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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS10295 [old locus tag: SACOL1969 ]
  • pan locus tag?: SAUPAN004937000
  • symbol: SACOL_RS10295
  • pan gene symbol?: purB
  • synonym:
  • product: adenylosuccinate lyase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS10295 [old locus tag: SACOL1969 ]
  • symbol: SACOL_RS10295
  • product: adenylosuccinate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 2032163..2033458
  • length: 1296
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2032163..2033458) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATTGAACGCTATTCTAGAGAAGAAATGTCTAATATTTGGACTGATCAAAATCGCTAT
    GAAGCATGGTTAGAAGTGGAAATTTTAGCATGTGAAGCATGGAGTGAATTAGGACATATA
    CCGAAAGCTGACGTACAAAAAATTCGTCAAAACGCAAAGGTAAATGTCGAACGTGCACAA
    GAAATTGAACAAGAAACGCGCCATGATGTTGTAGCCTTTACTAGACAAGTTTCTGAAACG
    CTAGGTGAAGAACGTAAGTGGGTACATTATGGTTTAACTTCTACTGATGTTGTAGATACA
    GCTTTAAGTTTCGTTATTAAACAAGCAAATGACATTATTGAAAAAGATTTAGAAAGATTT
    ATCGACGTTTTAGCTGAAAAAGCAAAAAATTATAAATATACATTGATGATGGGACGTACA
    CATGGAGTGCATGCAGAACCAACTACATTTGGTGTCAAAATGGCATTGTGGTACACGGAA
    ATGCAACGTAACTTACAACGTTTCAAACAAGTAAGAGAAGAAATTGAAGTAGGGAAAATG
    AGTGGTGCAGTAGGTACTTTTGCTAACATTCCTCCTGAAATAGAAAGTTATGTGTGTAAA
    CATTTAGGTATTGGCACAGCGCCTGTATCAACGCAAACATTGCAACGCGATCGACATGCA
    TACTATATTGCGACATTAGCATTAATCGCGACATCACTAGAAAAATTTGCCGTTGAAATA
    CGTAACCTTCAAAAAACCGAAACGAGAGAAGTTGAAGAAGCATTTGCAAAAGGCCAAAAA
    GGTTCATCTGCAATGCCTCATAAACGAAATCCAATTGGTTCTGAAAATATCACAGGTATT
    TCAAGAGTCATTCGTGGTTATATTACTACAGCTTATGAGAATGTGCCATTATGGCATGAA
    AGAGATATTTCTCATTCTTCTGCTGAGCGTATTATGTTACCAGATGTTACAATAGCACTA
    GATTATGCATTGAATCGTTTCACAAATATTGTGGACCGTTTAACTGTATTTGAAGATAAT
    ATGCGTAATAACATAGATAAAACATTTGGTCTGATTTTCTCACAACGTGTCTTATTAGCA
    TTAATTAATAAAGGTATGGTTCGTGAAGAAGCATACGACAAAGTACAACCAAAAGCGATG
    ATATCTTGGGAAACGAAAACACCGTTCCGTGAATTAATCGAACAAGATGAGTCTATTACA
    AGTGTTTTAACAAAAGAAGAATTGGATGAATGTTTCGATCCTAAACATCATTTGAATCAA
    GTTGACACTATTTTCGAACGCGCTGGATTAGCATAA
    60
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    1296

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS10295 [old locus tag: SACOL1969 ]
  • symbol: SACOL_RS10295
  • description: adenylosuccinate lyase
  • length: 431
  • theoretical pI: 5.6693
  • theoretical MW: 49603
  • GRAVY: -0.435963

Function[edit source | edit]

  • reaction:
    EC 4.3.2.2?  ExPASy
    Adenylosuccinate lyaseN6-(1,2-dicarboxyethyl)AMP = fumarate + AMP(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisadenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 581.4)
    MetabolismEnergy metabolismOther3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 196)
    MetabolismAmino acid biosynthesisGlutamate familyargininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 85.6)
    MetabolismEnergy metabolismAmino acids and aminesaspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 62.6)
    MetabolismEnergy metabolismTCA cyclefumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 56.7)
  • TheSEED:
  • PFAM:
    no clan definedLyase_1; Lyase (PF00206; HMM-score: 253.4)
    ADSL_C; Adenylosuccinate lyase C-terminus (PF10397; HMM-score: 92.5)
    ASL_C; Adenylosuccinate lyase C-terminal (PF08328; HMM-score: 15.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 44
    • Cmax: 0.128
    • Cmax_pos: 44
    • Smax: 0.117
    • Smax_pos: 42
    • Smean: 0.058
    • D: 0.083
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446495024 NCBI
  • RefSeq: WP_000572878 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTEMQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS10295 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]