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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01106
  • pan locus tag?: SAUPAN003390000
  • symbol: SAOUHSC_01106
  • pan gene symbol?: murI
  • synonym:
  • product: glutamate racemase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01106
  • symbol: SAOUHSC_01106
  • product: glutamate racemase
  • replicon: chromosome
  • strand: +
  • coordinates: 1068153..1068953
  • length: 801
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATAAACCAATAGGTGTAATAGACTCTGGTGTCGGAGGTTTGACAGTAGCTAAAGAA
    ATTATGCGTCAGTTGCCAAATGAGACGATTTATTACTTAGGTGATATTGGACGATGTCCA
    TATGGGCCAAGACCAGGAGAACAAGTAAAACAATATACAGTTGAAATCGCTCGTAAATTA
    ATGGAATTTGATATAAAAATGCTCGTGATTGCTTGTAATACAGCAACTGCTGTAGCTTTA
    GAATATTTACAAAAGACCTTATCAATCCCAGTGATTGGCGTAATTGAACCAGGTGCTAGA
    ACAGCAATAATGACTACTAGAAATCAAAATGTATTAGTACTAGGAACGGAAGGCACAATT
    AAATCTGAAGCATATCGTACGCATATTAAACGTATCAATCCACATGTAGAGGTACATGGC
    GTTGCCTGTCCAGGTTTTGTGCCACTTGTAGAACAAATGAGATATAGTGATCCAACAATT
    ACAAGCATTGTCATTCATCAAACACTGAAACGTTGGCGTAATAGTGAGTCTGATACTGTC
    ATTTTAGGATGTACCCACTATCCATTGCTCTATAAACCTATCTATGATTATTTTGGTGGT
    AAAAAGACAGTGATTTCGTCTGGATTAGAAACGGCTCGTGAAGTTAGTGCATTGCTAACA
    TTTAGTAATGAACATGCAAGTTATACTGAACATCCAGATCATCGATTTTTTGCAACAGGT
    GATCCTACTCACATTACTAACATTATCAAAGAGTGGTTAAATTTATCTGTCAATGTGGAA
    CGTATATCAGTGAATGACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    801

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01106
  • symbol: SAOUHSC_01106
  • description: glutamate racemase
  • length: 266
  • theoretical pI: 7.63246
  • theoretical MW: 29698
  • GRAVY: -0.120301

Function[edit source | edit]

  • reaction:
    EC 5.1.1.3?  ExPASy
    Glutamate racemaseL-glutamate = D-glutamate
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanglutamate racemase (TIGR00067; EC 5.1.1.3; HMM-score: 271.7)
    MetabolismEnergy metabolismAmino acids and aminesaspartate racemase (TIGR00035; EC 5.1.1.13; HMM-score: 51.1)
    Cellular processesCellular processesCell divisionseptum site-determining protein MinD (TIGR01968; HMM-score: 12.9)
  • TheSEED:  
    Amino Acids and DerivativesGlutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine, Glutamate, Aspartate and Asparagine Biosynthesis Glutamate racemase (EC 5.1.1.3) 
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis Glutamate racemase (EC 5.1.1.3) 
  • PFAM:
    Asp-glut_race (CL0399) Asp_Glu_race; Asp/Glu/Hydantoin racemase (PF01177; HMM-score: 117.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.104
    • Ymax_pos: 30
    • Cmax: 0.109
    • Cmax_pos: 21
    • Smax: 0.162
    • Smax_pos: 18
    • Smean: 0.089
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPRPGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTAIMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTITSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSALLTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]