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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00882
  • pan locus tag?: SAUPAN003046000
  • symbol: SAOUHSC_00882
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00882
  • symbol: SAOUHSC_00882
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 846790..847944
  • length: 1155
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCATTGGACAATTATCGGCGGTGGCATACAGGGAACTGCAATCGCACAAAAACTATTA
    TCAAGCGGATTAACAACAGACCGATTAACAATCATTGACCCACACGAAACTTTTTGCCAA
    AGGTTTAACTCATATACAAATCGAATAGAAATGCCTTATTTAAGATCACCGATTGTACAT
    CACGTACATCCACAACCGTTCCATCTAAAACAATTCGCTAAACAGCACCAATATACAAAT
    GCTTTTTATGGTCCTTATCAACGACCTGAATTGACAATGTTTATGGATCATATTGCACAT
    GCTTCTAAACAATATCAATTAGAGGATTGCTTGGTTCAAGGTTTAGTTCAAACTTTAGAT
    AAACAAGAAGACAAATGGCATATCAAGTTAGAAGATGGACAAATTATCACTACAGATTGC
    GTCGTTATTGCAATAGGCAGTACAAATATTCCGTTTATGCCTGACATTTTAAAAGACAAA
    CAGAATGTAAATCATATCTTCGAGAAAGAACTTGATCAAGTAGTATATGATAAGACCGAT
    CATATCGTTGGTAGCGGCATTACTGCTGCACATCTTGCACTTAAATTGTTAAATCATGAT
    AACGATAAAAAGATTCATTTATGGCTAAATAAAGATATTGAAATACATGACTTTGATGCT
    GATCCTGGTTGGTTAGGTCCGAAAAATATGTCTTCATTTTTAAGTACTAAAAGCATGCCT
    GAAAGAAATGCCATTGTACAACGCGAACGTCATAAAGGATCAATGCCTCACGAACTGTAC
    TTACGCCTTAAAAAACATATTAAAAATGGTCGTATAAATGTGCATAAAACACCTATCACT
    CAAATTAGTGGTGGTGTAATTAACACTGAAAATGATTCTGTTCCATATCAACAGATTATG
    GTTGCAACTGGTTTTGAACAAGATTTTATGTCACAACCACTTATTAAGCAATTAATACAA
    AATTATGATGCACCTATCAACGAATGTAATTACCCTGTTATTTCCGAAAAATTAGAATGG
    ATACCAAATCTATTTGTCGCAGGATGCTTTGCAGACTTAGAATTAGGACCATTTGGTAGA
    AATGTTATGGGTGGCCGTAAAGCTGCCGAACGCATTGAACAAGCATTTCTAAAACTACAA
    CAATATAGCGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1155

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00882
  • symbol: SAOUHSC_00882
  • description: hypothetical protein
  • length: 384
  • theoretical pI: 7.07664
  • theoretical MW: 43955
  • GRAVY: -0.403906

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 30.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 25.3)
    lycopene cyclase family protein (TIGR01790; HMM-score: 21.5)
    putative histamine N-monooxygenase (TIGR04439; EC 1.14.13.-; HMM-score: 16.3)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 13.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 13.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamineglycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 12.3)
    Cellular processesCellular processesDetoxificationalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 12.1)
    Cellular processesCellular processesAdaptations to atypical conditionsalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 12.1)
    Unknown functionEnzymes of unknown specificityflavoprotein, HI0933 family (TIGR00275; HMM-score: 11.7)
    Cellular processesCellular processesBiosynthesis of natural products2,3-diaminopropionate biosynthesis protein SbnB (TIGR03944; HMM-score: 11)
  • TheSEED:  
    FIG01108091: hypothetical protein 
  • PFAM:
    NADP_Rossmann (CL0063) NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 52.2)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 45.4)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 43.5)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 41.3)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 22.6)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 22.3)
    no clan definedMAF_flag10; Protein of unknown function DUF115 (PF01973; HMM-score: 21.3)
    NADP_Rossmann (CL0063) Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 18.1)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.1)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 15.7)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 14.4)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.128
    • Ymax_pos: 24
    • Cmax: 0.135
    • Cmax_pos: 17
    • Smax: 0.183
    • Smax_pos: 36
    • Smean: 0.111
    • D: 0.121
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNSYTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHASKQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDKQNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDFDADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHKTPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVISEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]