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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00539
  • pan locus tag?: SAUPAN002329000
  • symbol: SAOUHSC_00539
  • pan gene symbol?: dck
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00539
  • symbol: SAOUHSC_00539
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 543908..544570
  • length: 663
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAATTACGGTATTCCACAAAATGCCATTATAACCATTGCAGGTACAGTTGGTGTT
    GGAAAATCAACACTAACGCAAGCACTTGCAGATAAATTAAACTTTAAAACGTCTTTTGAA
    AATGTCGAACATAATCCATATTTAGATAAATTTTACAGCGATTTTGAACGATGGAGTTTC
    CATTTGCAAATTTACTTCTTAGCTGAACGTTTTAAAGAACAAAAGCGTATGTTTGAATAT
    GGTGGTGGCTTTGTCCAAGATCGATCAATTTATGAAGATGTTGATATTTTTGCAAAAATG
    CATGAAGAAGAAGGCACAATGAGTAAAGAAGATTTCAAAACATATTCAGACTTATTTAAT
    GCCATGGTCATGACACCTTATTTTCCTAAACCTGATGTAATGATTTATTTAGAATGTAAC
    TATGATGAGGTCATTGATCGTATTATTGAACGTGGTCGCGAAATGGAAATTAATACAGAC
    CCTGAATACTGGAAAAAGCTATTTAAACGCTATGACGATTGGATTAATAGCTTTAATGCA
    TGTCCAGTTGTACGTATCAATATTAATGAATATGATATCCATAAGGACCCCGAATCTTTA
    AATCCTATGATAAACAAAATTGCTCGAATTATTCAAACATATCGACAAGTAGATACACGA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    663

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00539
  • symbol: SAOUHSC_00539
  • description: hypothetical protein
  • length: 220
  • theoretical pI: 4.80408
  • theoretical MW: 26227.5
  • GRAVY: -0.58

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsdTMP kinase (TIGR00041; EC 2.7.4.9; HMM-score: 31.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 21.2)
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 18.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPantothenate and coenzyme Adephospho-CoA kinase (TIGR00152; EC 2.7.1.24; HMM-score: 18.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionscytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 17.8)
    Genetic information processingProtein synthesistRNA aminoacylationL-seryl-tRNA(Sec) kinase (TIGR03574; EC 2.7.1.164; HMM-score: 17.5)
    Unknown functionGeneralsmall GTP-binding protein domain (TIGR00231; HMM-score: 17)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotidesuridine kinase (TIGR00235; EC 2.7.1.48; HMM-score: 15.1)
    MetabolismCentral intermediary metabolismSulfur metabolismadenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 14.1)
    nicotinamide-nucleotide adenylyltransferase (TIGR01526; EC 2.7.7.1; HMM-score: 13.9)
    UMP-CMP kinase family (TIGR01359; EC 2.7.4.-; HMM-score: 13.5)
    carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 13)
    adenylate kinase (TIGR01360; EC 2.7.4.3; HMM-score: 12.6)
    MetabolismTransport and binding proteinsUnknown substrateenergy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 12.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsadenylate kinase (TIGR01351; EC 2.7.4.-; HMM-score: 11.9)
    ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 11.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllmagnesium chelatase ATPase subunit D (TIGR02031; EC 6.6.1.1; HMM-score: 10.1)
  • TheSEED:  
    Purine conversions Deoxyadenosine kinase (EC 2.7.1.76) / Deoxyguanosine kinase (EC 2.7.1.113) 
  • PFAM:
    P-loop_NTPase (CL0023) dNK; Deoxynucleoside kinase (PF01712; HMM-score: 211.6)
    AAA_18; AAA domain (PF13238; HMM-score: 46.1)
    Thymidylate_kin; Thymidylate kinase (PF02223; HMM-score: 23.9)
    AAA_17; AAA domain (PF13207; HMM-score: 22.8)
    AAA_15; AAA ATPase domain (PF13175; HMM-score: 20.4)
    AAA_28; AAA domain (PF13521; HMM-score: 20.2)
    Cytidylate_kin2; Cytidylate kinase-like family (PF13189; HMM-score: 18)
    ABC_tran; ABC transporter (PF00005; HMM-score: 17.7)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 17.6)
    AAA_22; AAA domain (PF13401; HMM-score: 17)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 16.9)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 15.4)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 14.8)
    AAA_33; AAA domain (PF13671; HMM-score: 14.6)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 14.5)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.3)
    ATPase; KaiC (PF06745; HMM-score: 14.2)
    SKI; Shikimate kinase (PF01202; HMM-score: 14.1)
    AAA_14; AAA domain (PF13173; HMM-score: 14)
    Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 13.7)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 13.3)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.3)
    PduV-EutP; Ethanolamine utilisation - propanediol utilisation (PF10662; HMM-score: 12.5)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 12.4)
    GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 12.3)
    NACHT; NACHT domain (PF05729; HMM-score: 12.3)
    no clan definedDUF1087; Domain of Unknown Function (DUF1087) (PF06465; HMM-score: 12.3)
    P-loop_NTPase (CL0023) NTPase_1; NTPase (PF03266; HMM-score: 12.2)
    AAA_25; AAA domain (PF13481; HMM-score: 12.1)
    APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 11.8)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 11.8)
    ParA; NUBPL iron-transfer P-loop NTPase (PF10609; HMM-score: 11.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.4
    • Ymax_pos: 31
    • Cmax: 0.387
    • Cmax_pos: 31
    • Smax: 0.62
    • Smax_pos: 28
    • Smean: 0.295
    • D: 0.359
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEHNPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHEEEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTDPEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVDTR

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]