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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00633
  • pan locus tag?: SAUPAN002502000
  • symbol: SAOUHSC_00633
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00633
  • symbol: SAOUHSC_00633
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 621143..623146
  • length: 2004
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    GTGATACTAAGTTCGTTTGTCCATACTTTCATACCTAAAGTACCCCTAGCATTTATACAA
    ATTTTCTTGGGCATGTTACTATTTATTACCCCAATCCCTGTTCAATTTAATTTTGATTCT
    GAATTGTTTATGGTAACAATGATTGCGCCTTTGTTATTTGTAGAAGGTGTTAATGTTTCT
    AGAGTCCATTTAAGGAAATATATTAAGCCAGTGATGATGATGGCATTAGGATTAGTCATT
    ACTACTGTGATAGGTGTAGGTTTATTTATTCATTGGATTTGGCCAGATTTACCTATTGGA
    GCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATC
    ACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTG
    AATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCT
    TTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTT
    GGTTTACTTATAGGTATGGCATTAGTAAGGTTCCGATTAACATTGATGCGTCGAGGATAT
    GAAAACATTAATATGTTTACAATTATTCAATTGTTAACACCATTTGTTACGTATTTAATT
    GCTGAATTGTTTCACGCATCAGGAATCATTGCAGCAGTAGTTGCAGGACTTGTACATGGT
    TTCGAACGTGACAGAATTATGCAAGTACGTACACAACTGCAAATGAGTTACAATCATACA
    TGGAATATACTAGGTTATGTTTTAAATGGCTTTGTTTTTTCAATATTAGGATTTTTAGTA
    CCTGAAGTTATTATTAAAATTATCAAAACAGAACCGCACAATTTAATCTTTTTAATAGGC
    ATCACTATTGTTGTTGCTTTAGCTGTCTATCTATTTAGATTTGTTTGGGTTTATGTCTTA
    TATCCTTATTTTTATTTAGCCATCAGTCCATTCCAAAAAATGATGACTAAAAATGATGAT
    GATAATCCAACGACTGAGAAACCACCAAAGCGAAGTTTATACGCTTTAATTATGACGTTA
    TGTGGTGTGCATGGAACAATTTCTTTAGCAATCGCATTAACGTTACCGTATTTTTTAGCA
    GGGCATCATGCTTTTACGTATAGAAACGACTTATTATTTATTGCATCTGGTATGGTTATT
    ATTAGTTTGGTAGTTGCGCAAGTATTATTGCCATTATTAACGAAACCTGCACCTAAAACA
    GTAATTGGCAATATGTCGTTTAAAGTTGCTAGAATTTATATATTAGAACAAGTTATTGAT
    TATCTAAATCAAAAATCTACTTTCGAAACAAGTTTTAAATATGGTAACGTGATTAAAGAA
    TATCATGATAAATTAGCATTTTTAAAAACTGTAGAGAAAGATGATGAAAACTCTAAAGAA
    TTAGAACGTCTACAAAAAATTGCTTTTAATGTAGAAACAAAAACATTAGAGTCTTTAGTA
    GATGAAGGACAAATAACGAATAGTGTACTTGAAAACTATATGCGTTATGCTGAAAGAACA
    CAGGTATATAGACAAGCATCATTAATAAGAAGAATGATTGTATTATTACGAGGTGCTTTA
    TTAAAACGAAGAGTACAAACGAGAGTGAACTCCGCATCTTCACTTAGTGTTACGGATAAC
    TTAATGGAATTAAATAAAATTAATAAATTAGTCCATTATAATGTGGTTAGTCGTTTGTCT
    AAGGAAACAACAAAAGATAATACACTTGAAGTTGGAATGGTTTGTGACGGTTATTTAATG
    CGAATTGAAAACTTAACACCATCAAATTTCTTCAACTCAGCAAGTGAAGATACGATTACT
    AAAATTAAATTAAATGCATTGAGAGAACAACGTCGCATTTTACGTGAGTTGATTGATACA
    GATGAAGTATCAGAAGGTACAGCGTTAAAACTAAGAGAAGCCATCAATTATGATGAAATG
    GTTATTGTAGATAGTATGACGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2004

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00633
  • symbol: SAOUHSC_00633
  • description: hypothetical protein
  • length: 667
  • theoretical pI: 9.23772
  • theoretical MW: 74934.3
  • GRAVY: 0.471664

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsNa+/H+ antiporter (TIGR00831; HMM-score: 258)
    MetabolismTransport and binding proteinsCations and iron carrying compoundssodium/hydrogen exchanger 3 (TIGR00840; HMM-score: 52.1)
    MetabolismTransport and binding proteinsCations and iron carrying compoundssodium/hydrogen antiporter (TIGR00844; HMM-score: 7.3)
  • TheSEED:  
    Na+/H+ antiporter 
  • PFAM:
    CPA_AT (CL0064) Na_H_Exchanger; Sodium/hydrogen exchanger family (PF00999; HMM-score: 173.4)
    no clan definedTrans_coact; Phage late-transcription coactivator (PF16805; HMM-score: 14.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.164
    • Ymax_pos: 11
    • Cmax: 0.169
    • Cmax_pos: 35
    • Smax: 0.318
    • Smax_pos: 2
    • Smean: 0.268
    • D: 0.204
  • predicted transmembrane helices (TMHMM): 13

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MILSSFVHTFIPKVPLAFIQIFLGMLLFITPIPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLFIHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGIISFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINMFTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNILGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLYPYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFLAGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQVIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKTLESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSASSLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFFNSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]