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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09550 [old locus tag: SACOL1862 ]
  • pan locus tag?: SAUPAN004556000
  • symbol: SACOL_RS09550
  • pan gene symbol?: hsdM2
  • synonym:
  • product: type I restriction-modification system subunit M

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS09550 [old locus tag: SACOL1862 ]
  • symbol: SACOL_RS09550
  • product: type I restriction-modification system subunit M
  • replicon: chromosome
  • strand: -
  • coordinates: 1914658..1916397
  • length: 1740
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1914658..1916397) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
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    361
    421
    481
    541
    601
    661
    721
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    841
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    1021
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    1141
    1201
    1261
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    1381
    1441
    1501
    1561
    1621
    1681
    ATGATTTTGAAAGCATTTGAAAGCTACAACATTTCTATAAAATTTTTCAATAACAATTGC
    GCCACTAAAACTCAAAATTTCCACCACCAACATCCAAATTATCAACATCGCAACATAACC
    AAATGTTATAATAAATCTATTACACAAAGAGATAAATTACTTATGCAAAGGCGGAGGAAT
    CACATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGG
    TCGATTGCGAATGATTTAAGAGGGAACATGGATGCGAGTGAATTCCGTAATTACATTTTA
    GGCTTGATTTTCTATCGCTTCTTATCTGAAAAAGCCGAACAAGAATATGCAGATGCCTTG
    TCAGGTGAAGACATCACGTATCAAGAAGCATGGGCAGATGAAGAATATCGTGAAGACTTA
    AAAGCAGAATTAATTGATCAAGTCGGTTACTTCATTGAGCCACAAGATTTATTCAGTGCG
    ATGATTCGTGAAATTGAAACGCAAGATTTCGATATAGAACATCTAGCGACGGCGATTCGC
    AAAGTTGAAACATCTACATTAGGTGAAGAAAGTGAAAATGACTTTATCGGGCTGTTCAGC
    GATATGGATTTGAGTTCAACGCGACTAGGTAACAATGTCAAAGAACGTACTGCTTTAATC
    TCTAAAGTCATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGAT
    ATGTTAGGTGATGCATATGAATTCCTAATCGGGCGCTTTGCGGCGACAGCGGGTAAAAAA
    GCAGGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGAC
    GGTAAAGATAAATTACGTCACGTGTATGACCCAACATGTGGTTCAGGTTCACTGTTGTTA
    CGTGTTGGTAAAGAAACACAAGTGTATCGTTATTTCGGTCAAGAACGTAACAATACTACA
    TACAACTTAGCACGCATGAATATGTTATTACATGATGTGCGTTATGAGAACTTCGATATC
    CGTAATGATGACACATTGGAAAACCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATT
    GCGAACCCACCGTATAGTGCGAAATGGACTGCAGATTCAAAGTTTGAAAATGACGAACGA
    TTCAGTGGTTACGGCAAACTTGCGCCTAAGTCTAAAGCAGACTTTGCCTTTATTCAACAC
    ATGGTACATTACCTAGACGATGAAGGTACCATGGCCGTTGTACTCCCACATGGTGTATTA
    TTCCGAGGTGCTGCAGAAGGTGTCATTCGTCGTTATTTAATTGAAGAAAAGAACTACTTA
    GAAGCTGTGATTGGTTTGCCAGCGAATATTTTCTATGGGACAAGTATTCCAACATGTATT
    TTAGTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAAT
    GATTTTGAAAAAGGAAAAAATCAAAATCATTTAAGCGATGCCCAAGTCGAACGTATTATA
    GACACATATAAGCGTAAGGAAACAATTGATAAATATAGCTACAGCGCGACACTACAAGAG
    ATTGCCGATAACGATTACAACCTAAATATACCGAGATATGTCGATACATTCGAAGAAGAA
    GCACCGATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGATAAAGAAATCGCA
    GAAATTGAGCAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA
    60
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    1140
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    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS09550 [old locus tag: SACOL1862 ]
  • symbol: SACOL_RS09550
  • description: type I restriction-modification system subunit M
  • length: 579
  • theoretical pI: 4.77081
  • theoretical MW: 66947.7
  • GRAVY: -0.562522

Function[edit source | edit]

  • reaction:
    EC 2.1.1.72?  ExPASy
    Site-specific DNA-methyltransferase (adenine-specific)S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
  • TIGRFAM:
    Genetic information processingDNA metabolismRestriction/modificationtype I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 467.2)
    Genetic information processingProtein fateProtein modification and repairprotein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 36.5)
    Genetic information processingDNA metabolismRestriction/modificationtype II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 36.4)
    Genetic information processingProtein fateProtein modification and repairmethyltransferase, HemK family (TIGR00536; HMM-score: 15.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 14.2)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationprotein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 13.8)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 372)
    no clan definedHsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 75)
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 24.2)
    Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 23.7)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 15.7)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.2)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 14.1)
    N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 13.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.207
    • Ymax_pos: 24
    • Cmax: 0.129
    • Cmax_pos: 24
    • Smax: 0.414
    • Smax_pos: 20
    • Smean: 0.302
    • D: 0.244
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_011443644 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS09550 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]