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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13815 [old locus tag: SACOL2639 ]
  • pan locus tag?: SAUPAN006326000
  • symbol: SACOL_RS13815
  • pan gene symbol?: cysJ
  • synonym:
  • product: assimilatory sulfite reductase (NADPH) flavoprotein subunit

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS13815 [old locus tag: SACOL2639 ]
  • symbol: SACOL_RS13815
  • product: assimilatory sulfite reductase (NADPH) flavoprotein subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 2698709..2700589
  • length: 1881
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2698709..2700589) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
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    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    TTGAAATTAAATACATCTAATAGTCCATTTACAGAAAAACAAGTCACAGAGATTAACAAT
    CTGCTGCAAACATTAACAGAGAGCCAACAACAGTGGCTTAGTGGTTTTTTACTAGCTAAT
    AGTAACGATACGACAAGTGATAGTAATCAACAACAATTAGAGACAGAAGTGTGGCAACAA
    TCACAAATATCAGAAGAACAAGCAACTTCAACAACGTATATGTTACAAAATAAAGAGCCA
    CATATCGAAGCTAATCAGCGGCATGTTACAGTGCTATATGGTTCTGAATCAGGTAATGCC
    ATGCGTTTAGCTGAAATTTTTTCAGAACGTTTAAGTGATATCGGACATCAAGTTGTTTTG
    ATGTCAATGGATGAATATGATACGACAAACATCGCGCAGTTAGAAGATTTATTTATTATT
    ACGTCTACTCATGGTGAAGGAGAACCGCCTGATAATGCATGGGATTTCTTTGAATTTTTA
    GAAGACGATAACGCACCTAATTTAAATCATGTGAGATATTCAGTACTAGCTTTAGGTGAT
    CAAACATATGAATTTTTCTGTCAAGCCGGTAAAGATGTAGATGTTTTACTAGAAAATCTA
    GGCGCTGAGCGTATATGTAAGCGTGTAGATTGTGATATTGATTATGAAGAAGACGCAGAA
    AAGTGGATGGCAGACATCATTAATATTATTGATACCACATCAGAAGGTATTCAAAGTGAA
    TCGGTGATAAGTGAATCAATTAAGTCTGCCAAAGAAAAGAAATATTCTAAATCAAATCCA
    TACCAAGCAGAAGTATTAGCGAATATCAATTTAAATGGTACCGATTCAAATAAAGAAACA
    CGACATATAGAATTTTTACTTGATGATTTTAGTGAATCATATGAACCAGGAGATTGTATA
    GTAGCATTACCGCAAAACGACCCTGAATTGGTTGAAAAACTAATATTCATGTTAGGTTGG
    GATCCGCAATCTCCGGTGCCAATTAATGATCATGGTGATACAGTTCCTATTGTTGAAGCA
    CTAACATCACATTTTGAATTTACTAAATTAACATTGCCATTATTGAAAAATGCAGATATC
    TATTTTGACAATGAAGAATTATCTGAACGTATTCAAGATGAGTCATGGGCGCGTGAATAT
    GTTATAAATCGGGACTTTATAGATTTAATAACAGATTTTCCAACTATAGAATTACAACCT
    GAGAATATGTATCAAATCCTTAGAAAATTACCACCAAGAGAGTATTCGATTTCTAGTAGT
    TTTATGGCAACGCCAGATGAAGTGCATATTACCGTTGGTACGGTTCGTTATCAAGCACAT
    GGACGTGAGAGAAAAGGTGTATGCTCGGTTCATTTTGCTGAGCGAATTAAACCAGGCGAT
    ATAGTACCAATTTATTTGAAGAAAAATCCGAACTTCAAATTTCCGATGAAGCAAGATATA
    CCGGTTATTATGATTGGACCAGGTACTGGAATTGCTCCTTTTAGAGCATATTTACAAGAA
    CGTGAAGAACTTGGTATGACTGGAAAAACATGGTTGTTCTTTGGTGATCAACACCGTAGT
    TCTGACTTTTTATATGAAGAAGAAATAGAAGAATGGCTTGAAAATGGAAACTTAACACGC
    GTAGATTTAGCATTTTCAAGAGACCAAGAACACAAAGAATATGTACAGCATCGTATAATG
    GAAGAAAGTAAACGTTTCAATGAATGGATTGAGCAAGGCGCAGCAATCTATATTTGTGGC
    GATGAAAAATGTATGGCGAAAGATGTCCATCAAGCCATTAAAGATGTATTGGTAAAAGAA
    CGTCATATTTCTCAAGAAGAAGCAGAGTTATTATTGCGACAAATGAAACAACAACAACGC
    TATCAACGTGATGTTTATTAG
    60
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    1560
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    1860
    1881

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS13815 [old locus tag: SACOL2639 ]
  • symbol: SACOL_RS13815
  • description: assimilatory sulfite reductase (NADPH) flavoprotein subunit
  • length: 626
  • theoretical pI: 4.2983
  • theoretical MW: 72272.2
  • GRAVY: -0.579073

Function[edit source | edit]

  • reaction:
    EC 1.8.1.2?  ExPASy
    Assimilatory sulfite reductase (NADPH)H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH
  • TIGRFAM:
    sulfite reductase [NADPH] flavoprotein, alpha-component (TIGR01931; EC 1.8.1.2; HMM-score: 712.8)
    MetabolismEnergy metabolismOtherbenzoyl-CoA oxygenase/reductase, BoxA protein (TIGR03224; EC 1.14.12.21; HMM-score: 63.9)
    MetabolismEnergy metabolismElectron transportflavodoxin (TIGR01753; HMM-score: 57.6)
    MetabolismEnergy metabolismOtherphenylacetate-CoA oxygenase/reductase, PaaK subunit (TIGR02160; HMM-score: 33.1)
    MetabolismEnergy metabolismElectron transportflavodoxin (TIGR01752; HMM-score: 26.5)
    MetabolismCentral intermediary metabolismSulfur metabolismsulfite reductase, subunit B (TIGR02911; EC 1.8.-.-; HMM-score: 17.3)
    putative ribonucleotide reductase-associated flavodoxin (TIGR01754; HMM-score: 16.2)
  • TheSEED:
  • PFAM:
    Flavoprotein (CL0042) Flavodoxin_1; Flavodoxin (PF00258; HMM-score: 114.7)
    FAD_Lum_binding (CL0076) FAD_binding_1; FAD binding domain (PF00667; HMM-score: 93.8)
    NAD_Ferredoxin (CL0091) NAD_binding_1; Oxidoreductase NAD-binding domain (PF00175; HMM-score: 69)
    Flavoprotein (CL0042) Flavodoxin_5; Flavodoxin domain (PF12724; HMM-score: 13)
    no clan definedANTAR; ANTAR domain (PF03861; HMM-score: 12.1)
    Anti-adapt_IraP; Sigma-S stabilisation anti-adaptor protein (PF10796; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FAD, FMN
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.181
    • Ymax_pos: 11
    • Cmax: 0.121
    • Cmax_pos: 49
    • Smax: 0.413
    • Smax_pos: 5
    • Smean: 0.334
    • D: 0.24
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446697806 NCBI
  • RefSeq: WP_000775152 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMRLAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFLEDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS13815 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]