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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS10280 [old locus tag: SACOL1966 ]
  • pan locus tag?: SAUPAN004934000
  • symbol: SACOL_RS10280
  • pan gene symbol?: pcrA
  • synonym:
  • product: DNA helicase PcrA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS10280 [old locus tag: SACOL1966 ]
  • symbol: SACOL_RS10280
  • product: DNA helicase PcrA
  • replicon: chromosome
  • strand: -
  • coordinates: 2028699..2030891
  • length: 2193
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2028699..2030891) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAATGCGTTATTAAATCATATGAATACAGAGCAAAGTGAAGCTGTAAAGACAACAGAA
    GGACCATTGTTAATTATGGCAGGTGCTGGTTCAGGGAAGACACGTGTTTTAACACATAGA
    ATTGCTTATTTATTAGACGAAAAAGATGTCTCACCATACAATGTTTTGGCTATTACTTTT
    ACAAATAAAGCTGCAAGAGAAATGAAAGAACGTGTTCAAAAATTAGTAGGTGATCAAGCA
    GAAGTTATTTGGATGTCAACATTCCACTCAATGTGTGTTCGTATTTTACGTCGTGATGCA
    GATCGAATTGGTATAGAACGCAATTTTACGATAATTGATCCTACAGACCAAAAATCTGTT
    ATTAAAGACGTCTTAAAAAATGAAAATATTGATAGTAAAAAGTTTGAACCTCGTATGTTT
    ATCGGTGCGATCAGTAATTTGAAAAATGAACTTAAAACACCTGCAGATGCTCAAAAAGAA
    GCCACAGATTATCACTCGCAAATGGTAGCAACGGTTTATAGTGGATATCAACGCCAATTG
    TCACGTAATGAAGCGTTAGATTTTGATGACCTTATTATGACAACGATTAACTTATTTGAG
    CGTGTACCAGAAGTTCTAGAATATTATCAGAACAAATTCCAATATATTCATGTAGATGAG
    TATCAAGATACTAATAAAGCACAATACACATTAGTTAAATTATTAGCAAGTAAGTTTAAA
    AACTTATGTGTTGTAGGTGACTCAGATCAGTCAATTTATGGTTGGCGTGGTGCTGATATT
    CAAAATATCTTATCATTTGAAAAAGACTATCCAGAAGCGAATACAATCTTTTTAGAGCAA
    AATTATCGTTCGACGAAAACGATTTTAAATGCGGCTAACGAAGTGATTAAAAATAATTCT
    GAACGTAAGCCAAAAGGACTGTGGACTGCAAATACGAATGGTGAGAAAATTCATTACTAT
    GAAGCAATGACGGAACGTGATGAAGCGGAATTTGTAATACGAGAAATTATGAAGCATCAA
    CGTAATGGTAAGAAATATCAAGATATGGCAATTTTATATAGAACGAATGCACAATCACGT
    GTACTTGAGGAAACATTCATGAAATCTAATATGCCATACACAATGGTTGGTGGCCAAAAG
    TTCTATGACCGTAAAGAAATCAAAGATTTATTAAGTTATTTGCGTATAATTGCCAATAGT
    AATGATGACATTAGTTTGCAACGTATTATTAATGTTCCAAAAAGAGGTGTAGGTCCTTCA
    TCTGTTGAAAAAGTTCAAAACTATGCACTTCAAAACAATATCAGTATGTTTGATGCACTT
    GGAGAAGCTGATTTTATCGGATTGTCAAAAAAGGTGACACAAGAGTGTCTTAATTTTTAC
    GAGTTAATCCAAAGCTTGATAAAAGAACAAGAATTTTTAGAAATTCATGAAATAGTTGAT
    GAAGTATTACAAAAGTCTGGCTATCGAGAAATGCTTGAACGTGAAAATACATTAGAATCT
    CGAAGTAGATTAGAAAACATCGATGAATTTATGTCAGTACCAAAAGACTATGAGGAAAAT
    ACCCCATTAGAAGAACAGTCATTAATTAACTTTTTAACGGATTTATCGTTAGTAGCTGAT
    ATTGATGAGGCAGATACTGAAAATGGCGTAACACTAATGACGATGCACTCGGCTAAGGGC
    CTTGAATTTCCAATTGTCTTTATAATGGGGATGGAAGAATCTTTATTCCCACATATTAGA
    GCGATTAAGAGTGAAGATGATCATGAAATGCAAGAAGAACGTCGTATTTGTTATGTAGCA
    ATTACAAGGGCTGAAGAGGTGTTATATATCACTCATGCGACATCAAGAATGTTATTTGGT
    CGCCCTCAGTCAAATATGCCATCCAGATTTTTAAAGGAAATTCCAGAATCACTATTAGAA
    AATCATTCAAGTGGCAAACGACAAACGATACAACCTAAGGCAAAACCTTTTGCTAAACGC
    GGATTTAGTCAACGAACAACGTCAACGAAAAAACAAGTATTGTCATCTGATTGGAATGTA
    GGTGACAAAGTGATGCATAAAGCCTGGGGAGAAGGCATGGTGAGTAATGTAAACGAGAAA
    AATGGCTCAATCGAACTAGATATTATCTTTAAATCACAAGGGCCAAAACGTTTGTTAGCG
    CAATTTGCACCAATTGAAAAAAAGGAGGATTAA
    60
    120
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    480
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    720
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    1560
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    1920
    1980
    2040
    2100
    2160
    2193

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS10280 [old locus tag: SACOL1966 ]
  • symbol: SACOL_RS10280
  • description: DNA helicase PcrA
  • length: 730
  • theoretical pI: 5.63681
  • theoretical MW: 84073
  • GRAVY: -0.546438

Function[edit source | edit]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicaseATP + H2O = ADP + phosphate
    EC 3.6.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 1189.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA helicase II (TIGR01075; EC 3.6.4.12; HMM-score: 737.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 661.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairhelicase-exonuclease AddAB, AddA subunit (TIGR02785; EC 3.1.-.-,3.6.4.12; HMM-score: 206.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairdouble-strand break repair helicase AddA (TIGR02784; HMM-score: 158.8)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexodeoxyribonuclease V, beta subunit (TIGR00609; EC 3.1.11.5; HMM-score: 156.8)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 292.8)
    UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 267.2)
    AAA_19; AAA domain (PF13245; HMM-score: 122.7)
    Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 37.9)
    UvrD_C_2; UvrD-like helicase C-terminal domain (PF13538; HMM-score: 37.5)
    AAA_30; AAA domain (PF13604; HMM-score: 33.2)
    Exonuc_V_gamma; Exodeoxyribonuclease V, gamma subunit (PF04257; HMM-score: 20.2)
    AAA_11; AAA domain (PF13086; HMM-score: 18.1)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.3)
    Vps51 (CL0295) Vps51; Vps51/Vps67 (PF08700; HMM-score: 14.4)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL_RS03055DNA-directed RNA polymerase subunit beta'  [1] (data from MRSA252)
    SACOL_RS05910excinuclease ABC subunit C  [1] (data from MRSA252)
    SACOL_RS11650DNA-directed RNA polymerase subunit alpha  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.19
    • Ymax_pos: 16
    • Cmax: 0.148
    • Cmax_pos: 16
    • Smax: 0.306
    • Smax_pos: 7
    • Smean: 0.233
    • D: 0.207
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446915665 NCBI
  • RefSeq: WP_000992921 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKEATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIREIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIVFIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQSNMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGDKVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS10280 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2

Relevant publications[edit source | edit]