From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01470
  • pan locus tag?: SAUPAN003913000
  • symbol: SAOUHSC_01470
  • pan gene symbol?: dnaD
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01470
  • symbol: SAOUHSC_01470
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1425296..1425982
  • length: 687
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGATAAATATCAATTAAAAGCAAGACCTGTAGTGATACGAAGAGAATTATTAGACCAT
    TACAGTGACTTAGGTTTAGATGAGCAAGATTTAGTCATTTTGCTTAAATTGATTTATGCT
    TCCGAAACATCAAATAAACAGCCTTCAATTGAATTGCTTCAGAAGGGCTCAACTATGCAA
    CCACGTGATATTACAATGGTTATTCAAAATTTGATACAACGTGAATTGCTAGAATTACAA
    GTTCAAAAAGATGAAGAAGGTAGATTTACCGAATATATGAATCTAGATCCTTTTTTCGAA
    AAATTAAGTCATATATTGAAGCAACAAAGTATGGAAACGAAAGAGCTAAATAGTAAAGAA
    AAATTTAAACAATTATTTAGAGTTTTAGAGGATACATTTGCAAGGCCGTTATCTCCATAT
    GAAATAGAAACGTTAAATCAATGGATTGATGTTGATAAACACGATACTGCAATTATACAA
    GCGGCATTAGACGAGGCGAATAGTTTGAATAAACTTAGCTTTAAATATATAGATCGTATT
    TTACTTAATTGGAAGAAAAACAATGTCAAAACAATTGACGATTCTAGAAAAATAAGAGAA
    AAATTCAATAAACCGAAAATGACGCACACAGTTAAAACGGTACCTAAATTTGATTGGTTA
    AACGGGGAGAACCTTGATGGTAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    687

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01470
  • symbol: SAOUHSC_01470
  • description: hypothetical protein
  • length: 228
  • theoretical pI: 6.98192
  • theoretical MW: 26954.8
  • GRAVY: -0.657456

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDnaD domain protein (TIGR01446; HMM-score: 78.3)
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsDnaD domain protein (TIGR01446; HMM-score: 78.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDnaD family protein (TIGR04548; HMM-score: 39)
    Signal transductionRegulatory functionsDNA interactionsstaphylococcal accessory regulator family (TIGR01889; HMM-score: 10.6)
  • TheSEED:  
    Control of cell elongation - division cycle in Bacilli Chromosome replication initiation protein DnaD 
  • PFAM:
    no clan definedDnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 76.7)
    Membr_traf_MHD; Munc13 (mammalian uncoordinated) homology domain (PF10540; HMM-score: 15.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.109
    • Ymax_pos: 11
    • Cmax: 0.117
    • Cmax_pos: 47
    • Smax: 0.218
    • Smax_pos: 3
    • Smean: 0.118
    • D: 0.113
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETSNKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKLSHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQAALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFDWLNGENLDGK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]