From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00875
  • pan locus tag?: SAUPAN003037000
  • symbol: SAOUHSC_00875
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00875
  • symbol: SAOUHSC_00875
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 839357..840421
  • length: 1065
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAACTTAGTTTTGTTAGGCGGCGGATATGGTAATATGCGTATCATGTCACGCATT
    TTAACTACTTCTTTACCACAAGATTATACAGTCACATTAGTTGATCGTATGCCATTTCAT
    GGATTGAAACCAGAATTTTATGCTTTAGCTGCGGGCACGAAATCAGATAAAGATGTTCGT
    ATGAAATTCCCTAATCATCCACAAGTGAATACAGTTTATGGTGAAATTAACGACATAGAT
    TTAGATGCTCAAATTGTCTCAGTCGGTAATTCTAAAATTGATTATGATGAGCTAATCATT
    GGTTTAGGATGTGAAGATAAATATCATAACGTTCCAGGAGCCGAAGAATATACACATAGT
    ATTCAAACACTCTCAAAGGCTCGGGATACTTTCCATAGTATTAGTGAACTACCAGAAGGT
    GCTAAAGTCGGTATCGTTGGTGCTGGATTAAGCGGCATAGAACTTGCCAGCGAATTAAGA
    GAAAGTAGATCAGACTTGGAAATATATCTTTATGACCGTGGGCCGCGAATTTTAAGAAAT
    TTTCCAGAAAAATTAAGTAAGTATGTTGCGAAATGGTTCGCCAAAAATAATGTTACCGTT
    GTTCCAAATTCAAATATTAATAAAGTTGAACCTGGTAAAATATATAACTGTGATGAACCT
    AAAGATATTGATTTAGTTGTATGGACAGCAGGAATTCAACCTGTTGAAGTTGTTCGTAAC
    TTGCCGATTGATATAAATAGTAATGGACGCGTGATAGTTAACCAGTATCATCAAGTACCA
    ACATATCGTAACGTCTATGTAGTTGGTGATTGTGCTGATTTACCACATGCGCCAAGTGCT
    CAGTTAGCCGAAGTTCAAGGTGATCAAATTGCCGATGTGCTTAAAAAGCAATGGCTAAAT
    GAACCATTACCTGACAAAATGCCGGAACTAAAGGTACAAGGTATCGTTGGGTCATTAGGA
    GATAAGCAAGGTTTTGCATATATCATGGACCGCACTGTAACAGGACGACTCGCTTCTATT
    TTAAAATCCGGCGTACTTTGGTTATATAAATATCATAATGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1065

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00875
  • symbol: SAOUHSC_00875
  • description: hypothetical protein
  • length: 354
  • theoretical pI: 6.31904
  • theoretical MW: 39398.7
  • GRAVY: -0.272034

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 107.7)
    Cellular processesCellular processesDetoxificationCoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 70)
    oxidoreductase/SelD-related fusion protein (TIGR04369; HMM-score: 60.9)
    MetabolismEnergy metabolismElectron transportglutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 57.6)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 55)
    MetabolismCentral intermediary metabolismNitrogen metabolismnitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 45.5)
    Cellular processesCellular processesDetoxificationmercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 42.5)
    MetabolismEnergy metabolismElectron transportglutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 32)
    trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 20.1)
    Cellular processesCellular processesDetoxificationalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 14.3)
    Cellular processesCellular processesAdaptations to atypical conditionsalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 14.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprotoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 14.1)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 13)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 11.6)
  • TheSEED:  
    RespirationElectron donating reactionsRespiratory dehydrogenases 1 NADH dehydrogenase (EC 1.6.99.3) 
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 134)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 49.4)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 25.3)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 20.7)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 19.5)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 18.2)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 17.1)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 14.8)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.159
    • Ymax_pos: 13
    • Cmax: 0.15
    • Cmax_pos: 51
    • Smax: 0.332
    • Smax_pos: 6
    • Smean: 0.25
    • D: 0.194
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKPEFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGLGCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELRESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCDEPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPHAPSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVTGRLASILKSGVLWLYKYHNG

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_00875 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]