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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01000
  • pan locus tag?: SAUPAN003268000
  • symbol: SAOUHSC_01000
  • pan gene symbol?: qoxC
  • synonym:
  • product: cytochrome c oxidase subunit III

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01000
  • symbol: SAOUHSC_01000
  • product: cytochrome c oxidase subunit III
  • replicon: chromosome
  • strand: -
  • coordinates: 972883..973488
  • length: 606
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAGTCATGATACAAACACTATTGATTCACGCACACATGAAGGCGAATTAAATAAGCTT
    GGCTTTTGGATTTTCATTACAGCCGAATTTGCGTTATTCGGTACCCTATTTGCGACACTA
    TTAACTTTGCAACATGGTGGCGATTATGCAGGTAAAATGACTACTGAGTTATTTGAATTG
    CCTCTTGTTTTAATAATGACGTTTGCATTATTATTCAGTTCTTACACATGTGGTATTGCT
    ATTTACTACATGCGCCAAGAAAAACAAAAGTTAATGATGTTTTGGATGATCATTACGTTA
    CTTTTAGGTTTAGTCTTTGTTGGATTCGAAATTTATGAATTCGCACACTATGCATCAGAA
    GGCGTTAACCCAACAATTGGTTCTTACTGGTCTAGTTTCTTTATCCTATTAGGAACGCAT
    GGTTGTCACGTATCGCTAGGTATCGTTTGGGCCATTTGTTTATTAATCCAAATCCAACGA
    CGCGGATTAGACAAATATAATGCGCCAAAATTATTTATAGTAAGTTTATACTGGCACTTC
    TTAGATGTTGTTTGGGTTTTCATCTTTACTGCCGTATATATGATAGGGATGGTGTATAGC
    GGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    606

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01000
  • symbol: SAOUHSC_01000
  • description: cytochrome c oxidase subunit III
  • length: 201
  • theoretical pI: 6.38351
  • theoretical MW: 23057
  • GRAVY: 0.666169

Function[edit source | edit]

  • reaction:
    EC 1.10.3.-?  ExPASy
    Ubiquinol-8 + O2 = Ubiquinone-8 + H2O?
  • TIGRFAM:
    MetabolismEnergy metabolismElectron transportcytochrome aa3 quinol oxidase, subunit III (TIGR02897; EC 1.10.3.-; HMM-score: 359)
    MetabolismEnergy metabolismElectron transportcytochrome o ubiquinol oxidase, subunit III (TIGR02842; EC 1.10.3.-; HMM-score: 188.3)
  • TheSEED:  
    Terminal cytochrome C oxidases AA3-600 quinol oxidase subunit IIII 
  • PFAM:
    no clan definedCOX3; Cytochrome c oxidase subunit III (PF00510; HMM-score: 51.6)
    STE2; Fungal pheromone mating factor STE2 GPCR (PF02116; HMM-score: 17.8)
    PsbL; PsbL protein (PF02419; HMM-score: 13.7)
    Yip1 (CL0112) DUF1129; Protein of unknown function (DUF1129) (PF06570; HMM-score: 6.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 51
    • Cmax: 0.15
    • Cmax_pos: 51
    • Smax: 0.137
    • Smax_pos: 45
    • Smean: 0.071
    • D: 0.097
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQHGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLLGLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQRRGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]