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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02123
  • pan locus tag?: SAUPAN004934000
  • symbol: SAOUHSC_02123
  • pan gene symbol?: pcrA
  • synonym:
  • product: ATP-dependent DNA helicase PcrA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02123
  • symbol: SAOUHSC_02123
  • product: ATP-dependent DNA helicase PcrA
  • replicon: chromosome
  • strand: -
  • coordinates: 1996719..1998911
  • length: 2193
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAATGCGTTATTAAATCATATGAATACAGAGCAAAGTGAAGCTGTAAAGACAACAGAA
    GGACCATTGTTAATTATGGCAGGTGCTGGTTCAGGGAAGACACGTGTTTTAACACATAGA
    ATTGCTTATTTATTAGACGAAAAAGATGTCTCACCATACAATGTTTTGGCTATTACTTTT
    ACAAATAAAGCTGCAAGAGAAATGAAAGAACGTGTTCAAAAATTAGTAGGTGATCAAGCA
    GAAGTTATTTGGATGTCAACATTCCACTCAATGTGTGTTCGTATTTTACGTCGTGATGCA
    GATCGAATTGGTATAGAACGCAATTTTACGATAATTGATCCTACAGACCAAAAATCTGTT
    ATTAAAGACGTCTTAAAAAATGAAAATATTGATAGTAAAAAGTTTGAACCTCGTATGTTT
    ATCGGTGCGATCAGTAATTTGAAAAATGAACTTAAAACACCTGCAGATGCTCAAAAAGAA
    GCCACAGATTATCACTCGCAAATGGTAGCAACGGTTTATAGTGGATATCAACGCCAATTG
    TCACGTAATGAAGCGTTAGATTTTGATGACCTTATTATGACAACGATTAACTTATTTGAG
    CGTGTACCAGAAGTTCTAGAATATTATCAGAACAAATTCCAATATATTCATGTAGATGAG
    TATCAAGATACTAATAAAGCACAATACACATTAGTTAAATTATTAGCAAGTAAGTTTAAA
    AACTTATGTGTTGTAGGTGACTCAGATCAGTCAATTTATGGTTGGCGTGGTGCTGATATT
    CAAAATATCTTATCATTTGAAAAAGACTATCCAGAAGCGAATACAATCTTTTTAGAGCAA
    AATTATCGTTCGACGAAAACGATTTTAAATGCGGCTAACGAAGTGATTAAAAATAATTCT
    GAACGTAAGCCAAAAGGACTGTGGACTGCAAATACGAATGGTGAGAAAATTCATTACTAT
    GAAGCAATGACGGAACGTGATGAAGCGGAATTTGTAATACGAGAAATTATGAAGCATCAA
    CGTAATGGTAAGAAATATCAAGATATGGCAATTTTATATAGAACGAATGCACAATCACGT
    GTACTTGAGGAAACATTCATGAAATCTAATATGCCATACACAATGGTTGGTGGCCAAAAG
    TTCTATGACCGTAAAGAAATCAAAGATTTATTAAGTTATTTGCGTATAATTGCCAATAGT
    AATGATGACATTAGTTTGCAACGTATTATTAATGTTCCAAAAAGAGGTGTAGGTCCTTCA
    TCTGTTGAAAAAGTTCAAAACTATGCACTTCAAAACAATATCAGTATGTTTGATGCACTT
    GGAGAAGCTGATTTTATCGGATTGTCAAAAAAGGTGACACAAGAGTGTCTTAATTTTTAC
    GAGTTAATCCAAAGCTTGATAAAAGAACAAGAATTTTTAGAAATTCATGAAATAGTTGAT
    GAAGTATTACAAAAGTCTGGCTATCGAGAAATGCTTGAACGTGAAAATACATTAGAATCT
    CGAAGTAGATTAGAAAACATCGATGAATTTATGTCAGTACCAAAAGACTATGAGGAAAAT
    ACCCCATTAGAAGAACAGTCATTAATTAACTTTTTAACGGATTTATCGTTAGTAGCTGAT
    ATTGATGAGGCAGATACTGAAAATGGCGTAACACTAATGACGATGCACTCGGCTAAGGGC
    CTTGAATTTCCAATTGTCTTTATAATGGGGATGGAAGAATCTTTATTCCCACATATTAGA
    GCGATTAAGAGTGAAGATGATCATGAAATGCAAGAAGAACGTCGTATTTGTTATGTAGCA
    ATTACAAGGGCTGAAGAGGTGTTATATATCACTCATGCGACATCAAGAATGTTATTTGGT
    CGCCCTCAGTCAAATATGCCATCCAGATTTTTAAAGGAAATTCCAGAATCACTATTAGAA
    AATCATTCAAGTGGCAAACGACAAACGATACAACCTAAGGCAAAACCTTTTGCTAAACGC
    GGATTTAGTCAACGAACAACGTCAACGAAAAAACAAGTATTGTCATCTGATTGGAATGTA
    GGTGACAAAGTGATGCATAAAGCCTGGGGAGAAGGCATGGTGAGTAATGTAAACGAGAAA
    AATGGCTCAATCGAACTAGATATTATCTTTAAATCACAAGGGCCAAAACGTTTGTTAGCG
    CAATTTGCACCAATTGAAAAAAAGGAGGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2193

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02123
  • symbol: SAOUHSC_02123
  • description: ATP-dependent DNA helicase PcrA
  • length: 730
  • theoretical pI: 5.63681
  • theoretical MW: 84073
  • GRAVY: -0.546438

Function[edit source | edit]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicaseATP + H2O = ADP + phosphate
    EC 3.6.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 1189.2)
    and 5 more
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA helicase II (TIGR01075; EC 3.6.4.12; HMM-score: 737.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 661.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairhelicase-exonuclease AddAB, AddA subunit (TIGR02785; EC 3.1.-.-,3.6.4.12; HMM-score: 206.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairdouble-strand break repair helicase AddA (TIGR02784; HMM-score: 158.8)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexodeoxyribonuclease V, beta subunit (TIGR00609; EC 3.1.11.5; HMM-score: 156.8)
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, bacterial UvrD and related helicases ATP-dependent DNA helicase UvrD/PcrA 
    and 1 more
    CBSS-393121.3.peg.1913 ATP-dependent DNA helicase UvrD/PcrA 
  • PFAM:
    P-loop_NTPase (CL0023) UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 292.8)
    UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 267.2)
    and 9 more
    AAA_19; AAA domain (PF13245; HMM-score: 122.7)
    Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 37.9)
    UvrD_C_2; UvrD-like helicase C-terminal domain (PF13538; HMM-score: 37.5)
    AAA_30; AAA domain (PF13604; HMM-score: 33.2)
    Exonuc_V_gamma; Exodeoxyribonuclease V, gamma subunit (PF04257; HMM-score: 20.2)
    AAA_11; AAA domain (PF13086; HMM-score: 18.1)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.3)
    Vps51 (CL0295) Vps51; Vps51/Vps67 (PF08700; HMM-score: 14.4)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_01102(uvrC)excinuclease ABC subunit C  [2] (data from MRSA252)
    SAOUHSC_00525DNA-directed RNA polymerase subunit beta'  [2] (data from MRSA252)
    SAOUHSC_02485DNA-directed RNA polymerase subunit alpha  [2] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.19
    • Ymax_pos: 16
    • Cmax: 0.148
    • Cmax_pos: 16
    • Smax: 0.306
    • Smax_pos: 7
    • Smean: 0.233
    • D: 0.207
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKEATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIREIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIVFIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQSNMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGDKVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. 2.0 2.1 2.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  5. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

S Iordanescu
Characterization of the Staphylococcus aureus chromosomal gene pcrA, identified by mutations affecting plasmid pT181 replication.
Mol. Gen. Genet.: 1993, 241(1-2);185-92
[PubMed:8232203] [WorldCat.org] (P p)