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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01130
  • pan locus tag?: SAUPAN003431000
  • symbol: SAOUHSC_01130
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01130
  • symbol: SAOUHSC_01130
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1083817..1085307
  • length: 1491
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGATTTTATTCATTTTAACGGTTGTTGCAGTTATAGCAACATGGGTTATTCCTGCTGGT
    GCATATTCTAAACTTTCTTACGAACCTTCATCCCAAGAACTAAAGATAGTTAACCCTCAT
    AACCAAGTGAAAAAGGTTCCGGGTACGCAACAGGAACTAGACAAAATGGGGGTTAAAATT
    AAGATTGAACAATTTAAATCAGGTGCAATTAATAAGCCGGTATCAATTCCGAATACTTAT
    GAAAGATTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGT
    ACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTA
    GTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGG
    CATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGT
    GGTATTGAAGAAGAAGCTGTAGCATTTTATCCGATTTTAGTCCCTATATTTATAGCGTTA
    GGATACGATTCTATCGTTTCAGTTGGTGCCATATTCCTTGCCAGCTCTGTCGGTAGTACA
    TTTTCAACTATTAACCCGTTCTCGGTTGTAATTGCCTCTAATGCCGCTGGTACAACTTTT
    ACGGATGGCTTGTATTGGAGAATAGGTGCTTGTATTGTCGGTGCGATTTTTGTTATTAGT
    TATTTATATTGGTACTGTAAAAAAATTAAAAACGATCCTAAAGCGTCATATTCTTATGAA
    GACAAAGATGCTTTTGAACAGCAATGGTCTGTATTAAAAGATGACGATAGTGCCCATTTT
    ACTTTGCGTAAGAAGATAATCCTTACATTATTTGTACTACCATTTCCAATTATGGTATGG
    GGAGTTATGACGCAAGGTTGGTGGTTCCCAGTTATGGCTTCAGCATTTTTAATATTTACA
    ATTATAATAATGTTTATTGCTGGGACAGGTAAATCTGGATTGGGAGAAAAAGGAACTGTA
    GATGCATTTGTCAATGGTGCATCAAGTTTAGTAGGTGTATCTTTAATTATTGGTTTAGCT
    CGAGGTATTAATTTAGTGTTGAATGAAGGTATGATTTCAGATACAATCTTACACTTTTCA
    TCATCTTTAGTTCAACATATGAGTGGACCATTATTTATCATCGTATTACTATTTATTTTC
    TTCTGTTTAGGTTTTATCGTGCCATCATCTTCTGGCTTAGCGGTATTATCAATGCCTATC
    TTTGCACCACTAGCTGATACAGTAGGTATACCAAGATTCGTCATCGTTACGACATATCAA
    TTTGGTCAATATGCGATGTTATTCTTAGCGCCGACTGGACTTGTTATGGCCACACTACAA
    ATGTTAAACATGCGATATTCACATTGGTTCCGATTTGTATGGCCGGTAGTTGCTTTTGTA
    TTGATTTTCGGTGGCGGAGTACTAATTACGCAAGTACTAATTTATTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1491

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01130
  • symbol: SAOUHSC_01130
  • description: hypothetical protein
  • length: 496
  • theoretical pI: 7.56744
  • theoretical MW: 54069.7
  • GRAVY: 0.746976

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsTRAP transporter, DctM subunit (TIGR00786; HMM-score: 28.9)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine and Ornithine Degradation Arginine/ornithine antiporter ArcD 
    Arginine; urea cycle, polyaminesArginine Deiminase Pathway Arginine/ornithine antiporter ArcD 
    Arginine; urea cycle, polyaminesPolyamine Metabolism Arginine/ornithine antiporter ArcD 
  • PFAM:
    IT (CL0182) DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 583)
    DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 40.3)
    Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 21.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 22 aa
    • Ymax: 0.557
    • Ymax_pos: 22
    • Cmax: 0.615
    • Cmax_pos: 22
    • Smax: 0.625
    • Smax_pos: 19
    • Smean: 0.522
    • D: 0.543
  • predicted transmembrane helices (TMHMM): 13

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MILFILTVVAVIATWVIPAGAYSKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTYERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKTKGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASSVGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPKASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVMASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEGMISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTVGIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGGVLITQVLIYS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]