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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00989
  • pan locus tag?: SAUPAN003254000
  • symbol: SAOUHSC_00989
  • pan gene symbol?: patA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00989
  • symbol: SAOUHSC_00989
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 960954..962108
  • length: 1155
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAACTTTCTTTAAATTCTAATTCTAAATATTTAAGAGCACCAAGTATTCGCCAATTT
    TCAAATCGTATGAATAATTTGGATGATTGTGTTAATTTAACTATTGGCCAACCAGACTTC
    CCGATGCCTGATGTTGTGAAAAAGGCATATATTGATGCTATTAACAATGACAAGACATCA
    TACTCTCACAATAAAGGGTTATTAGAAACTCGCGAAGCAATTAGTCAATACTTCAAAAAT
    CGTTATCATTTTTCCTATGATCCTGAAGAAATTATTGTGACAAATGGAGCAAGTGAAGCA
    ATAGACACAACGTTACGTTCTATCATAGAGCCTGGAGATGAAATTATAATACCGGGACCA
    ATTTATGCAGGCTACATACCACTCATCGAAGTACTAGGTGGTAAACCAATTTATATTGAT
    ACAACAGCAACACAATTTAAAATTACACCTGATGCATTAGAAAGTCATATTTCTCCAAAG
    ACAAAAGCTGTCTTGTTAAATTATCCGACTAATCCAACTGGCGTAGTTTTAAAAAGAAAT
    GAAGTTTTAAATATCGTAAATGTATTAAAAAAATATCCGTTATTTATTATTAGCGATGAG
    ATTTATGCTGAAAATACATTTAGTGGTAAACATGTATCCTTCGCTGAATTTGAAGACATT
    CGTGATCAATTAATATTAATTGGTGGTTTAAGTAAATCACACTCAGCAACCGGAATAAGA
    ATTGGTTTTCTATTAGGACCTCAATATTTGATTGATAAATTAACATTTATGCACGCCTAT
    AATTGTATTTTTGCCAATGTTCCAGCTCAAATAGCATGTATAACAGCACTTAACGAAGGA
    TTAGAAGCTCCTAAATATATGAACGAAGCTTATGTAGAACGAAGAAATTATTTAGTATCT
    GAATTAACTAAATTAGGTTTTGAGATTACCGCTCAACCTGAAGGCGCGTTTTATATTTTC
    CCAAGTATTAAACATATCACCGATGACGATTTCGAATTTTGTGTCGATTTACTTGAATCA
    ACACATTTAGCAATCGTTCCAGGCTCATCCTTTACAGAATTCGGTAAAGGATTTGTAAGG
    ATTTCCTATGCTTATGAAATGGATGTTTTAAAAGAAGGTATGAAGAGACTTGCTAAATAT
    TTAAATACTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1155

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00989
  • symbol: SAOUHSC_00989
  • description: hypothetical protein
  • length: 384
  • theoretical pI: 5.66657
  • theoretical MW: 43190.2
  • GRAVY: -0.115365

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 209.3)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 156.2)
    succinyldiaminopimelate transaminase (TIGR03538; EC 2.6.1.17; HMM-score: 137.3)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 135.8)
    MetabolismEnergy metabolismAmino acids and aminestyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 128.2)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 125.3)
    Cellular processesCellular processesBiosynthesis of natural productscapreomycidine synthase (TIGR03947; HMM-score: 124.1)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 123.4)
    MetabolismAmino acid biosynthesisHistidine familyhistidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 119.5)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 101.5)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 97.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminthreonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 84.9)
    enduracididine biosynthesis enzyme MppQ (TIGR04461; EC 2.-.-.-; HMM-score: 52.5)
    MetabolismEnergy metabolismAmino acids and aminesaspartate 4-decarboxylase (TIGR03801; EC 4.1.1.12; HMM-score: 41.1)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesTDP-4-keto-6-deoxy-D-glucose transaminase (TIGR02379; HMM-score: 28)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01977; HMM-score: 27.9)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 24.7)
    MetabolismEnergy metabolismAmino acids and aminesmethionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 24.4)
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 22.9)
    MetabolismAmino acid biosynthesisAspartate familyO-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 17.3)
    MetabolismAmino acid biosynthesisSerine familyO-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 17.3)
    aminotransferase, LLPSF_NHT_00031 family (TIGR04181; HMM-score: 16.4)
    MetabolismAmino acid biosynthesisAspartate familyO-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 15.5)
    MetabolismEnergy metabolismAmino acids and aminesglycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 13.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalamin5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 13.3)
    MetabolismAmino acid biosynthesisAspartate familycystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 12.9)
    Genetic information processingDNA metabolismRestriction/modificationcysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 12.9)
    MetabolismAmino acid biosynthesisAspartate familyO-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 12.6)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 12.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 11)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineLysine Biosynthesis DAP Pathway N-acetyl-L,L-diaminopimelate aminotransferase (EC 2.6.1.-) 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 212.1)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 39.9)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 35.2)
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 34.3)
    OKR_DC_1; Orn/Lys/Arg decarboxylase, major domain (PF01276; HMM-score: 18)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.218
    • Ymax_pos: 13
    • Cmax: 0.155
    • Cmax_pos: 49
    • Smax: 0.545
    • Smax_pos: 8
    • Smean: 0.472
    • D: 0.317
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPDVVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAIDTTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPKTKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFEDIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCIFANVPAQIACITALNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFCVDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]