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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00070
  • pan locus tag?: SAUPAN000910000
  • symbol: SAOUHSC_00070
  • pan gene symbol?: sarS
  • synonym:
  • product: accessory regulator-like protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00070
  • symbol: SAOUHSC_00070
  • product: accessory regulator-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 75400..76152
  • length: 753
  • essential: no [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAATATAATAACCATGACAAAATTAGAGATTTTATAATCATTGAAGCATATATGTTT
    CGTTTTAAGAAAAAAGTCAAGCCTGAAGTCGATATGACTATAAAAGAATTTATATTACTG
    ACTTATTTATTTCATCAGCAAGAAAACACACTTCCATTTAAGAAGATTGTTTCAGATTTA
    TGTTATAAACAATCGGATTTAGTACAGCATATAAAAGTACTTGTGAAACATTCATATATT
    AGTAAAGTTCGAAGTAAAATTGATGAGCGTAATACTTACATTTCAATATCTGAAGAACAA
    CGAGAGAAAATTGCAGAACGTGTTACATTGTTTGATCAAATCATTAAACAATTTAACCTT
    GCAGATCAAAGTGAATCACAGATGATACCAAAAGATAGTAAAGAATTTCTAAACTTGATG
    ATGTATACAATGTATTTCAAGAATATTATCAAAAAACATCTAACATTAAGTTTTGTAGAA
    TTCACAATTCTAGCTATTATCACTTCTCAAAATAAAAACATCGTTCTTCTTAAAGATTTA
    ATTGAAACAATCCACCATAAATACCCTCAAACTGTTAGAGCTCTCAATAATTTAAAAAAG
    CAAGGCTATCTAATAAAAGAACGCTCAACTGAAGATGAAAGAAAAATTTTAATTCATATG
    GATGACGCGCAGCAAGACCATGCTGAACAATTATTAGCTCAAGTGAATCAATTATTAGCA
    GATAAAGATCATTTACATCTTGTTTTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    753

Protein[edit source | edit]

Protein Data Bank: 1P4X

General[edit source | edit]

  • locus tag: SAOUHSC_00070
  • symbol: SAOUHSC_00070
  • description: accessory regulator-like protein
  • length: 250
  • theoretical pI: 9.31072
  • theoretical MW: 29889.6
  • GRAVY: -0.3956

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionsstaphylococcal accessory regulator family (TIGR01889; HMM-score: 214.6)
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 33.3)
    mobile rSAM pair MarR family regulator (TIGR04472; HMM-score: 14.8)
    Signal transductionRegulatory functionsDNA interactionsheavy metal response regulator (TIGR01387; HMM-score: 14.3)
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsputative phage terminase, small subunit, P27 family (TIGR01558; HMM-score: 13.7)
  • TheSEED:  
    staphylococcal accessory regulator A homologue 
  • PFAM:
    HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 32.7)
    MarR_2; MarR family (PF12802; HMM-score: 30.4)
    Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 25.7)
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 22.1)
    HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 19.2)
    Rrf2; Transcriptional regulator (PF02082; HMM-score: 14.6)
    no clan definedLectin_N; Hepatic lectin, N-terminal domain (PF03954; HMM-score: 13.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.103
    • Ymax_pos: 29
    • Cmax: 0.132
    • Cmax_pos: 29
    • Smax: 0.114
    • Smax_pos: 69
    • Smean: 0.075
    • D: 0.092
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLFHQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQREKIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVEFTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

K Tegmark, A Karlsson, S Arvidson
Identification and characterization of SarH1, a new global regulator of virulence gene expression in Staphylococcus aureus.
Mol. Microbiol.: 2000, 37(2);398-409
[PubMed:10931334] [WorldCat.org] (P p)
A L Cheung, K Schmidt, B Bateman, A C Manna
SarS, a SarA homolog repressible by agr, is an activator of protein A synthesis in Staphylococcus aureus.
Infect. Immun.: 2001, 69(4);2448-55
[PubMed:11254606] [WorldCat.org] [DOI] (P p)
B Saïd-Salim, P M Dunman, F M McAleese, D Macapagal, E Murphy, P J McNamara, S Arvidson, T J Foster, S J Projan, B N Kreiswirth
Global regulation of Staphylococcus aureus genes by Rot.
J. Bacteriol.: 2003, 185(2);610-9
[PubMed:12511508] [WorldCat.org] (P p)
Ronggui Li, Adhar C Manna, Shaodong Dai, Ambrose L Cheung, Gongyi Zhang
Crystal structure of the SarS protein from Staphylococcus aureus.
J. Bacteriol.: 2003, 185(14);4219-25
[PubMed:12837797] [WorldCat.org] (P p)
Katherine A Schmidt, Adhar C Manna, Ambrose L Cheung
SarT influences sarS expression in Staphylococcus aureus.
Infect. Immun.: 2003, 71(9);5139-48
[PubMed:12933857] [WorldCat.org] (P p)
Nadine McCallum, Markus Bischoff, Hideki Maki, Akihito Wada, Brigitte Berger-Bächi
TcaR, a putative MarR-like regulator of sarS expression.
J. Bacteriol.: 2004, 186(10);2966-72
[PubMed:15126456] [WorldCat.org] (P p)
Jinxin Gao, George C Stewart
Regulatory elements of the Staphylococcus aureus protein A (Spa) promoter.
J. Bacteriol.: 2004, 186(12);3738-48
[PubMed:15175287] [WorldCat.org] [DOI] (P p)
Susham Ingavale, Willem van Wamel, Thanh T Luong, Chia Y Lee, Ambrose L Cheung
Rat/MgrA, a regulator of autolysis, is a regulator of virulence genes in Staphylococcus aureus.
Infect. Immun.: 2005, 73(3);1423-31
[PubMed:15731040] [WorldCat.org] [DOI] (P p)
Jan Oscarsson, Caroline Harlos, Staffan Arvidson
Regulatory role of proteins binding to the spa (protein A) and sarS (staphylococcal accessory regulator) promoter regions in Staphylococcus aureus NTCC 8325-4.
Int. J. Med. Microbiol.: 2005, 295(4);253-66
[PubMed:16128400] [WorldCat.org] [DOI] (P p)