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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00615
  • pan locus tag?: SAUPAN002478000
  • symbol: SAOUHSC_00615
  • pan gene symbol?:
  • synonym:
  • product: haloacid dehalogenase-like hydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00615
  • symbol: SAOUHSC_00615
  • product: haloacid dehalogenase-like hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 607070..607789
  • length: 720
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGATTTGAATCAAATTAAAGCAGTTGTATTTGATTTAGAAGGTACGTTGTTGGACAGA
    GTTAAATCTCGAGAGAAATTTATCGAAGAGCAATATGAACGATTTCATGACTACTTAATT
    CATGTTCAACTGGCAGATTTTAAAAAAGCATTTATTGAGCTAGATGACGATGAAGATAAT
    GATAAACCTGATTTATATAAAGAAATCATTAAACGTTTCCATGTAGATAGGTTAACTTGG
    AAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGAT
    ACTTTGTATACACTAGAAAAGCTATCGCAAAAAGGCTTTCAAATTGGTGTTATCGCAAAT
    GGTAAATCTAAGATTAAACAATTTCGATTACATTCACTTGGTTTGATGCATGTTATTAAT
    TATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATCCTAAAATTTTTGAAGAT
    ATGATTGATCAACTAGGGGTATTACCTGAGCAAATTATGTATGTTGGCGATGATGCGTTA
    AATGATGTAGCTCCAGCACGAGCTATGGGCATGGTTAGTGTATGGTATAAACAAGAAGAT
    GCTGAAATTGAACCACTCGAAGAAGAAGTTGATTTTACAATTACAACAGTGGAAGAATTA
    TTAACCATTTTACCAATAAAAAATGATAATAAAGGAGAAAATTATGGATCTATTTACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00615
  • symbol: SAOUHSC_00615
  • description: haloacid dehalogenase-like hydrolase
  • length: 239
  • theoretical pI: 4.54953
  • theoretical MW: 28196.9
  • GRAVY: -0.401255

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 125.6)
    HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 80.2)
    noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 77.8)
    haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 70.5)
    MetabolismEnergy metabolismSugarsphosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 61.4)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIIA (TIGR01662; HMM-score: 52.4)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 50.8)
    AHBA synthesis associated protein (TIGR01454; HMM-score: 50.3)
    epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 48.2)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 40.3)
    beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 39)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 35)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 32.7)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesbeta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 31.1)
    pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 27.1)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIA (TIGR01460; HMM-score: 26.4)
    HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 25.5)
    HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 23.3)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 22.6)
    MetabolismCentral intermediary metabolismOtherphosphonoacetaldehyde hydrolase (TIGR01422; EC 3.11.1.1; HMM-score: 17.9)
    phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 17.1)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharides3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 16.7)
    HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 16)
    HAD phosphatase, family IIIC (TIGR01681; HMM-score: 14.1)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 14)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, TIGR01457 family (TIGR01457; HMM-score: 12.3)
  • TheSEED:  
    Housecleaning nucleoside triphosphate pyrophosphatases L-2-haloalkanoic acid dehalogenase 
  • PFAM:
    HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 99.4)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 56.2)
    Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 33.6)
    PNK3P; Polynucleotide kinase 3 phosphatase (PF08645; HMM-score: 19)
    PGP_phosphatase; Mitochondrial PGP phosphatase (PF09419; HMM-score: 17.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.104
    • Ymax_pos: 28
    • Cmax: 0.121
    • Cmax_pos: 24
    • Smax: 0.146
    • Smax_pos: 22
    • Smean: 0.082
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQLADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTLYTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFEDMIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVEELLTILPIKNDNKGENYGSIY

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]