From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01452
  • pan locus tag?: SAUPAN003893000
  • symbol: SAOUHSC_01452
  • pan gene symbol?: ald1
  • synonym:
  • product: alanine dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01452
  • symbol: SAOUHSC_01452
  • product: alanine dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1409132..1410250
  • length: 1119
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTTAGTTGCAGTAGTCAAAGAATTAAAACAAGGTGAAGGACGTGTAGCTTGCACACCC
    GAAAATGTGCGTAAGTTAACGGATGCAGGACATAAAGTAATTGTTGAAAAAAATGCTGGC
    ATTGGTTCAGGATTTTCTAACGATATGTATGAAAAAGAAGGCGCTAAGATCGTAACTCAC
    GAACAAGCATGGGAAGCTGATCTTGTTATCAAAGTAAAAGAACCTCATGAAAGCGAATAT
    CAATATTTCAAAAAGAATCAAATTATCTGGGGATTTTTACATCTAGCATCTTCAAAAGAA
    ATAGTAGAAAAAATGCAAGAAGTTGGTGTAACTGCGATTAGTGGTGAAACCATTATAAAA
    AATGGAAAAGCAGAATTATTAGCGCCAATGAGTGCTATAGCAGGTCAACGCTCAGCAATT
    ATGGGAGCTTACTACTCTGAAGCACAACATGGTGGTCAAGGTACTTTAGTGACTGGTGTA
    CATGAAAATGTGGATATACCTGGTAGTACATATGTGATTTTCGGTGGTGGAGTAGCAGCA
    ACAAATGCAGCAAATGTTGCCTTGGGACTAAATGCTAAAGTAATCATTATCGAGTTAAAC
    GATGACCGCATTAAATATCTTGAAGATATGTATGCAGAAAAAGATGTCACAGTAGTCAAA
    TCAACACCAGAAAATTTAGCAGAACAAATTAAGAAAGCAGATGTATTTATTTCTACAATT
    TTAATTTCAGGTGCGAAACCGCCAAAATTGGTTACTCGTGAGATGGTTAAATCAATGAAA
    AAAGGTTCAGTATTAATCGATATAGCTATTGACCAAGGTGGAACTATTGAAACAATTAGA
    CCAACTACAATTTCTGATCCAGTGTATGAAGAAGAAGGTGTGATTCATTATGGTGTACCA
    AATCAACCAGGAGCAGTCCCAAGAACTTCAACAATGGCATTAGCACAAGGAAATATTGAT
    TATATATTAGAAATTTGTGACAAAGGCTTAGAACAAGCAATTAAAGATAATGAAGCCTTA
    AGTACTGGTGTAAACATTTACCAAGGACAAGTGACAAATCAAGGATTAGCTTCATCACAT
    GACCTAGATTATAAAGAAATATTAAATGTTATCGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1119

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01452
  • symbol: SAOUHSC_01452
  • description: alanine dehydrogenase
  • length: 372
  • theoretical pI: 4.89305
  • theoretical MW: 40224.6
  • GRAVY: -0.138172

⊟Function[edit | edit source]

  • reaction:
    EC 1.4.1.1?  ExPASy
    Alanine dehydrogenase L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 393.6)
    and 6 more
    Metabolism Energy metabolism Electron transport NAD(P)(+) transhydrogenase (AB-specific), alpha subunit (TIGR00561; EC 1.6.1.2; HMM-score: 137.2)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 23)
    Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 19)
    methylmalonyl-CoA mutase C-terminal domain (TIGR00640; HMM-score: 16.4)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 14.3)
    Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 14)
  • TheSEED  :
    • Alanine dehydrogenase (EC 1.4.1.1)
    Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  Alanine dehydrogenase (EC 1.4.1.1)
  • PFAM:
    NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 251)
    and 11 more
    Form_Glyc_dh (CL0325) AlaDh_PNT_N; Alanine dehydrogenase/PNT, N-terminal domain (PF05222; HMM-score: 142.3)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 26.4)
    THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 25.1)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 23.4)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 20.7)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 17.2)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 15.2)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 14.9)
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 14)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 13.5)
    Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011205
    • TAT(Tat/SPI): 0.000954
    • LIPO(Sec/SPII): 0.005196
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSGFSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIVEKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGVHENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTVVKSTPENLAEQIKKADVFISTILISGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTIETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAIKDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Rex* (repression) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_01452&oldid=92806"