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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS10350 [old locus tag: SACOL1980 ]
  • pan locus tag?: SAUPAN004952000
  • symbol: SACOL_RS10350
  • pan gene symbol?:
  • synonym:
  • product: pectate lyase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS10350 [old locus tag: SACOL1980 ]
  • symbol: SACOL_RS10350
  • product: pectate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 2043475..2044572
  • length: 1098
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2043475..2044572) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTATATCAATATGAAAGATTATGGGTTAACAGGCATAAACAAAACTAAAGATACTCGA
    GCAATACAACGTGCGTTAAATCGTGGAAGATGTAAACCAACGACAGTTTATATACCGAAA
    GGGACGTATGATATTTGCAAACCATTAACGATATATGGCAATACAACACTTTTGTTAGAT
    AATGAAACTATTTTACGCCGATGTCATTCTGGTCCTTTATTAAAAAATGGTCGTCGCTTT
    GGTTTTTATCGTGGTTATAATGGACACAGTCATATTCATATTAAAGGCGGCAAGTTTGAT
    ATGAATGGTGTATCGTATCCTTATAACAATACAGCTATGTGCATTGGGCATGCTGAAGAT
    ATTCAATTAATAGGTGTGACCATTAAGAATGTAGTGAGTGGTCATGCAATTGATGCTTGT
    GGGATTAACGGACTCTATATTAAAAGCTGTTCATTTGAAGGATTCATAGACTATAGTGGC
    GAACGCTTTTATTCTGAAGCAATACAATTAGACATTCAAGTACCTGGTGCTTTTCCAAAA
    TTCGGAACGACAGATGGTACGATAACGAAAAATGTCATTATCGAAGATTGTTATTTTGGA
    CCTTCAGAATTGCCCGAAATGGGAAGTTGGAATCGTGCTATTGGCTCACATGCAAGTAGA
    CATAATCGATACTATGAGAATATTCATATTAGAAATAATATATTTGAAGATATACAAGGT
    TATGCATTAACTCCCTTGAAGTATAAAGATGCTTTCATTATTAATAATAAGTTTATTAAC
    TGTGAGGGTGGCATTAGATATTTAGGAGTTAGAGATGGTAAAAATGCAGCAGATGTGATG
    ACAGGAAAAGACTTAGGTTCCCAAGCAGGCATAAATATGAATATAATTGGAAATGAATTT
    AAAGGATCAATGTCTAAAGATGCGATACATGTACGTAATTATAATAATGTTAAACATAAA
    GATGTATTAATCGTTGGGAATACATTCAATAATTCGACTCAATCAATTCATTTAGAAGAT
    ATTGATACAGTGTTTTTAAGTCCTGTTGAAGCGGGTATTCAAGTTACTACAATCAATGTA
    GATGAAATAAAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1098

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS10350 [old locus tag: SACOL1980 ]
  • symbol: SACOL_RS10350
  • description: pectate lyase
  • length: 365
  • theoretical pI: 8.51329
  • theoretical MW: 40719
  • GRAVY: -0.338904

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    parallel beta-helix repeat (TIGR03804; HMM-score: 4.7)
  • TheSEED:
  • PFAM:
    Pec_lyase-like (CL0268) Pectate_lyase_3; Pectate lyase superfamily protein (PF12708; HMM-score: 46.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 22
    • Cmax: 0.106
    • Cmax_pos: 22
    • Smax: 0.166
    • Smax_pos: 19
    • Smean: 0.133
    • D: 0.124
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446197875 NCBI
  • RefSeq: WP_000275730 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYDICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMNGVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSGERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHASRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNAADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQSIHLEDIDTVFLSPVEAGIQVTTINVDEIKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS10350 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]