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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01032
  • pan locus tag?: SAUPAN003307000
  • symbol: SAOUHSC_01032
  • pan gene symbol?: cydB
  • synonym:
  • product: cytochrome d ubiquinol oxidase subunit II

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01032
  • symbol: SAOUHSC_01032
  • product: cytochrome d ubiquinol oxidase subunit II
  • replicon: chromosome
  • strand: +
  • coordinates: 1002135..1003154
  • length: 1020
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTTATGCATTTATAGGTATAACAGTGTTATGGTTGTTTTTATTTTGCTATATCATT
    ATTGCTTCTATAGATTTTGGGGCAGGTTTCTTCGCATTGCATTCAAAGTTAACTGGTGAT
    GAAAAGAAAATTAATCACTTAATTTCACGTTATTTAAACCCAGTTTGGGAAGTTACGAAT
    GTATTCTTTGTTTTCTTCTTCGTAGGATTCGTAGGTTTCTTTCCAGAGTCAATCAAATAT
    TTAGGCACGGTATTGTTAATACCAGGTTCAATAGCACTGATTATGATATCGTTGAGAAAC
    AGTTTTTATGCATTTGAAAATTATGGTCAAGATACAAAATTAGCATGGATGATCATGTAT
    GGGGTAAGTGGATTATTAATTCCAGCTTCATTATCTACTGCTTTAACTATTACAGAAGGT
    GGCTATATTAATGTTCGAAACAATGTTATCGATCTAGATTGGGTGCAGTTATTATTAAGC
    CCATTTGCTTGGTCTGTAGTATTCTTGGCAATTATTTCAGTTTTATATATTTCATCAGGA
    TTTTTGACATATTATGCTAAAAAAGCAAATGACGAACCAGCATATAATTTAACAAGACAA
    TGGCACATATTTTTAGGGCCGCCGATGATTATCATTTGTTTATTCGTATTTCTATCATTA
    CGCATTCAAAATTCTGAACATTTTTATTCAGCTGTTTTTGACTATTGGTGGATGTTTGGT
    ATAAGTTTCTTATTCTTTGCATTAGCTTCATTGTTAACATTCTTTAAGAAAAAACATGGT
    TTGGCTTTTGTATTTGTTATTTTACAAATGATGTTCGCGTTCTTTGGCTATGGAATTAGT
    AAATTGCCATACCTTTTATATCCGTTTGTAAAAATTACAGATGCATATGTTAATCCAGAA
    ATGGGCTGGACATTAGTGATTGTCTTTATTTTAGGTTTACTTTTATTACTTCCATCGTTA
    ATATTATTATTAAGATTATTTGTTTTCGACAAAGAATATGTTGAAGGAAAGAAATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01032
  • symbol: SAOUHSC_01032
  • description: cytochrome d ubiquinol oxidase subunit II
  • length: 339
  • theoretical pI: 8.52158
  • theoretical MW: 38946.1
  • GRAVY: 0.883481

Function[edit source | edit]

  • reaction:
    EC 1.10.3.-?  ExPASy
  • TIGRFAM:
  • TheSEED:  
    RespirationElectron accepting reactionsTerminal cytochrome d ubiquinol oxidases Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-) 
    Electron accepting reactionsTerminal cytochrome oxidases Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-) 
    Bacterial RNA-metabolizing Zn-dependent hydrolases Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-) 
    Conserved gene cluster associated with Met-tRNA formyltransferase Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-) 
  • PFAM:
    no clan definedCyt_bd_oxida_II; Cytochrome bd terminal oxidase subunit II (PF02322; HMM-score: 137.2)
    DUF2516; Protein of unknown function (DUF2516) (PF10724; HMM-score: 17.2)
    2heme_cytochrom (CL0328) Ni_hydr_CYTB; Prokaryotic cytochrome b561 (PF01292; HMM-score: 5.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 33
    • Cmax: 0.251
    • Cmax_pos: 23
    • Smax: 0.265
    • Smax_pos: 27
    • Smean: 0.098
    • D: 0.126
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKINHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSFYAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLSPFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFLSLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFGYGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYVEGKKS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]