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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00823
  • pan locus tag?: SAUPAN002806000
  • symbol: SAOUHSC_00823
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00823
  • symbol: SAOUHSC_00823
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 803182..803370
  • length: 189
  • essential: no [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACG
    TTTATACTCGTATTAATGAAAAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGT
    TTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAATATACACATTTATATTTGGT
    GTGCTATAA
    60
    120
    180
    189

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00823
  • symbol: SAOUHSC_00823
  • description: hypothetical protein
  • length: 62
  • theoretical pI: 10.0084
  • theoretical MW: 7172.78
  • GRAVY: 1.45968

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    FIG01107975: hypothetical protein 
  • PFAM:
    no clan definedBRI3BP; Negative regulator of p53/TP53 (PF14965; HMM-score: 16.3)
    GPCR_A (CL0192) Bac_rhodopsin; Bacteriorhodopsin-like protein (PF01036; HMM-score: 15)
    no clan definedDUF3611; Protein of unknown function (DUF3611) (PF12263; HMM-score: 13.5)
    Intg_mem_TP0381; Integral membrane protein (intg_mem_TP0381) (PF09529; HMM-score: 13.4)
    DUF4051; Protein of unknown function (DUF4051) (PF13260; HMM-score: 12.2)
    PGG; Domain of unknown function (PF13962; HMM-score: 12.1)
    SLC3A2_N; Solute carrier family 3 member 2 N-terminus (PF16028; HMM-score: 12.1)
    DUF3742; Protein of unknown function (DUF3742) (PF12553; HMM-score: 12)
    SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 11.9)
    Glucos_trans_II; Glucosyl transferase GtrII (PF14264; HMM-score: 11.7)
    MRAP; Melanocortin-2 receptor accessory protein family (PF15183; HMM-score: 11)
    TEX29; Testis-expressed sequence 29 protein (PF15839; HMM-score: 10.8)
    DUF3671; Protein of unknown function (PF12420; HMM-score: 10.5)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 9.9)
    no clan definedCcmH; Cytochrome C biogenesis protein (PF03918; HMM-score: 9.7)
    NfeD-like (CL0252) NfeD; NfeD-like C-terminal, partner-binding (PF01957; HMM-score: 9.6)
    no clan definedDUF2208; Predicted membrane protein (DUF2208) (PF09973; HMM-score: 9.4)
    DHHC; DHHC palmitoyltransferase (PF01529; HMM-score: 8.7)
    Peptidase_MA (CL0126) DUF3810; Protein of unknown function (DUF3810) (PF12725; HMM-score: 8.4)
    no clan definedTmemb_55A; Transmembrane protein 55A (PF09788; HMM-score: 6)
    DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 4.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.186
    • Ymax_pos: 33
    • Cmax: 0.196
    • Cmax_pos: 33
    • Smax: 0.377
    • Smax_pos: 3
    • Smean: 0.191
    • D: 0.188
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]