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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01330
  • pan locus tag?: SAUPAN003710000
  • symbol: SAOUHSC_01330
  • pan gene symbol?: guaC
  • synonym:
  • product: guanosine 5'-monophosphate oxidoreductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01330
  • symbol: SAOUHSC_01330
  • product: guanosine 5'-monophosphate oxidoreductase
  • replicon: chromosome
  • strand: +
  • coordinates: 1272663..1273640
  • length: 978
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGAAAATATTTGATTACGAAGATATTCAATTAATACCTAATAAATGCATAGTTGAAAGT
    AGGTCTGAATGTGATACAACTATCCAATTTGGTCCGAAAAAATTCAAGCTACCTGTAGTT
    CCTGCAAATATGCAAACAGTTATGAATGAGAAATTAGCGAAATGGTTTGCTGAAAATGAT
    TACTTTTATATCATGCATCGTTTTGATGAAGAAGCAAGAATACCTTTTATAAAACATATG
    CAAAATTCAGGCTTATTTGCATCTATTTCAGTTGGTGTAAAGAAAGCGGAATTTGATTTT
    ATTGAAAAGTTAGCTCAAGAAAAATTAATCCCCGAATATATTACAATAGATATTGCGCAT
    GGTCACTCAGATTCAGTGATAAACATGATTAAACATATAAAAACCCATATACCTGATAGT
    TTTGTTATTGCTGGTAATGTTGGTACGCCAGAAGGTGTTAGAGAATTAGAAAATGCTGGT
    GCTGATGCTACCAAAGTCGGTATAGGTCCTGGTAGAGTTTGTATTACAAAGATTAAAACA
    GGTTTTGGTACTGGTGGTTGGCAGTTAGCGGCATTAAACATATGTAGTAAAGCAGCTCGT
    AAACCTTTGATTGCCGATGGTGGTATAAGAACGCATGGCGACATTGCTAAATCAATTAGA
    TTTGGTGCATCAATGGTCATGATTGGTTCATTATTTGCGGCACACGAAGAATCACCTGGT
    GAAACTGTAGAACTTGATGGTAAACAGTATAAAGAATATTTTGGTAGTGCATCTGAATTT
    CAAAAAGGCGAACATAAAAATGTAGAAGGTAAAAAAATGTTTGTAGAACATAAGGGTTCA
    TTAATGGATACCTTAAAAGAAATGCAACAAGATTTACAAAGCTCAATTTCATATGCCGGT
    GGAAAAGACTTGAAATCATTACGTACTGTAGATTATGTTATTGTTAGAAACTCTATTTTC
    AACGGTGATAGAGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    978

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01330
  • symbol: SAOUHSC_01330
  • description: guanosine 5'-monophosphate oxidoreductase
  • length: 325
  • theoretical pI: 7.06414
  • theoretical MW: 36116.2
  • GRAVY: -0.279692

Function[edit source | edit]

  • reaction:
    EC 1.7.1.7?  ExPASy
    GMP reductaseInosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsguanosine monophosphate reductase (TIGR01306; EC 1.7.1.7; HMM-score: 701)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisinosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 186)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsguanosine monophosphate reductase (TIGR01305; EC 1.7.1.7; HMM-score: 175.2)
    Unknown functionGeneralIMP dehydrogenase family protein (TIGR01303; HMM-score: 139.4)
    Unknown functionGeneralIMP dehydrogenase family protein (TIGR01304; HMM-score: 58.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherisopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 31.5)
    glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 25.2)
    MetabolismAmino acid biosynthesisHistidine family1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 20.7)
    MetabolismAmino acid biosynthesisHistidine familyimidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 17.8)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 15.7)
    heme/flavin dehydrogenase, mycofactocin system (TIGR03966; EC 1.-.-.-; HMM-score: 14.3)
    putative enoyl-[acyl-carrier-protein] reductase II (TIGR03151; EC 1.3.1.-; HMM-score: 13.8)
    MetabolismAmino acid biosynthesisGlutamate family2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (TIGR01182; EC 4.1.2.14,4.1.3.16; HMM-score: 13.7)
    MetabolismEnergy metabolismEntner-Doudoroff2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (TIGR01182; EC 4.1.2.14,4.1.3.16; HMM-score: 13.7)
  • TheSEED:  
    Nucleosides and NucleotidesPurinesPurine conversions GMP reductase (EC 1.7.1.7) 
  • PFAM:
    TIM_barrel (CL0036) IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 227.9)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 26.8)
    FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 26.3)
    NMO; Nitronate monooxygenase (PF03060; HMM-score: 26.2)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 17.7)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 16.9)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 15.6)
    Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 13.4)
    Aldolase; KDPG and KHG aldolase (PF01081; HMM-score: 12.8)
    G3P_antiterm; Glycerol-3-phosphate responsive antiterminator (PF04309; HMM-score: 12.4)
    IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 11.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_00943(ppnK)inorganic polyphosphate/ATP-NAD kinase  [1] (data from MRSA252)
    SAOUHSC_01676hypothetical protein  [1] (data from MRSA252)
    SAOUHSC_01801isocitrate dehydrogenase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 57
    • Cmax: 0.107
    • Cmax_pos: 57
    • Smax: 0.183
    • Smax_pos: 51
    • Smean: 0.106
    • D: 0.112
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANMQTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIEKLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAGADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKSIRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVEHKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]