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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00064
  • pan locus tag?: SAUPAN000904000
  • symbol: SAOUHSC_00064
  • pan gene symbol?: norG
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00064
  • symbol: SAOUHSC_00064
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 69186..70583
  • length: 1398
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTAAATATAAAGATATTGCTAGTGACATAAGAGATAAAATAATCACAGGGGATTGG
    TTTTATGGAATGAAAATACCTTCACATAGGCAGTTGGCGATACAGTACAACGTAAATAGA
    GTAACGATTATTAAAAGTATTGAGTTATTAGAAGCTGAAGGATTTATCTATACTAAAGTA
    GGTAGTGGAACATATGTTAATGACTATTTGAATGAAGCACATATTACAAATAAGTGGTCT
    GAAATGATGTTATGGTCCTCTCAACAAAGAAGTCAGTATACGGTGCAATTAATTAATAAA
    ATTGAGACAGATGATTCGTATATACATATAAGTAAAGGTGAATTGGGTATATCGTTAATG
    CCACATATTCAATTGAAAAAAGCCATGTCTAATACAGCCAGTCATATTGAAGACTTATCT
    TTTGGTTATAATAATGGCTATGGTTATATCAAGTTAAGAGATATTATCGTTGAACGAATG
    TCAAAGCAAGGTATAAATGTAGGTAGAGAAAATGTAATGATCACTTCAGGCGCTTTACAT
    GCCATTCAACTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACA
    CCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTT
    CCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAAT
    AAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAG
    CAAAAGAAAAATATAATTACTTATAGCGAAAGACATAATATTCCTATCATTGAAGATGAT
    ATCTTTAGAGATATTTTCTTTAGCGATCCAACTCCTTCTATCAAAACTTATGATAAATTG
    GGAAAAGTTATACATATAAGCAGTTTTTCAAAAACGATTGCACCAGCAATAAGAATAGGT
    TGGATTGTTGCTTCTGAAAAAATAATAGAGCAATTGGCAGATGTAAGAATGCAAATTGAC
    TATGGATCCAGTATTTTGTCACAAATGGTTGTATATGAGATGTTGAAAAATAAGTCTTAT
    GATAAACACTTAGTAAAGTTAAGGTATGTTTTAAAAGATAAACGAGACTTTATGTTAAAC
    ATCCTCAATAATTTATTTAAGGATATAGCACATTGGGAGGTTCCAAGTGGAGGTTATTTT
    GTATGGTTAGTCTTTAAAATAGATATAGATATTAAATATTTATTTTACGAATTGTTAAGT
    AAAGAAAAAATATTAATCAATCCGGGTTACATTTATGGCAGTAAAGAAAAGAGTATAAGG
    CTATCTTTTGCCTTTGAATCAAATGAAAATATTAAGCATGCGCTCTATAAAATTTATACA
    TATGTGAAAAAGGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1398

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00064
  • symbol: SAOUHSC_00064
  • description: hypothetical protein
  • length: 465
  • theoretical pI: 9.31384
  • theoretical MW: 53991.9
  • GRAVY: -0.211398

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    enduracididine biosynthesis enzyme MppQ (TIGR04461; EC 2.-.-.-; HMM-score: 138.1)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 86.3)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 85.2)
    Cellular processesCellular processesBiosynthesis of natural productscapreomycidine synthase (TIGR03947; HMM-score: 82.3)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 77.5)
    MetabolismAmino acid biosynthesisHistidine familyhistidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 67.9)
    MetabolismEnergy metabolismAmino acids and aminestyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 63.6)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 57.2)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 52.2)
    succinyldiaminopimelate transaminase (TIGR03538; EC 2.6.1.17; HMM-score: 47.1)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 46.7)
    MetabolismEnergy metabolismAmino acids and amineshistidine utilization repressor (TIGR02018; HMM-score: 45.8)
    Signal transductionRegulatory functionsDNA interactionshistidine utilization repressor (TIGR02018; HMM-score: 45.8)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 45)
    MetabolismTransport and binding proteinsAnionsphosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 44.7)
    Signal transductionRegulatory functionsDNA interactionsphosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 44.7)
    Signal transductionRegulatory functionsDNA interactionstrehalose operon repressor (TIGR02404; HMM-score: 43.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminthreonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 42.4)
    Signal transductionRegulatory functionsDNA interactionsphosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 36.8)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisfatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 16.8)
    MetabolismFatty acid and phospholipid metabolismDegradationfatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 16.8)
    Signal transductionRegulatory functionsDNA interactionsfatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 16.8)
    MetabolismAmino acid biosynthesisAspartate familyO-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 13.8)
  • TheSEED:  
    Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) 
    Proline, 4-hydroxyproline uptake and utilization Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) 
    Threonine and Homoserine Biosynthesis Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 120)
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 52)
    PLP_aminotran (CL0061) Aminotran_MocR; Alanine-glyoxylate amino-transferase (PF12897; HMM-score: 22.8)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 16.6)
    no clan definedDUF2953; Protein of unknown function (DUF2953) (PF11167; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.089
    • Ymax_pos: 55
    • Cmax: 0.11
    • Cmax_pos: 33
    • Smax: 0.129
    • Smax_pos: 1
    • Smean: 0.062
    • D: 0.079
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTIIKSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIETDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERMSKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHIDVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPIIEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLADVRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHWEVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNENIKHALYKIYTYVKKV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Que Chi Truong-Bolduc, David C Hooper
The transcriptional regulators NorG and MgrA modulate resistance to both quinolones and beta-lactams in Staphylococcus aureus.
J. Bacteriol.: 2007, 189(8);2996-3005
[PubMed:17277059] [WorldCat.org] [DOI] (P p)