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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13130 [old locus tag: SACOL2505 ]
  • pan locus tag?: SAUPAN006107000
  • symbol: SACOL_RS13130
  • pan gene symbol?: sasG
  • synonym:
  • product: accumulation-associated protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS13130 [old locus tag: SACOL2505 ]
  • symbol: SACOL_RS13130
  • product: accumulation-associated protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2559220..2562951
  • length: 3732
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2559220..2562951) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    ATGAGAGATAAGAAAGGACCGGTAAATAAAAGAGTAGATTTTCTATCAAATAAATTGAAT
    AAATATTCAATAAGAAAATTTACAGTTGGAACAGCATCTATTTTAATTGGCTCACTAATG
    TATTTGGGAACTCAACAAGAGGCAGAAGCAGCTGAAAACAATATTGAGAATCCAACTACA
    TTAAAAGATAATGTCCAATCAAAAGAAGTGAAGATTGAAGAAGTAACAAACAAAGACACT
    GCACCACAGGGTGTAGAAGCTAAATCTGAAGTAACTTCAAACAAAGACACAATCGAACAT
    GAACCATCAGTAAAAGCTGAAGATATATCAAAAAAGGAGGATACACCAAAAGAAGTAGCT
    GATGTTGCTGAAGTTCAGCCGAAATCGTCAGTCACTCATAACGCAGAGACACCTAAGGTT
    AGAAAAGCTCGTTCTGTTGATGAAGGCTCTTTTGATATTACAAGAGATTCTAAAAATGTA
    GTTGAATCTACCCCAATTACAATTCAAGGTAAAGAACATTTTGAAGGTTACGGAAGTGTT
    GATATACAAAAAAAACCAACAGATTTAGGGGTATCAGAGGTAACCAGGTTTAATGTTGGT
    AATGAAAGTAATGGTTTGATAGGAGCTTTACAATTAAAAAATAAAATAGATTTTAGTAAG
    GATTTCAATTTTAAAGTTAGAGTGGCAAATAACCATCAATCAAATACCACAGGTGCTGAT
    GGTTGGGGGTTCTTATTTAGTAAAGGAAATGCAGAAGAATATTTAACTAATGGTGGAATC
    CTTGGGGATAAAGGTCTGGTAAATTCAGGCGGATTTAAAATTGATACTGGATACATTTAT
    ACAAGTTCCATGGACAAAACTGAAAAGCAAGCTGGACAAGGTTATAGAGGATACGGAGCT
    TTTGTGAAAAATGACAGTTCTGGTAATTCACAAATGGTTGGAGAAAATATTGATAAATCA
    AAAACTAATTTTTTAAACTATGCGGACAATTCAACTAATACATCAGATGGAAAGTTTCAT
    GGGCAACGTTTAAATGATGTCATCTTAACTTATGTTGCTTCAACTGGTAAAATGAGAGCA
    GAATATGCTGGTAAAACTTGGGAGACTTCAATAACAGATTTAGGTTTATCTAAAAATCAG
    GCATATAATTTCTTAATTACATCTAGTCAAAGATGGGGCCTTAATCAAGGGATAAATGCA
    AATGGCTGGATGAGAACTGACTTGAAAGGTTCAGAGTTTACTTTTACACCAGAAGCGCCA
    AAAACAATAACAGAATTAGAAAAAAAAGTTGAAGAGATTCCATTCAAGAAAGAACGTAAA
    TTTAATCCGGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAG
    AAGACAATAACGACACCAACACTAAAAAATCCATTAACTGGAGTAATTATTAGTAAAGGT
    GAACCAAAAGAAGAGATTACAAAAGATCCGATTAATGAATTAACAGAATACGGACCTGAA
    ACAATAGCGCCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAG
    GAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGACGTAGTTAGACCACCG
    GTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAG
    ATTCCATTCGAGAAAGAACGTAAATTTAATCCGGATTTAGCACCAGGGACAGAAAAAGTA
    ACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACACCAACACTAAAAAATCCATTA
    ACTGGAGTAATTATTAGTAAAGGTGAACCAAAAGAAGAAATCACAAAAGATCCGATTAAT
    GAATTAACAGAATACGGACCAGAAACGATAACACCAGGTCATCGAGACGAATTTGATCCG
    AAGTTACCAACAGGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAA
    ACAGGAGACGTAGTTAGACCACCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGA
    GACTCGATTGTAGAAAAAGAAGAAATTCCATTCAAGAAAGAACGTAAATTTAATCCGGAT
    TTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACG
    ACGCCAACACTAAAAAATCCATTAACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAA
    GAAATCACAAAAGATCCGATTAATGAATTAACAGAATACGGACCAGAAACGATAACACCA
    GGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAGGAAGTTCCAGGT
    AAACCAGGAATTAAGAATCCAGAAACAGGAGACGTAGTTAGACCACCGGTCGATAGCGTA
    ACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAAATTCCATTCAAG
    AAAGAACGTAAATTTAATCCTGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGA
    CAAAAAGGTGAGAAGACAATAACGACGCCAACACTAAAAAATCCATTAACTGGAGAAATT
    ATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAAGATCCGATTAATGAATTAACAGAA
    TACGGACCAGAAACGATAACACCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACA
    GGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGATGTA
    GTTAGACCACCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTA
    GAAAAAGAAGAAATTCCATTCGAGAAAGAACGTAAATTTAATCCTGATTTAGCACCAGGG
    ACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACGCCAACACTA
    AAAAATCCATTAACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAA
    GATCCAGTTAATGAATTAACAGAATTCGGTGGCGAGAAAATACCGCAAGGTCATAAAGAT
    ATCTTTGATCCAAACTTACCAACAGATCAAACGGAAAAAGTACCAGGTAAACCAGGAATC
    AAGAATCCAGACACAGGAAAAGTGATCGAAGAGCCAGTGGATGATGTGATTAAACACGGA
    CCAAAAACGGGTACACCAGAAACAAAAACAGTAGAGATACCGTTTGAAACAAAACGTGAG
    TTTAATCCAAAATTACAACCTGGTGAAGAGCGAGTGAAACAAGAAGGACAACCAGGAAGT
    AAGACAATCACAACACCAATCACAGTGAACCCATTAACAGGTGAAAAAGTTGGCGAGGGT
    CAACCAACAGAAGAGATCACAAAACAACCAGTAGATAAGATTGTAGAGTTCGGTGGAGAG
    AAACCAAAAGATCCAAAAGGACCTGAAAACCCAGAGAAGCCGAGCAGACCAACTCATCCA
    AGTGGCCCAGTAAATCCTAACAATCCAGGATTATCGAAAGACAGAGCAAAACCAAATGGC
    CCAGTTCATTCAATGGATAAAAATGATAAAGTTAAAAAATCTAAAATTGCTAAAGAATCA
    GTAGCTAATCAAGAGAAAAAACGAGCAGAATTACCAAAAACAGGTTTAGAAAGCACGCAA
    AAAGGTTTGATCTTTAGTAGTATAATTGGAATTGCTGGATTAATGTTATTGGCTCGTAGA
    AGAAAGAATTAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS13130 [old locus tag: SACOL2505 ]
  • symbol: SACOL_RS13130
  • description: accumulation-associated protein
  • length: 1243
  • theoretical pI: 5.49702
  • theoretical MW: 136344
  • GRAVY: -0.956718

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 46.4)
    calcium-translocating P-type ATPase, PMCA-type (TIGR01517; EC 3.6.3.8; HMM-score: 39.2)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 23.9)
    zinc metalloproteinase 18-residue repeat (TIGR04227; HMM-score: 10)
  • TheSEED:
  • PFAM:
    G5 (CL0593) SasG_E; E domain (PF17041; HMM-score: 461.6)
    G5; G5 domain (PF07501; HMM-score: 241.3)
    no clan definedYSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 44.4)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 25.2)
    DMP12; DNA-mimic protein (PF16779; HMM-score: 22.5)
    Ribosomal_L28; Ribosomal L28 family (PF00830; HMM-score: 22.1)
    2C_adapt; 2-cysteine adaptor domain (PF08793; HMM-score: 18.5)
    NTF2 (CL0051) DUF3828; Protein of unknown function (DUF3828) (PF12883; HMM-score: 16.3)
    HAD (CL0137) Cation_ATPase; Cation transport ATPase (P-type) (PF13246; HMM-score: 8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide length 51 aa
    • Ymax: 0.626
    • Ymax_pos: 51
    • Cmax: 0.66
    • Cmax_pos: 51
    • Smax: 0.722
    • Smax_pos: 47
    • Smean: 0.345
    • D: 0.517
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447127837 NCBI
  • RefSeq: WP_001205093 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGTQQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEPSVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNVVESTPITIQGKEHFEGYGSVDIQKKPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDFSKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDTGYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNTSDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRWGLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKVEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGVIISKGEPKEEITKDPINELTEYGPETIAPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGVIISKGEPKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLIFSSIIGIAGLMLLARRRKN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS13130 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]