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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00392
  • pan locus tag?: SAUPAN002113000
  • symbol: SAOUHSC_00392
  • pan gene symbol?: ssl7
  • synonym:
  • product: superantigen-like protein 7

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00392
  • symbol: SAOUHSC_00392
  • product: superantigen-like protein 7
  • replicon: chromosome
  • strand: +
  • coordinates: 395237..395932
  • length: 696
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    GTGAAATTAAAAACGTTAGCTAAAGCAACATTGGCATTAGGCTTATTAACTACTGGTGTG
    ATTACATCAGAAGGCCAAGCAGTTCAAGCAAAAGAAAAGCAAGAGAGAGTACAACATTTA
    TATGATATTAAAGACTTACATCGATACTACTCATCAGAAAGTTTTGAATTCAGTAATATT
    AGTGGTAAGGTTGAAAATTATAACGGTTCTAACGTTGTACGCTTTAACCAAGAAAATCAA
    AATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGC
    AAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGT
    GGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAA
    GTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGTT
    TCACTGAAAGAACTTGATTTCAAAATTAGACAACATTTAGTTAAAAATTATGGTTTATAT
    AAAGGTACGACTAAATACGGTAAGATCACTATCAATTTGAAAGATGGAGAAAAGCAAGAA
    ATTGATTTAGGTGATAAATTGCAATTCGAGCGCATGGGTGATGTGTTGAATAGTAAGGAT
    ATTAATAAGATTGAAGTGACTTTGAAACAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    696

⊟Protein[edit | edit source]

Protein Data Bank: 3KM9

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00392
  • symbol: SAOUHSC_00392
  • description: superantigen-like protein 7
  • length: 231
  • theoretical pI: 9.48067
  • theoretical MW: 26167.5
  • GRAVY: -0.649784

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Exotoxin 1
  • PFAM:
    OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 107.9)
    and 2 more
    Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 38.5)
    MAP; MAP domain (PF03642; HMM-score: 22.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: GQAVQAKE
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.98519
    • TAT(Tat/SPI): 0.002363
    • LIPO(Sec/SPII): 0.00867
    • Cleavage Site: CS pos: 30-31. VQA-KE. Pr: 0.8054
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIKDLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKDVFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEVSLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNSKDINKIEVTLKQI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR* (activation) regulon
    SaeR*(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

R J Williams, J M Ward, B Henderson, S Poole, B P O'Hara, M Wilson, S P Nair
Identification of a novel gene cluster encoding staphylococcal exotoxin-like proteins: characterization of the prototypic gene and its protein product, SET1.
Infect Immun: 2000, 68(8);4407-15
[PubMed:10899837] [WorldCat.org] [DOI] (P p)Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)Jovanka Bestebroer, Piet C Aerts, Suzan H M Rooijakkers, Manoj K Pandey, Jörg Köhl, Jos A G van Strijp, Carla J C de Haas
Functional basis for complement evasion by staphylococcal superantigen-like 7.
Cell Microbiol: 2010, 12(10);1506-16
[PubMed:20545943] [WorldCat.org] [DOI] (I p)

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