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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01563
  • pan locus tag?: SAUPAN001439000
  • symbol: SAOUHSC_01563
  • pan gene symbol?:
  • synonym:
  • product: phage encoded DNA polymerase I

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01563
  • symbol: SAOUHSC_01563
  • product: phage encoded DNA polymerase I
  • replicon: chromosome
  • strand: -
  • coordinates: 1497709..1499661
  • length: 1953
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATATAGATATTGAAACATACAGCAGTAACGATATTTCGAAATGTGGTGCCTATAAA
    TACACAGAAGCTGAAGATTTCGAAATTTTAATTATAGCTTATTCGATAGATGGTGGAGCG
    ATTAGTGCGATTGACATGACTAAAGTAGATAATGAGCCTTTCCACGCTGATTATGAGACG
    TTTAAAATTGCTCTATTTGACCCTGCTGTAAAAAAGTATGCATTCAATGCTAATTTCGAA
    AGAACTTGTCTTGCTAAACATTTTAATAAACAGATGCCACCTGAAGAATGGATTTGCACA
    ATGGTTAATTCAATGCGTATTGGCTTACCTGCTTCGCTTGATAAAGTTGGAGAAGTTTTA
    AGACTACAAAGCCAAAAAGATAAAGCAGGTAAAAATTTAATTCGTTATTTCTCTATACCT
    TGTAAACCAACAAAAGTTAATGGAGGAAGAACAAGAAACCTACCTGAACATGATCTTGAA
    AAATGGCAACAATTTATAGATTACTGTATTCGAGATGTAGAAGTAGAAATGGCGATTGCT
    AATAAAATTAAAGACTTTCCAGTAACTGCAATTGAACAAACATATTGGGTTTTTGACCAA
    CATATAAACGACAGAGGTATTAAGCTTTCTAAATCATTGATGTTAGGAGCTAATGTGCTC
    GATAAGCAGAGTAAAGAAGAATTGCTTAAACAAGCTAAACATATAACAGGTTTAGAAAAT
    CCTAATAGTCCTACACAGTTATTGGCTTGGTTAAAGGATGAACAAGGATTAGATATACCT
    AATTTACAAAAGAAAACGGTTCAGGAGTACTTAAAAGAAGCAACAGGAAAAGCTAAAAAA
    ATGCTAGAAATTAGATTGCAAATGTCTAAAACCAGTGTGAAAAAATACAACAAAATGCAT
    GACATGATGTGCAGTGATGAACGGGTAAGAGGTCTGTTTCAATTTTACGGTGCCGGTACT
    GGAAGATGGGCAGGTAGAGGTGTACAACTTCAGAATTTAACAAAGCATTATATTTCAGAT
    ACTGAATTAGAAATAGCAAGAGATCTTATTAAAGAACAACGTTTTGATGATTTAGATTTA
    TTACTCAATGTTCATCCTCAAGACTTATTAAGTCAATTAGTTAGGACGACATTTACTGCT
    GAAGAAGGTAATGAACTAGCAGTAAGTGATTTTTCTGCAATAGAGGCAAGAGTCATAGCA
    TGGTATGCAAAAGAACAATGGCGTTTAGATGTATTCAACACACACGGAAAGATATATGAA
    GCATCGGCTTCTCAAATGTTTAATGTACCGGTAGAAAGCATAACTAAAGGCGACCCTCTC
    AGACAAAAAGGAAAAGTGTCCGAATTAGCTTTAGGCTATCAAGGTGGCGCTGGAGCTTTA
    AAAGCAATGGGTGCATTGGAAATGGGCATTGAAGAAAACGAGTTACAAGGTTTAGTTGAT
    AGTTGGCGTAACGCAAATCCTAACATAGTTAATTTTTGGAAGGCTTGCCAAGAGGCTGCA
    ATTAATACTGTAAAATCCCGAAAGACGCATCATACACATGGACTTAGATTTTATATGAAA
    AAAGGTTTTCTAATGATTGAACTGCCTAGTGGAAGAGCTTTAGCTTATCCAAAAGCTTTA
    GTTGGTGAAAATAGTTGGGGTAGTCAAGTTGTTGAATTTATGGGGTTAGATCTTAACCGT
    AAATGGTCAAAGTTAAAAACGTATGGTGGGAAGTTAGTCGAGAATATTGTTCAAGCAACT
    GCAAGGGATTTACTTGCGATTTCTATAGCAAGGCTTGAAGCATTAGGTTTTAAAATAGTT
    GGCCATGTCCATGATGAAGTAATTGTAGAAATACCTAGAGGTTCAAATGGACTTAAGGAA
    ATCGAAACTATCATGAATAAGCCTGTTGATTGGGCAAAAGGATTGAATTTGAATAGTGAC
    GGGTTTACTTCTCCGTTTTATATGAAGGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1953

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01563
  • symbol: SAOUHSC_01563
  • description: phage encoded DNA polymerase I
  • length: 650
  • theoretical pI: 7.39229
  • theoretical MW: 73568.9
  • GRAVY: -0.38

Function[edit source | edit]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymeraseDeoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA polymerase I (TIGR00593; EC 2.7.7.7; HMM-score: 54.4)
  • TheSEED:  
    DNA MetabolismDNA repairDNA Repair Base Excision DNA polymerase I (EC 2.7.7.7) 
    DNA replicationDNA-replication DNA polymerase I (EC 2.7.7.7) 
  • PFAM:
    no clan definedDNA_pol_A; DNA polymerase family A (PF00476; HMM-score: 88.2)
    RNase_H (CL0219) DNA_pol_A_exo1; 3'-5' exonuclease (PF01612; HMM-score: 14.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 57
    • Cmax: 0.163
    • Cmax_pos: 57
    • Smax: 0.103
    • Smax_pos: 1
    • Smean: 0.069
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAIDMTKVDNEPFHADYETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMVNSMRIGLPASLDKVGEVLRLQSQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLEKWQQFIDYCIRDVEVEMAIANKIKDFPVTAIEQTYWVFDQHINDRGIKLSKSLMLGANVLDKQSKEELLKQAKHITGLENPNSPTQLLAWLKDEQGLDIPNLQKKTVQEYLKEATGKAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLTKHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFSAIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELALGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRKTHHTHGLRFYMKKGFLMIELPSGRALAYPKALVGENSWGSQVVEFMGLDLNRKWSKLKTYGGKLVENIVQATARDLLAISIARLEALGFKIVGHVHDEVIVEIPRGSNGLKEIETIMNKPVDWAKGLNLNSDGFTSPFYMKD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]