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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS12825 [old locus tag: SACOL2443 ]
  • pan locus tag?: SAUPAN005990000
  • symbol: SACOL_RS12825
  • pan gene symbol?: kimA
  • synonym:
  • product: amino acid permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS12825 [old locus tag: SACOL2443 ]
  • symbol: SACOL_RS12825
  • product: amino acid permease
  • replicon: chromosome
  • strand: -
  • coordinates: 2502380..2504209
  • length: 1830
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2502380..2504209) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTTCAATCAATTTAAAAGACTTATTATAGGGCAACCTAAAAAAAACAGAGAACTAAAA
    GACGAAAAGATATCGAAGTTTAAAGGGTTAGCGATTCTTTCGTCGGATGCATTATCTTCA
    GTGGCTTACGGTCCCGAACAAATACTGATTACACTATCAGTAGTGGGTGCAGTTGCAACT
    TGGTATACTTTACCGATTGCAGGTGCTGTTCTGATCTTATTAGCTGCTTTAATTATGTCT
    TATAGGCAAATTATTTATGCATATCCTAAAGGTGGCGGCGCGTATATGGTGTCCAAGACC
    AATTTAGGAGAAAAATGGGGATTGCTTGCAGGAGGCTCATTATTAGTCGATTACATACTG
    ACTGTCGCTGTTAGTATATCATCTGGTGCTGATGCATTTGTTGCTGCATTCCCAAGTTTA
    TATGGGCATAAAGTACTGATTGCATGTTTACTTGTACTTTTTATTTTAATATTAAATTTA
    CGTGGTTTAACTGAATCTGCCACCGTATTATCCTATCCAGTATATCTTTTCATTATAGGG
    TTAGTGATATTAATATTTATAGGTACTTTCCGTGTGGCGACAGGAGATATTCAACCACAT
    ATGCATGCATCAGTAGGAACTGCGGTTCCTGGAGTAACATTATTCTTGCTATTAAAAGCA
    TTTTCATCAGGGGCTTCATCGTTAACGGGTGTTGAAGCTATTTCAAATGCGGTAACCAAT
    TTTAGAGAGCCAAGTGCTAAAAATGCTGTGAAAACGTTAATTGCTATGGGTTCAATTTTA
    GCGTTTTTATTAGTTGGTATCGTTGGTTTAGCATATGTTTATGGAATTTTGCCACAAACG
    GAAACGACAGTTTTATCACAATTAGCTATGCAAATATTTGGTGATAATGCTGCCTTCTAT
    TTTGTACAGGCAACGACCGTGATGATTTTGGTATTAGCAGCCAACACTGGATTTACAGCT
    TTTCCGATGTTAGCGGCTTCAATGTCTAAAGATAAATATATGCCTAGAATGTTTACTGTT
    AGAGGTGATCGCTTAGGCTACTCTAACTCGATTATTATTTTAGGTGTATTAGCCATTATT
    TTAATTATTGTATTTGATGGTATGACTGAAGATTTAATTCCATTGTATGCGGTTGGTGTA
    TTTATTCCATTTACACTAGCACAATTCGGTATGGTTATTAAATGGATTCATGAACGTCCT
    AAGAATTGGTTAAGTAAGTTGTCTGTGAACTTACTTGGTGGTATCGTGACATTTATTGTT
    TTTATGATTTTACTCATAACTAAATTTAGCCAAGTATGGCCGATACTTATTTTCTTGCCG
    TTCGTGGTGATTTTCTTCTTGAAAATTAACAAACATTATCGAGATATCGCAGAACAATTA
    CGTTCTGACATTGATGTTCTTAATGTGGACGTGGTAGATCGTAATTTAGCAATTGTGCCG
    ATAACAAGTATTACAACAGCAGTTGATAAGTCTATTTATTACGCACAAATGCTTGCGAAT
    AATGATGTCATAGCAGTACATGTATCATTTGGAGATGAAGATGAAAAAGCATTCCAAGAG
    AAATGGAAACGTCATTTCCCAGATGTAAGATTAGTTATTTTACATTCGGAATATCGCAGT
    ATTATACGACCAATTTCACGTTTTATCGATAAGATTAATCGTAAAGCCAACGATCAAAAC
    TATATGATTACAGTAGTGATACCAGAATTTATTACTAAGAAACGTTGGCATAATTTATTA
    CACAATCAAACTAGTTTGCGTATGAAATTGTATTTAATTTATCAAAAGAATGTTAACGTA
    TGTACAATTCCATTTAAACTTAAAAAATAG
    60
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    1560
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    1830

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS12825 [old locus tag: SACOL2443 ]
  • symbol: SACOL_RS12825
  • description: amino acid permease
  • length: 609
  • theoretical pI: 10.0029
  • theoretical MW: 67572.8
  • GRAVY: 0.602791

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid transporter (TIGR00909; HMM-score: 30.1)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesethanolamine permease (TIGR00908; HMM-score: 16)
  • TheSEED:
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 116.7)
    no clan definedNUFIP1; Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1) (PF10453; HMM-score: 15.3)
    DUF2244; Integral membrane protein (DUF2244) (PF10003; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 20
    • Cmax: 0.12
    • Cmax_pos: 70
    • Smax: 0.244
    • Smax_pos: 9
    • Smean: 0.179
    • D: 0.156
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

  • GI: 446405194 NCBI
  • RefSeq: WP_000483049 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYGPEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNLGEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNLRGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLLKAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGILPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYMPRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMVIKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKINKHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHVSFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVVIPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS12825 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]