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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00282
  • pan locus tag?: SAUPAN001224000
  • symbol: SAOUHSC_00282
  • pan gene symbol?: brnQ2
  • synonym:
  • product: branched-chain amino acid transport system II carrier protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00282
  • symbol: SAOUHSC_00282
  • product: branched-chain amino acid transport system II carrier protein
  • replicon: chromosome
  • strand: -
  • coordinates: 297408..298685
  • length: 1278
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTTATTTTCACTATTTTTTGGAGCCGGAAATTTAATATTCCCGCCCATGCTTGGCCAT
    ACAGCGGGTCAAAATATGTGGATTGGTATGCTAGGCTTTGCCCTTACAGGCATATTACTC
    CCCTTTATTACTGTTATTGTTGTTGCATTTTATGATGAAGGTGTTGAAAGTGTAGGCAAT
    CGTATACATCCATGGTTCGGGTTTATTTTTGCTGTCGTGATTTACATGTCTATCGGAGCA
    TTTTACGGTATTCCACGTGCTGCAAATGTCGCGTACGAAATTGGTACAAGACACATTTTA
    CCTGTGCATAACCAATGGACTTTAATTATATTCGCAGCAATCTTTTTTGCCATCGTTTAC
    TGGATTAGTTTAAATCCATCGAAAATCGTTGATAATTTAGGTAAATTATTAACACCGTTA
    TTACTATTAATGGTCGCTCTATTAAGTATTGCTGTCATTTTCAACCCTGAATCTGCACTA
    AGTGCACCTAAGGATAAATATATAACACATCCTTTCATTTCAGGAAGTTTGGAAGGCTAT
    TTTACAATGGATCTTGTTGCTGCGTTAGCTTTTTCCGTAGTCATTGTCAATGGCTATAAG
    TTTAAAGGCCTCACAGATCGCATGAAAATTTTAAAATATGTCTGCTTTTCAGGTCTTATT
    GCAGCCATATTACTTGGAATGATTTACTTTGCACTTGCATACGTTGGGGCATCAACAGCT
    CCAGGAAACTTTAAAGATGGTACAGATATATTGACGTACAACTCATTACGATTATTTGGT
    TCGTTCGGTAACCTCGTATTTGGAATGACGGTTATCCTTGCATGCCTAACAACATGTATA
    GGACTCGTCAATGCTTGCGCCACATTTACTAAGAAACACGTACCTAAGTTTTCTTATAAA
    ATATTCGCACTTATTTTCTCTATCATAGGGTTCTTATTTACAACACTTGGTTTAGAAATG
    ATTTTAAAAATTGCTGTCCCATTATTGACTTTAATATATCCCGTGTCGATTGCACTTGTA
    CTCATATCATTTGCTAACATGTTTAGCACATTCAGATTCAGTTGGGCCTATCGACTCGCA
    ACTGTTATTACATTGATTATTTCAATTTTACAAATACTAAATAGTTTCAACTTATTACAC
    GGTGTTATTTTGAAATCGTTTATGATGTTACCTTTAGCAGATATCGATTTAGCTTGGCTT
    GTACCATTCATGCTCTTTGCTATTATCGGTTTCATAATCGATGTATTTATACGCCGTCCG
    AAACAAGCGACAACTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1278

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00282
  • symbol: SAOUHSC_00282
  • description: branched-chain amino acid transport system II carrier protein
  • length: 425
  • theoretical pI: 9.58089
  • theoretical MW: 46934.2
  • GRAVY: 1.05247

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesbranched-chain amino acid transport system II carrier protein (TIGR00796; HMM-score: 402.7)
  • TheSEED:  
    Branched-chain amino acid transport system carrier protein 
  • PFAM:
    APC (CL0062) Branch_AA_trans; Branched-chain amino acid transport protein (PF05525; HMM-score: 436.2)
    no clan definedPlant_vir_prot; Plant viral movement protein (PF01307; HMM-score: 18.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.161
    • Ymax_pos: 20
    • Cmax: 0.216
    • Cmax_pos: 52
    • Smax: 0.34
    • Smax_pos: 9
    • Smean: 0.22
    • D: 0.184
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSIAVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGVILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]