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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS11070 [old locus tag: SACOL2116 ]
  • pan locus tag?: SAUPAN005416000
  • symbol: SACOL_RS11070
  • pan gene symbol?: murA2
  • synonym:
  • product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS11070 [old locus tag: SACOL2116 ]
  • symbol: SACOL_RS11070
  • product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2175902..2177161
  • length: 1260
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2175902..2177161) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCTCAAGAGGTAATAAAAATAAGAGGTGGACGCACACTAAATGGGGAAGTTAATATC
    AGTGGCGCGAAAAACAGTGCTGTAGCTATCATTCCTGCAACATTATTAGCTCAAGGACAT
    GTGAAATTAGAAGGGTTACCGCAAATCTCTGATGTTAAAACTTTAGTGAGTTTATTAGAA
    GATTTAAATATTAAGGCATCACTAAATGGTACGGAATTAGAAGTTGATACAACTGAAATA
    CAAAATGCTGCATTACCAAATAATAAAGTTGAATCATTACGTGCTTCATACTATATGATG
    GGTGCCATGTTAGGTAGATTTAAAAAATGTGTGATTGGTTTACCGGGAGGATGTCCACTT
    GGGCCACGTCCAATTGATCAACATATTAAAGGATTTAAAGCTTTAGGTGCTGAAATTGAT
    GAATCAAGTACGACATCAATGAAAATCGAAGCTAAAGAGTTAAAAGGTGCACATATTTTT
    CTAGATATGGTTAGCGTAGGTGCAACAATTAACATTATGCTAGCTGCAGTTTATGCAACA
    GGACAAACTGTAATTGAAAATGCTGCTAAAGAACCGGAAGTAGTTGATGTTGCTAACTTC
    TTAACAAGTATGGGAGCTAATATTAAGGGAGCGGGTACATCAACAATTAAAATCAATGGC
    GTCAAAGAATTACATGGTTCTGAGTATCAAGTTATACCTGATAGAATTGAAGCAGGCACA
    TATATGTGTATCGCTGCAGCATGTGGAGAAAATGTTATATTAAATAATATTGTTCCGAAA
    CATGTAGAAACATTAACTGCTAAATTTAGTGAATTAGGTGTGAATGTTGATGTAAGAGAC
    GAAAGAATTCGCATCAATAATAATGCGCCATATCAATTTGTTGATATTAAAACACTAGTA
    TATCCAGGTTTTGCTACTGATTTGCAACAGCCTATTACACCATTATTATTTATGGCAAAT
    GGTCCTTCATTTGTAACTGATACGATTTATCCGGAGCGTTTTAAACATGTTGAAGAATTA
    AAGCGCATGGGTGCAAATATTGAAGTTGACGAAGGCACAGCAACGATAAAACCATCAACA
    TTACATGGTGCTGAAGTTTATGCTAGCGATTTAAGAGCAGGAGCTTGTTTAATTATTGCT
    GGTTTAATTGCTGAGGGTGTAACTACAATTTACAATGTTAAACATATTTATAGAGGTTAT
    ACAGATATTGTAGAACACTTAAAAGCTTTAGGTGCAGATATTTGGACGGAAACTGTATAG
    60
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    840
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    960
    1020
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    1140
    1200
    1260

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS11070 [old locus tag: SACOL2116 ]
  • symbol: SACOL_RS11070
  • description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  • length: 419
  • theoretical pI: 5.46141
  • theoretical MW: 45074.7
  • GRAVY: 0.072315

Function[edit source | edit]

  • reaction:
    EC 2.5.1.7?  ExPASy
    UDP-N-acetylglucosamine 1-carboxyvinyltransferasePhosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylglucosamine 1-carboxyvinyltransferase (TIGR01072; EC 2.5.1.7; HMM-score: 526.9)
    MetabolismAmino acid biosynthesisAromatic amino acid family3-phosphoshikimate 1-carboxyvinyltransferase (TIGR01356; EC 2.5.1.19; HMM-score: 87.8)
  • TheSEED:
  • PFAM:
    EPT_RTPC (CL0290) EPSP_synthase; EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275; HMM-score: 300.5)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.173
    • Ymax_pos: 30
    • Cmax: 0.161
    • Cmax_pos: 30
    • Smax: 0.364
    • Smax_pos: 36
    • Smean: 0.138
    • D: 0.16
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445968747 NCBI
  • RefSeq: WP_000046602 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLEGLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGAMLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIFLDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKINGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNVDVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERFKHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYNVKHIYRGYTDIVEHLKALGADIWTETV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS11070 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]