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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01462
  • pan locus tag?: SAUPAN003904000
  • symbol: SAOUHSC_01462
  • pan gene symbol?: gpsB
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01462
  • symbol: SAOUHSC_01462
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1419125..1419469
  • length: 345
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    ATGTCAGATGTTTCATTGAAATTATCAGCAAAAGATATTTATGAAAAAGATTTTGAAAAA
    ACGATGGCTCGTGGCTATAGAAGAGAAGAAGTAGATGCATTTTTAGATGACATTATTGCT
    GATTATCAAAAAATGGCCGATATGAATAATGAAGTTGTAAAATTATCAGAAGAGAATCAT
    AAACTTAAAAAAGAATTAGAAGAATTAAGACTACGTGTAGCAACATCAAGACCTCAGGAC
    AATAAAAGTTTTTCTTCGAATAATACAACAACAAATACATCTTCAAATAATGTAGATATT
    TTAAAACGTATTTCAAACTTAGAAAAAGCTGTATTTGGTAAATAA
    60
    120
    180
    240
    300
    345

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01462
  • symbol: SAOUHSC_01462
  • description: hypothetical protein
  • length: 114
  • theoretical pI: 5.97468
  • theoretical MW: 13150.7
  • GRAVY: -0.867544

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    DivIVA domain (TIGR03544; HMM-score: 52.8)
    Cellular processesCellular processesSporulation and germinationtranscription factor, RsfA family (TIGR02894; HMM-score: 16.8)
    Signal transductionRegulatory functionsDNA interactionstranscription factor, RsfA family (TIGR02894; HMM-score: 16.8)
    DivIVA domain repeat protein (TIGR03543; HMM-score: 16.1)
    SH3 domain protein (TIGR04211; HMM-score: 13.5)
  • TheSEED:  
    Cell Division and Cell CycleCell Division and Cell Cycle - no subcategoryControl of cell elongation - division cycle in Bacilli Cell division protein GpsB, coordinates the switch between cylindrical and septal cell wall synthesis by re-localization of PBP1 
  • PFAM:
    no clan definedDivIVA; DivIVA protein (PF05103; HMM-score: 45.9)
    YabB; Initiation control protein YabA (PF06156; HMM-score: 24.9)
    HMMR_C; Hyaluronan mediated motility receptor C-terminal (PF15908; HMM-score: 19.7)
    Ax_dynein_light; Axonemal dynein light chain (PF10211; HMM-score: 18.9)
    bZIP (CL0018) bZIP_1; bZIP transcription factor (PF00170; HMM-score: 16.7)
    no clan definedHOOK; HOOK protein (PF05622; HMM-score: 16.3)
    CtIP_N; Tumour-suppressor protein CtIP N-terminal domain (PF10482; HMM-score: 15.8)
    FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 15.7)
    no clan definedABC_tran_CTD; ABC transporter C-terminal domain (PF16326; HMM-score: 15)
    DASH_Spc19; Spc19 (PF08287; HMM-score: 14.9)
    HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.7)
    SlyX; SlyX (PF04102; HMM-score: 14.5)
    CENP-H; Centromere protein H (CENP-H) (PF05837; HMM-score: 14.2)
    Ribo_L29 (CL0346) Ribosomal_L29; Ribosomal L29 protein (PF00831; HMM-score: 13.8)
    no clan definedTolA_bind_tri; TolA binding protein trimerisation (PF16331; HMM-score: 13.7)
    bZIP (CL0018) bZIP_2; Basic region leucine zipper (PF07716; HMM-score: 13.3)
    no clan definedCnn_1N; Centrosomin N-terminal motif 1 (PF07989; HMM-score: 12.5)
    IncA; IncA protein (PF04156; HMM-score: 12.3)
    Herpes_BLRF2; Herpesvirus BLRF2 protein (PF05812; HMM-score: 12)
    CC2-LZ; Leucine zipper of domain CC2 of NEMO, NF-kappa-B essential modulator (PF16516; HMM-score: 10.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.103
    • Ymax_pos: 65
    • Cmax: 0.105
    • Cmax_pos: 56
    • Smax: 0.117
    • Smax_pos: 53
    • Smean: 0.075
    • D: 0.092
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQKMADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILKRISNLEKAVFGK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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