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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00547
  • pan locus tag?: SAUPAN002338000
  • symbol: SAOUHSC_00547
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00547
  • symbol: SAOUHSC_00547
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 555273..556745
  • length: 1473
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAATTACATTTTAGGAACAATTTTAGAAAGTAAAATTACAGGTGTAGAAAAAGCGCAA
    ATAAATAGATTGAAGTTGTTCAAACAACACGGCATATCTTCAAAATGTGTATATGTTAAA
    TGGAATCCTTATTCATACACATATGCGAAGCAACATCAGATTGAAAATGATGTATTTACA
    ATGTATGACTATTTTCAAAAAGCAATCAATTATAAAAAGACAAAGCAAGTTAACTGGATA
    CAGTATTGGGAAAAGTCATGTAGGTACACATTGAAATTTGTGGAAAATTCAAATGATGTC
    AGAATATATGATGAAGAGCAATTTGTAATGTATGCTCATTTTTTAGATAAACAGTATCAT
    CAATTAAATTATGTGAATTATTTTGATCATAAAAGAAGAAAAGTAAAACGCGAATTGTAT
    GATGGAAGAGGCTTTTTAAGTTGTTCTCGAATTTTAGGTGAAGGACAACGGATTGTACTC
    GAAAATTACTATACACCTAATGGGGAAATCGTCATCCAAAAATATTTTGACGATATAAAA
    GGGAAAAACACGCTCACAAAGGTTATCTTAAATGAAGACCAGCATCAACAATTTTTTGAT
    ACAGAAGATGAATTAGTTCAATATTTTCTCCATCAATTATGTAAAAATAATGATCAAATC
    ATATTAGATCGTCCTCATGAATTAGGAAATGTTATAGCGGGATTAAATCAAAGTATTCCA
    GTTATTGTTGTGCTCCATAGTACACATTTATCCGGTGCCGGTAATGGTATAAAAAGTTTT
    TATAAAACAGTGTTTAATAATTTAACACGTTATAAAGCGATTGTTGTATCAACAGAAAAG
    CAATGCCAAGATATTTCACAATATATTGAAAATAAAATACCAGTTATCAATATTCCGGTT
    GGCTACGTGGCAAATTTAAAGTATCAATTTGACATCAATCAAAAGGAGAAAAATCATATC
    ATATCAATTGCTCGCCTCGTTGAAAATAAACAAATTAAACATCAAATTGAAGTAATCAAG
    CAATTAGTAACAAAACATCCCAATATTCAATTGAATATTTATGGACATGGAAATGGTTTG
    TCAGAATATCGACAACTTGTAGAAGATTATCATTTATCGGAACATGTTAAATTTCATGGT
    TTTAAGACGCATATTAATGAAGAGATTGCTAAAGCAGAACTGATGTTATCGACAAGTAAA
    ATGGAAGGTTTTGGCTTAGCAATTTTAGAGTCGCTTTCAGTAGGTACACCAGTGATCAGT
    TATGATGTAGATTATGGTCCATCAGAACTGATTCAAGATGGATTTAATGGCTATTTAGTA
    CCTCAAGGTGACATCAATCAAATGGTTGAAAAGGTCGACCAATTACTAAATAATACTCAA
    AAATTGCAACAGTTTTCAATTAATAGCATAGAATCTGCACAACAGTACAATGCAACTACT
    ATCAGTACAAAGTGGCAAAATATTTTAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1473

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00547
  • symbol: SAOUHSC_00547
  • description: hypothetical protein
  • length: 490
  • theoretical pI: 7.96323
  • theoretical MW: 57192.6
  • GRAVY: -0.473469

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein modification and repairaccessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 151.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsN-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 105.2)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 70.3)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 64.1)
    putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 49.7)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 38)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 36.5)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 35.4)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 35.4)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 30)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 26.6)
    Unknown functionEnzymes of unknown specificityglycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 26.3)
  • TheSEED:  
    Cell Wall and CapsuleGram-Positive cell wall componentsTeichoic and lipoteichoic acids biosynthesis Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52) 
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 132.2)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 85.8)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 26.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.178
    • Ymax_pos: 20
    • Cmax: 0.189
    • Cmax_pos: 20
    • Smax: 0.2
    • Smax_pos: 18
    • Smean: 0.157
    • D: 0.17
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPYSYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRIYDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVLENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNNDQIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVVSTEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKHQIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKAELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVEKVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]