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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01216
  • pan locus tag?: SAUPAN003537000
  • symbol: sucC
  • pan gene symbol?: sucC
  • synonym:
  • product: succinyl-CoA synthetase subunit beta

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01216
  • symbol: sucC
  • product: succinyl-CoA synthetase subunit beta
  • replicon: chromosome
  • strand: +
  • coordinates: 1166886..1168052
  • length: 1167
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATATCCACGAGTATCAAGGTAAAGAAATATTTCGTTCAATGGGCGTTGCAGTTCCA
    GAAGGACGAGTAGCATTTACTGCTGAAGAAGCGGTGGAGAAAGCAAAAGAATTAAATTCT
    GATGTTTATGTTGTAAAAGCACAAATTCATGCTGGAGGTAGAGGTAAAGCAGGCGGAGTA
    AAAATTGCTAAATCTTTATCTGAGGTAGAAACATATGCAAAAGAATTATTAGGGAAAACT
    TTGGTGACACATCAAACTGGTCCAGAAGGTAAAGAAATTAAGCGTTTATATATCGAAGAA
    GGTTGTGCTATTCAAAAAGAATATTACGTTGGATTCGTTATTGATCGTGCGACTGACCAA
    GTAACATTGATGGCGTCTGAAGAAGGGGGCACTGAGATTGAAGAAGTTGCTGCGAAGACT
    CCTGAAAAGATCTTCAAAGAAACTATCGATCCAGTAATCGGACTTTCACCATTCCAAGCA
    AGACGAATTGCGTTTAATATTAATATTCCTAAAGAATCTGTTAACAAAGCTGCTAAATTC
    TTATTAGCACTTTATAATGTATTCATTGAAAAAGATTGTTCAATCGTAGAAATCAACCCA
    TTAGTTACAACAGCTGATGGTGATGTATTGGCATTAGATGCTAAAATTAATTTTGATGAT
    AATGCATTATTCAGACATAAAGATGTTGTAGAATTACGTGATTTAGAAGAAGAAGATCCG
    AAAGAGATTGAAGCGTCTAAACATGATTTATCATACATTGCATTAGATGGTGACATCGGA
    TGTATGGTTAATGGTGCAGGTTTAGCCATGGCAACAATGGATACGATTAATCATTTCGGT
    GGAAACCCAGCCAATTTCTTAGATGCAGGCGGAAGCGCTACTAGAGAAAAAGTAACTGAA
    GCATTTAAAATCATTTTAGGTGATGAAAATGTTAAAGGTATTTTTGTAAACATTTTCGGT
    GGCATTATGAAATGTGATGTTATCGCAGAAGGTATCGTTGAAGCTGTAAAAGAAGTAGAT
    TTAACTTTACCACTAGTTGTACGCCTAGAAGGTACAAATGTTGAGTTAGGTAAAAAAATC
    TTAAAAGACTCAGGATTAGCAATTGAACCAGCAGCAACAATGGCTGAAGGTGCACAAAAA
    ATTGTTAAACTAGTCAAAGAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1167

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01216
  • symbol: SucC
  • description: succinyl-CoA synthetase subunit beta
  • length: 388
  • theoretical pI: 4.63116
  • theoretical MW: 42056
  • GRAVY: -0.0510309

Function[edit source | edit]

  • reaction:
    EC 6.2.1.5?  ExPASy
    Succinate--CoA ligase (ADP-forming)ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
  • TIGRFAM:
    MetabolismEnergy metabolismTCA cyclesuccinate-CoA ligase, beta subunit (TIGR01016; EC 6.2.1.-; HMM-score: 545.1)
    acetyl coenzyme A synthetase (ADP forming), alpha domain (TIGR02717; EC 6.2.1.13; HMM-score: 21.8)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationputative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 17.4)
    Cellular processesCellular processesBiosynthesis of natural productscyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 15.3)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismDehydrogenase complexes Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) 
    Central carbohydrate metabolismTCA Cycle Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) 
    One-carbon MetabolismSerine-glyoxylate cycle Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) 
  • PFAM:
    ATP-grasp (CL0179) ATP-grasp_2; ATP-grasp domain (PF08442; HMM-score: 279.1)
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 84.9)
    ATP-grasp (CL0179) ATP-grasp_5; ATP-grasp domain (PF13549; HMM-score: 40.2)
    GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 17.4)
    Succ_CoA_synth (CL0506) Citrate_bind; ATP citrate lyase citrate-binding (PF16114; HMM-score: 12.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:
  • protein partners:
    SAOUHSC_00679hypothetical protein  [2] (data from MRSA252)
    SAOUHSC_00906hypothetical protein  [2] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 31
    • Cmax: 0.12
    • Cmax_pos: 31
    • Smax: 0.342
    • Smax_pos: 25
    • Smean: 0.145
    • D: 0.144
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYVVKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGCAIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQARRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINFDDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTINHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVEAVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. 2.0 2.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]