATAACAGCGTC
    AATACAATGGGCTTTATTGTTGAAGATGACGAAATTGTAGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    828

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS09300 [old locus tag: SACOL1814 ]
  • symbol: SACOL_RS09300
  • description: alpha/beta hydrolase
  • length: 275
  • theoretical pI: 5.40101
  • theoretical MW: 31865.4
  • GRAVY: -0.362182

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 32.7)
    MetabolismEnergy metabolismPhotosynthesisputative magnesium chelatase accessory protein (TIGR03056; HMM-score: 20.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinpimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 20)
  • TheSEED:
  • PFAM:
    AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 144.7)
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 48)
    DUF1057; Alpha/beta hydrolase of unknown function (DUF1057) (PF06342; HMM-score: 21.3)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 20.1)
    DLH; Dienelactone hydrolase family (PF01738; HMM-score: 17.8)
    LIDHydrolase; Lipid-droplet associated hydrolase (PF10230; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.158
    • Ymax_pos: 56
    • Cmax: 0.169
    • Cmax_pos: 56
    • Smax: 0.187
    • Smax_pos: 54
    • Smean: 0.077
    • D: 0.127
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446188241 NCBI
  • RefSeq: WP_000266096 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMGDLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEKVELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVEETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEFKEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS09300 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]