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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00913
  • pan locus tag?: SAUPAN003114000
  • symbol: SAOUHSC_00913
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00913
  • symbol: SAOUHSC_00913
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 885304..886173
  • length: 870
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAATTTAGATTGGTACTATACATTTGTCATTTTAGCGAAGACGTTAAACTATCGATTA
    GCTAGTGAAGAAATTAATCTCACTATACCTTCTATACATAAGCAAATTAAAAATTTAGAA
    CAGCATCTCAATGTGAAACTATTTGAAACTTATAAAAATCAAATTATCTTGACTGAAGAT
    GGTCATACATTTCTTCCAATTGCGCAAAGTTTTATTGAACAATATGAAAGTGGCATCAAA
    CATATCCAACTTAAAAAAACGATGTTTCAATCGAAATTAAATGTTGTCGTGTCTTCATAT
    ATCGCGACATTCATTATGCCCAAATTTTTAAAATCTTTCTTTAACGAGCATCCTTTTATC
    GATGTTTCTCTTCATGTCAAAAATGAAAATATTGAAAAAGATATTAATAATCATACGTAT
    GACATCGGGATTAGCAGAAATCAACCTAAACTAAGAGAAGTTCATTCTGAAAAAGTATGC
    GAAGGTAAAATTGTATTGATTGCTCCCAATAAAGAGAATAATCATCTTCTAACTGAGGCA
    TCTTTATTTGAAAAATACAAAATAATAAGTGATAACCATCCAGAATATTGGTCATCACTT
    AAAAATAATATTTTAAATATTTATGAAAAAGCACAATTTCTATCTATCAATGATGTGCAT
    ACTTCAATTAAATTAATCGAAATGAATCAAGGTATATCATTCCTGCCTCTTTATATAACA
    ACAAATAGTGATTACAATATTTCGGTTATTAACACGAAAATTTTACAAGCACCTATTTCA
    TTTACATATATATACAGCAAAAAAGAAAGCCCAGAAATATTGGTGTTTATTAAATCATTT
    AAAAAGTATATTGCCAATGAACAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    870

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00913
  • symbol: SAOUHSC_00913
  • description: hypothetical protein
  • length: 289
  • theoretical pI: 8.35837
  • theoretical MW: 33798.7
  • GRAVY: -0.229412

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionsaminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 64.5)
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 52.1)
    MetabolismEnergy metabolismOtherpca operon transcription factor PcaQ (TIGR02424; HMM-score: 49.5)
    Signal transductionRegulatory functionsDNA interactionspca operon transcription factor PcaQ (TIGR02424; HMM-score: 49.5)
    Signal transductionRegulatory functionsDNA interactionsD-serine deaminase transcriptional activator (TIGR02036; HMM-score: 47.5)
    Cellular processesCellular processesToxin production and resistancetranscriptional regulator, ArgP family (TIGR03298; HMM-score: 11.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtranscriptional regulator, ArgP family (TIGR03298; HMM-score: 11.2)
    Signal transductionRegulatory functionsDNA interactionstranscriptional regulator, ArgP family (TIGR03298; HMM-score: 11.2)
  • TheSEED:  
    LysR family regulatory protein 
  • PFAM:
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 79.5)
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 43.7)
    SUKH (CL0526) SUKH_6; SMI1-KNR4 cell-wall (PF14568; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.127
    • Ymax_pos: 22
    • Cmax: 0.174
    • Cmax_pos: 22
    • Smax: 0.17
    • Smax_pos: 21
    • Smean: 0.093
    • D: 0.114
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLNVKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIATFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVCEGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSINDVHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVFIKSFKKYIANEQL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_00913 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]