From AureoWiki
Jump to: navigation, search
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00042
  • pan locus tag?: SAUPAN000385000
  • symbol: SAOUHSC_00042
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00042
  • symbol: SAOUHSC_00042
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 44596..45816
  • length: 1221
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACTGATTTGTTGAAAATTAAAAACTTCAGATTGTTCTTTTTAGCAGAAATAATTTCA
    GCATTCGGAGTTGGAATTAGTACAGTTGGGGCTAATTGGTATTTAATCGACAAAACTAAC
    GACTCCCAACTATTAGGTATTATGTTAGCTTTAAATGTATTATCCGGTTTCTTAGCTTCT
    CCTATAATAGGTGGACTAGCCGATAAATATAATAGACGAAATATTATTTTAATCACCTAT
    CTTCTTCAGGTAATATTATATTTACTCATTGTCATAGCACTCGTAATGATAGGATTTGAG
    ACCTATTTAGTGATTGGATTTGCGATTGTTAATGGGATAGGTTGGACTACGTATATGGCA
    ACATCTAGAAGTTTAGTTAAACAGATATTAAAACCAGATCAATATACAGATGCAAATTCT
    CTATTAGAGATTAGTTTGCAAACAGGTATGTTTATTGCAGGTGGATTATCAGGAATATTA
    TATAAAATAAATGGATTCACTCTAATTATAGCGATGACTATAATGATGTTTCTAATCAGC
    ATTTTTATGTTATTTAGATTGCATGTAGATAAACCAACTCATTCAGAGGAAGAATCAACA
    AATAGCTTATTGCAAGAATATTTATTAGGATGGAAATTTTTAAAAGACAACATGATGATA
    TTTATTTTTGGAGTTATTTCAATCATACCAATGGTGTTTACAATGATCTTTAACATATCA
    TTACCAGGATATGTTTATAATGTTTTAAAACTTTCGTCTGTACAATTTGGTTTTTCAGAT
    ATGTTATATGGCATTGGAGGATTATGTGCAGGTTTAATTTCAGCTATTCTTTCGAAGAAA
    ATCTCAACTAAAGTATTGATATTTTTGTTATATTTCATATTAGTCATAAATTCAGCACTA
    TTTATTTGGATAAACTCAGCATTTTACTTATTCATAGGATCATTTATACTAGGCTACTCA
    ATTTCATCAATCAGAATTTATATGAATACAGCTATAATGAACACTGTTTCAGATAAATAT
    GTCGGTCGCTCATTTACGATATGGACGTCAATTTCATTGTTACTGCAAAGTTTAATTGCT
    CCATTTTTAGGAAGATGGATTAATGAAATTAATGATAAATTCGGTTTCTATATTATACTC
    ATTTTATCCTTACTCATATTTGTTACACTGCTGCTTGTTAACAAAACAGACAAAATAAAA
    TATGCACATAAAGAAGAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1221

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00042
  • symbol: SAOUHSC_00042
  • description: hypothetical protein
  • length: 406
  • theoretical pI: 9.43757
  • theoretical MW: 45716.5
  • GRAVY: 0.86798

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsH+ Antiporter protein (TIGR00900; HMM-score: 165.1)
    multidrug resistance protein (TIGR00880; HMM-score: 67.8)
    MetabolismTransport and binding proteinsUnknown substrateMFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 26.6)
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 19.4)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 19.4)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscation transport protein (TIGR00898; HMM-score: 16.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 12.6)
    MetabolismTransport and binding proteinsAnionsnitrite transporter (TIGR00886; HMM-score: 8.8)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidssugar (Glycoside-Pentoside-Hexuronide) transporter (TIGR00792; HMM-score: 7.9)
  • TheSEED:  
    FIG01108443: hypothetical protein 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 109)
    MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 60)
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 30.5)
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 18.7)
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 9.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.21
    • Ymax_pos: 32
    • Cmax: 0.187
    • Cmax_pos: 32
    • Smax: 0.355
    • Smax_pos: 13
    • Smean: 0.241
    • D: 0.222
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQLLGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETYLVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGILYKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNMMIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAILSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMNTVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLLVNKTDKIKYAHKEE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]