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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00879
  • pan locus tag?: SAUPAN003041000
  • symbol: SAOUHSC_00879
  • pan gene symbol?: pepZ
  • synonym:
  • product: cytosol aminopeptidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00879
  • symbol: SAOUHSC_00879
  • product: cytosol aminopeptidase
  • replicon: chromosome
  • strand: +
  • coordinates: 843139..844614
  • length: 1476
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATA
    CCAGAACATTTAAATCAGTTAGAGCGCATTAGTTTTAATCATATCGATATTACAGAATCA
    CTTGAAAGACTAAAACATCAACATATTATTGGTAGTAAAGTTGGGAAGATTTATACAACT
    GCATTTGATGTACAAGATCAAACATATCGTTTAATTACAGTTGGTTTAGGAAACTTAAAG
    ACACGTAGTTATCAAGATATGTTGAAAATATGGGGACATCTTTTCCAATACATAAAGTCA
    GAACACATTGAAGATACGTATTTACTTATGGATTCATTTATTTCAAAATATGATCAGTTA
    TCAGATGTATTAATGGCATGCGGTATTCAAAGTGAGCGTGCAACATATGAATTCGATCAT
    TATAAATCAAGTAAGAAGGCACCGTTTAAGACGAATTTAAACCTTATTAGTGAATCATTA
    ATTGAATTAGATTTTATTCATGAGGGTATCAGTATTGGCCAATCCATTAATTTGGCAAGA
    GACTTTAGTAATATGCCACCGAATGTATTAACACCACAAACATTTGCAGAAGATATTGTT
    AATCATTTTAAAAATACAAAGGTCAAAGTAGATGTTAAAGATTATGACACTTTAGTTTCT
    GAAGGATTCGGACTTTTACAAGCAGTAGGTAAAGGTAGTAAGCATAAACCGAGATTAGTA
    ACCATCACATATAATGGCAAAGACAAAGATGAAGCACCAATTGCCTTAGTTGGTAAAGGT
    ATAACGTATGATTCTGGTGGTTATAGTATTAAAACGAAGAATGGCATGGCTACAATGAAG
    TTTGACATGTGTGGCGCTGCGAATGTCGTTGGTATCATTGAAGCGGCTAGTCGTTTACAA
    CTGCCTGTAAATATTGTCGGAGTGCTTGCGTGTGCTGAAAATATGATAAATGAAGCATCA
    ATGAAGCCAGATGATGTATTTACAGCATTAAGTGGTGAAACTGTAGAAGTAATGAATACA
    GACGCTGAAGGTAGATTAGTCCTTGCAGATGCTGTGTTTTATGCAAATCAATATCAGCCT
    AGTGTGATTATGGACTTTGCTACATTAACGGGTGCAGCAATTGTTGCACTAGGCGATGAT
    AAAGCTGCTGCATTTGAATCGAATAGTAAAGTGATATTAAACGATATATTACAAATAAGT
    TCTGAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATT
    AAACACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTT
    GCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCA
    GGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATG
    ATTCCGACGATAGTACAATGGTTAAAACAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1476

Protein[edit source | edit]

Protein Data Bank: 3KZW

General[edit source | edit]

  • locus tag: SAOUHSC_00879
  • symbol: SAOUHSC_00879
  • description: cytosol aminopeptidase
  • length: 491
  • theoretical pI: 5.71046
  • theoretical MW: 54128.3
  • GRAVY: -0.116904

Function[edit source | edit]

  • reaction:
    EC 3.4.11.1?  ExPASy
    Leucyl aminopeptidaseRelease of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low
  • TIGRFAM:
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismDehydrogenase complexes Cytosol aminopeptidase PepA (EC 3.4.11.1) 
    Protein MetabolismProtein degradationEC 3.4.11.- Aminopeptidases Cytosol aminopeptidase PepA (EC 3.4.11.1) 
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M17; Cytosol aminopeptidase family, catalytic domain (PF00883; HMM-score: 358.8)
    MACRO (CL0223) Peptidase_M17_N; Cytosol aminopeptidase family, N-terminal domain (PF02789; HMM-score: 61.5)
    no clan definedTerD; TerD domain (PF02342; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.135
    • Ymax_pos: 12
    • Cmax: 0.11
    • Cmax_pos: 30
    • Smax: 0.197
    • Smax_pos: 8
    • Smean: 0.178
    • D: 0.152
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERLKHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEHIEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESLIELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTLVSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGMATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGETVEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVILNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSGQTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]