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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13345 [old locus tag: SACOL2549 ]
  • pan locus tag?: SAUPAN006189000
  • symbol: SACOL_RS13345
  • pan gene symbol?:
  • synonym:
  • product: alpha/beta hydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS13345 [old locus tag: SACOL2549 ]
  • symbol: SACOL_RS13345
  • product: alpha/beta hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2606694..2607662
  • length: 969
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2606694..2607662) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCGTAAAAAATGGTCTACACTTGCGTTTGGATTTTTAGTTGCAGCATACGCACATATT
    AGAATTAAAGAAAAACGCAGTGTGAAAAGTTATATGTTAGAACAAGGTATACGATTATCT
    AGAGCTAAGCGTCGTTTTATGTATAAAGAAGAAGCGATGAAAGCATTAGAAAAAATGGCG
    CCACAGACAGCAGGCGAATATGAGGGAACCAATTATCAGTTTAAGATGCCAGTAAAAGTG
    GATAAGCACTTCGGTTCAACCGTTTATACCGTTAACGATAAACAAGATAAGCATCAACGC
    GTTGTATTATATGCACATGGAGGCGCATGGTTCCAAGACCCACTCAAAATTCATTTCGAA
    TTTATTGATGAACTTGCAGAAACACTCAATGCTAAAGTCATCATGCCAGTATATCCGAAG
    ATTCCGCATCAAGATTATCAAGCGACGTATGTGCTTTTTGAAAAGTTGTACCATGATTTA
    TTGAATCAAGTAGCAGATTCTAAACAAATCGTTGTAATGGGTGACTCTGCGGGCGGTCAA
    ATTGCTTTATCATTTGCTCAATTGTTAAAAGAAAAACATATTGTGCAACCAGGACATATT
    GTATTAATTTCACCAGTTTTAGATGCAACGATGCAGCATCCTGAAATTCCTGACTACTTA
    AAGAAAGACCCAATGGTAGGTGTGGATGGCAGTGTGTTCTTAGCTGAACAATGGGCAGGG
    GACACACCTTTAGATAACTACAAAGTATCACCAATTAATGGTGACTTAGATGGTTTAGGT
    CGTATTACATTAACTGTTGGTACAAAAGAAGTACTGTACCCAGATGCTTTGAACTTATCG
    CAATTGTTGAGTGCGAAAGGTATCGAACATGACTTTATACCTGGATATTACCAATTCCAT
    ATTTATCCAGTATTTCCGATACCAGAGCGACGACGCTTTTTATACCAAGTGAAAAATATT
    ATTAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS13345 [old locus tag: SACOL2549 ]
  • symbol: SACOL_RS13345
  • description: alpha/beta hydrolase
  • length: 322
  • theoretical pI: 9.11945
  • theoretical MW: 36847.4
  • GRAVY: -0.26087

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityprobable F420-dependent oxidoreductase, Rv1855c family (TIGR03560; HMM-score: 11.9)
  • TheSEED:
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 104.8)
    Say1_Mug180; Steryl acetyl hydrolase (PF10340; HMM-score: 55.6)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 19.7)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 18.6)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 17.1)
    Esterase; Putative esterase (PF00756; HMM-score: 15.2)
    AXE1; Acetyl xylan esterase (AXE1) (PF05448; HMM-score: 14.1)
    no clan definedUPF0240; Uncharacterised protein family (UPF0240) (PF06784; HMM-score: 13.4)
    AB_hydrolase (CL0028) BAAT_C; BAAT / Acyl-CoA thioester hydrolase C terminal (PF08840; HMM-score: 13.1)
    Lipase_3; Lipase (class 3) (PF01764; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.177
    • Ymax_pos: 11
    • Cmax: 0.11
    • Cmax_pos: 67
    • Smax: 0.502
    • Smax_pos: 1
    • Smean: 0.272
    • D: 0.214
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447150906 NCBI
  • RefSeq: WP_001228162 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKRRFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVVLYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDLLNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPDYLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDALNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS13345 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]